Identification of a novel IL-1 cytokine family member in teleost fish

Tiehui Wang, Steve Bird, Antonis Koussounadis, Jason W Holland, Allison Carrington, Jun Zou, Christopher J Secombes

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

A novel IL-1 family member (nIL-IF) has been discovered in fish, adding a further member to this cytokine family. The unique gene organization of nIL-1F, together with its location in the genome and low homology to known family members, suggests that this molecule is not homologous to known IL-1F. Nevertheless, it contains a predicted C-terminal beta-trefoil structure, an IL-1F signature region within the final exon, a potential IL-1 converting enzyme cut site, and its expression level is clearly increased following infection, or stimulation of macrophages with LPS or IL-1 beta. A thrombin cut site is also present and may have functional relevance. The C-terminal recombinant protein antagonized the effects of rainbow trout rIL-1 beta on inflammatory gene expression in a trout macrophage cell line, suggesting it is an IL-1 beta antagonist. Modeling studies confirmed that nIL-IF has the potential to bind to the trout IL-1RI receptor protein, and may be a novel IL-1 receptor antagonist. The Journal of Immunology, 2009, 183: 962-974.

Original languageEnglish
Pages (from-to)962-974
Number of pages13
JournalThe Journal of Immunology
Volume183
Issue number2
Early online date24 Jun 2009
DOIs
Publication statusPublished - 15 Jul 2009

Keywords

  • trout oncorhynchus-mykiss
  • receptor antagonist gene
  • rainbow-trout
  • functional-characterization
  • molecular-cloning
  • crystal-structure
  • computer-program
  • DNA-sequences
  • genomic DNA
  • interleukin-1

Cite this

Identification of a novel IL-1 cytokine family member in teleost fish. / Wang, Tiehui; Bird, Steve; Koussounadis, Antonis; Holland, Jason W; Carrington, Allison; Zou, Jun; Secombes, Christopher J.

In: The Journal of Immunology, Vol. 183, No. 2, 15.07.2009, p. 962-974.

Research output: Contribution to journalArticle

Wang, Tiehui ; Bird, Steve ; Koussounadis, Antonis ; Holland, Jason W ; Carrington, Allison ; Zou, Jun ; Secombes, Christopher J. / Identification of a novel IL-1 cytokine family member in teleost fish. In: The Journal of Immunology. 2009 ; Vol. 183, No. 2. pp. 962-974.
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abstract = "A novel IL-1 family member (nIL-IF) has been discovered in fish, adding a further member to this cytokine family. The unique gene organization of nIL-1F, together with its location in the genome and low homology to known family members, suggests that this molecule is not homologous to known IL-1F. Nevertheless, it contains a predicted C-terminal beta-trefoil structure, an IL-1F signature region within the final exon, a potential IL-1 converting enzyme cut site, and its expression level is clearly increased following infection, or stimulation of macrophages with LPS or IL-1 beta. A thrombin cut site is also present and may have functional relevance. The C-terminal recombinant protein antagonized the effects of rainbow trout rIL-1 beta on inflammatory gene expression in a trout macrophage cell line, suggesting it is an IL-1 beta antagonist. Modeling studies confirmed that nIL-IF has the potential to bind to the trout IL-1RI receptor protein, and may be a novel IL-1 receptor antagonist. The Journal of Immunology, 2009, 183: 962-974.",
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