Abstract
A novel IL-1 family member (nIL-IF) has been discovered in fish, adding a further member to this cytokine family. The unique gene organization of nIL-1F, together with its location in the genome and low homology to known family members, suggests that this molecule is not homologous to known IL-1F. Nevertheless, it contains a predicted C-terminal beta-trefoil structure, an IL-1F signature region within the final exon, a potential IL-1 converting enzyme cut site, and its expression level is clearly increased following infection, or stimulation of macrophages with LPS or IL-1 beta. A thrombin cut site is also present and may have functional relevance. The C-terminal recombinant protein antagonized the effects of rainbow trout rIL-1 beta on inflammatory gene expression in a trout macrophage cell line, suggesting it is an IL-1 beta antagonist. Modeling studies confirmed that nIL-IF has the potential to bind to the trout IL-1RI receptor protein, and may be a novel IL-1 receptor antagonist. The Journal of Immunology, 2009, 183: 962-974.
Original language | English |
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Pages (from-to) | 962-974 |
Number of pages | 13 |
Journal | The Journal of Immunology |
Volume | 183 |
Issue number | 2 |
Early online date | 24 Jun 2009 |
DOIs | |
Publication status | Published - 15 Jul 2009 |
Keywords
- trout oncorhynchus-mykiss
- receptor antagonist gene
- rainbow-trout
- functional-characterization
- molecular-cloning
- crystal-structure
- computer-program
- DNA-sequences
- genomic DNA
- interleukin-1