Identification of a developmentally regulated Trichinella spiralis protein that inhibits MyoD-specific protein

J G Lindh, Bernadette Connolly, D L McGhie, D F Smith

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Development of the infective L1 larva of Trichinella spiralis occurs as an intracellular parasite of skeletal muscle and leads to the dedifferentiation of the host cell. A novel Trichinella gene, tsJ5, has been identified from a cDNA library screen for sequences encoding Trichinella proteins related to the myogenic bHLH factors. The tsJ5 gene is developmentally regulated, showing preferential expression in the infective muscle stage larva. The product of the tsJ5 gene is not a bHLH protein but represents a novel protein with properties in common with some myogenic repressors. A recombinant TsJ5 protein affects the formation of MyoD:DNA complexes in vitro.
Original languageEnglish
Pages (from-to)163-175
Number of pages13
JournalMolecular and Biochemical Parasitology
Volume92
Issue number1
DOIs
Publication statusPublished - 1 Apr 1998

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Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA, Complementary
  • Gene Expression Regulation
  • Gene Library
  • Genes, Helminth
  • Helminth Proteins
  • Larva
  • Molecular Sequence Data
  • Muscle Proteins
  • MyoD Protein
  • Protein Binding
  • Repressor Proteins
  • Sequence Homology, Amino Acid
  • Trichinella spiralis
  • Trichinella
  • Infective stage larva
  • TsJ5
  • Inhibitor of MyoD
  • Developmental regulation

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