Identification of a second group of type I IFNs in fish sheds light on IFN evolution in vertebrates

Jun Zou, Carolina Tafalla, Jonathan Truckle, Chris J. Secombes

Research output: Contribution to journalArticle

222 Citations (Scopus)

Abstract

In this report, three type I IFN genes were identified in rainbow trout (rt) Oncorhynchus mykiss and are classified into two groups based on their primary protein sequences: group I containing two cysteine residues; and group II containing four cysteines residues. The group I rtIFNs were induced in fibroblasts (RTG-2 cells), macrophages (RTS-11 cells), and head kidney leukocytes when stimulated with polyinosinic:polycytidylic acid, whereas group II IFN was up-regulated in head kidney leukocytes but not in RTG-2 and RTS-11 cells. Recombinant group I rtIFNs were potent at inducing Mx expression and eliciting antiviral responses, whereas recombinant group II rtIFN was poor in these activities. That two subgroups of type I IFN exist in trout prompted a survey of the genomes of several fish species, including zebrafish, medaka, threespine stickleback and fugu, the amphibian Xenopus tropicalis, the monotreme platypus and the marsupial opossum, to gain further insight into possible IFN evolution. Analysis of the sequences confirmed that the new IFN subgroup found in trout (group II IFN) exists in other fish species but was not universally present in fish. The IFN genes in amphibians were shown for the first time to contain introns and to conserve the four cysteine structure found in all type I IFNs except IFN-/beta epsilon and fish group I IFN. The data overall support the concept that different vertebrate groups have independently expanded their IFN types, with deletion of different pairs of cysteines apparent in fish group I IFN and IFN-/beta epsilon of mammals.

Original languageEnglish
Pages (from-to)3859-3871
Number of pages13
JournalThe Journal of Immunology
Volume179
Issue number6
Publication statusPublished - 15 Sep 2007

Keywords

  • comparative genomic analysis
  • interferon gene family
  • teleost fish
  • cell-line
  • receptor
  • sequence
  • system
  • resolution
  • zebrafish
  • mammals

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