Identification, functional characterization and expression patterns of a water-specific aquaporin in the brown dog tick, Rhipicephalus sanguineus

Andrew Ball, Ewan M Campbell, Jimmy Jacob, Stefan Hoppler, Alan S Bowman (Corresponding Author)

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Much is known about the physiology of tick salivation, but nothing is known about the movement of water through the cell membranes of salivary glands, a phenomenon usually associated with water channels or aquaporins (AQPs). An AQP, RsAQP1, was identified in a salivary gland cDNA library of Rhipicephalus sanguineus. In the first functional characterization of an acarine AQP, Xenopus oocytes expressing RsAQP1 became water permeable, whereas RsAQP1 did not transport glycerol or urea. RsAQP1 was inhibited by Hg2+ but not by triethylammonium. Treatment with a protein kinase A activator (cAMP) had no effect on RsAQP1 transport, whereas treatment with a protein kinase C activator (phorbol 12,13-dibutyrate) reduced water flux by 60%. RsAQP1 transcript was present in unfed larvae, nymphs and adult R. sanguineus, but absent in embryos. Partially fed female R. sanguineus expressed RsAQP1 in gut, Malpighian tubules and was particularly abundant in salivary gland tissue, but absent in ovary and synganglion tissues. Because of the importance of water management in tick biology for both the off-host and on-host phases of the life cycle, our findings on tick AQP1 represent a major advancement in our understanding of tick osmoregulation that could potentially be exploited in tick control. (c) 2008 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalInsect Biochemistry and Molecular Biology
Volume39
Issue number2
DOIs
Publication statusPublished - Feb 2009

Keywords

  • tick
  • rhipicephalus sanguineus
  • aquaporin
  • osmoregulation
  • fluid, salivary gland
  • malpighian tubule
  • phosphorylation
  • lone star tick
  • amblyomma-americanum L
  • protein-kinase-C
  • salivary-glands
  • xenopus-oocytes
  • ixodid ticks
  • channel
  • appendiculatis
  • permeability

Cite this

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title = "Identification, functional characterization and expression patterns of a water-specific aquaporin in the brown dog tick, Rhipicephalus sanguineus",
abstract = "Much is known about the physiology of tick salivation, but nothing is known about the movement of water through the cell membranes of salivary glands, a phenomenon usually associated with water channels or aquaporins (AQPs). An AQP, RsAQP1, was identified in a salivary gland cDNA library of Rhipicephalus sanguineus. In the first functional characterization of an acarine AQP, Xenopus oocytes expressing RsAQP1 became water permeable, whereas RsAQP1 did not transport glycerol or urea. RsAQP1 was inhibited by Hg2+ but not by triethylammonium. Treatment with a protein kinase A activator (cAMP) had no effect on RsAQP1 transport, whereas treatment with a protein kinase C activator (phorbol 12,13-dibutyrate) reduced water flux by 60{\%}. RsAQP1 transcript was present in unfed larvae, nymphs and adult R. sanguineus, but absent in embryos. Partially fed female R. sanguineus expressed RsAQP1 in gut, Malpighian tubules and was particularly abundant in salivary gland tissue, but absent in ovary and synganglion tissues. Because of the importance of water management in tick biology for both the off-host and on-host phases of the life cycle, our findings on tick AQP1 represent a major advancement in our understanding of tick osmoregulation that could potentially be exploited in tick control. (c) 2008 Elsevier Ltd. All rights reserved.",
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author = "Andrew Ball and Campbell, {Ewan M} and Jimmy Jacob and Stefan Hoppler and Bowman, {Alan S}",
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T1 - Identification, functional characterization and expression patterns of a water-specific aquaporin in the brown dog tick, Rhipicephalus sanguineus

AU - Ball, Andrew

AU - Campbell, Ewan M

AU - Jacob, Jimmy

AU - Hoppler, Stefan

AU - Bowman, Alan S

PY - 2009/2

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N2 - Much is known about the physiology of tick salivation, but nothing is known about the movement of water through the cell membranes of salivary glands, a phenomenon usually associated with water channels or aquaporins (AQPs). An AQP, RsAQP1, was identified in a salivary gland cDNA library of Rhipicephalus sanguineus. In the first functional characterization of an acarine AQP, Xenopus oocytes expressing RsAQP1 became water permeable, whereas RsAQP1 did not transport glycerol or urea. RsAQP1 was inhibited by Hg2+ but not by triethylammonium. Treatment with a protein kinase A activator (cAMP) had no effect on RsAQP1 transport, whereas treatment with a protein kinase C activator (phorbol 12,13-dibutyrate) reduced water flux by 60%. RsAQP1 transcript was present in unfed larvae, nymphs and adult R. sanguineus, but absent in embryos. Partially fed female R. sanguineus expressed RsAQP1 in gut, Malpighian tubules and was particularly abundant in salivary gland tissue, but absent in ovary and synganglion tissues. Because of the importance of water management in tick biology for both the off-host and on-host phases of the life cycle, our findings on tick AQP1 represent a major advancement in our understanding of tick osmoregulation that could potentially be exploited in tick control. (c) 2008 Elsevier Ltd. All rights reserved.

AB - Much is known about the physiology of tick salivation, but nothing is known about the movement of water through the cell membranes of salivary glands, a phenomenon usually associated with water channels or aquaporins (AQPs). An AQP, RsAQP1, was identified in a salivary gland cDNA library of Rhipicephalus sanguineus. In the first functional characterization of an acarine AQP, Xenopus oocytes expressing RsAQP1 became water permeable, whereas RsAQP1 did not transport glycerol or urea. RsAQP1 was inhibited by Hg2+ but not by triethylammonium. Treatment with a protein kinase A activator (cAMP) had no effect on RsAQP1 transport, whereas treatment with a protein kinase C activator (phorbol 12,13-dibutyrate) reduced water flux by 60%. RsAQP1 transcript was present in unfed larvae, nymphs and adult R. sanguineus, but absent in embryos. Partially fed female R. sanguineus expressed RsAQP1 in gut, Malpighian tubules and was particularly abundant in salivary gland tissue, but absent in ovary and synganglion tissues. Because of the importance of water management in tick biology for both the off-host and on-host phases of the life cycle, our findings on tick AQP1 represent a major advancement in our understanding of tick osmoregulation that could potentially be exploited in tick control. (c) 2008 Elsevier Ltd. All rights reserved.

KW - tick

KW - rhipicephalus sanguineus

KW - aquaporin

KW - osmoregulation

KW - fluid, salivary gland

KW - malpighian tubule

KW - phosphorylation

KW - lone star tick

KW - amblyomma-americanum L

KW - protein-kinase-C

KW - salivary-glands

KW - xenopus-oocytes

KW - ixodid ticks

KW - channel

KW - appendiculatis

KW - permeability

U2 - 10.1016/j.ibmb.2008.10.006

DO - 10.1016/j.ibmb.2008.10.006

M3 - Article

VL - 39

SP - 105

EP - 112

JO - Insect Biochemistry and Molecular Biology

JF - Insect Biochemistry and Molecular Biology

SN - 0965-1748

IS - 2

ER -