Identification of a bisphosphonate that inhibits isopentenyl diphosphate isomerase and farnesyl diphosphate synthase

Keith Thompson, James Edward Dunford, F. H. Ebetino, Michael John Rogers

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

We and others have recently shown that the major molecular target of nitrogen-containing bisphosphonate drugs is farnesyl diphosphate synthase, an enzyme in the mevalonate pathway. In an in vitro screen, we discovered a bisphosphonate, NE21650, that potently inhibited farnesyl diphosphate synthase but, unlike other N-BPs investigated, was also a weak inhibitor of isopentenyl diphosphate isomerase. NE21650 was a more potent inhibitor of protein prenylation in osteoclasts and macrophages, and a more potent inhibitor of bone resorption in vitro, than alendronate, despite very similar IC50 values for inhibition of farnesyl diphosphate synthase. Our observations show that minor changes to the structure of bisphosphonates allow inhibition of more than one enzyme in the mevalonate pathway and suggest that loss of protein prenylation due to inhibition of more than one enzyme in the mevalonate pathway may lead to an increase in antiresorptive potency compared to bisphosphonates that only inhibit farnesyl diphosphate synthase. (C) 2002 Elsevier Science.

Original languageEnglish
Pages (from-to)869-873
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume290
Issue number2
DOIs
Publication statusPublished - 18 Jan 2002

Keywords

  • bisphosphonate
  • farnesyl diphosphate synthase
  • isopentenyl diphosphate isomerase
  • mevalonate
  • prenylation
  • osteoclast
  • nitrogen-containing bisphosphonates
  • mevalonate pathway
  • osteoclast formation
  • bone-resorption
  • in-vitro
  • geranylation
  • alendronate
  • activation
  • mechanism
  • kinase

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