Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli

S  Miller; L S  Ness; C M  Wood; B C  Fox; I R  Booth

Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli

S Miller, L S Ness, C M Wood, B C Fox, I R Booth

Medical Sciences

Research output: Contribution to journal › Article

34 Citations (Scopus)

Abstract

A new subunit, YabF, for the KefC K+ efflux system in Escherichia call has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations.

Original language	English
Pages (from-to)	6536-6540
Number of pages	5
Journal	Journal of Bacteriology
Volume	182
Publication status	Published - 2000

Keywords

LINKAGE MAP
EDITION 10
CHANNELS
ACTIVATION
CLONING
GENE
K-12
TRANSPORT
SEQUENCE

Cite this

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title = "Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli",

abstract = "A new subunit, YabF, for the KefC K+ efflux system in Escherichia call has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations.",

keywords = "LINKAGE MAP, EDITION 10, CHANNELS, ACTIVATION, CLONING, GENE, K-12, TRANSPORT, SEQUENCE",

author = "S Miller and Ness, {L S} and Wood, {C M} and Fox, {B C} and Booth, {I R}",

year = "2000",

language = "English",

volume = "182",

pages = "6536--6540",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

}

TY - JOUR

T1 - Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli

AU - Miller, S

AU - Ness, L S

AU - Wood, C M

AU - Fox, B C

AU - Booth, I R

PY - 2000

Y1 - 2000

N2 - A new subunit, YabF, for the KefC K+ efflux system in Escherichia call has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations.

AB - A new subunit, YabF, for the KefC K+ efflux system in Escherichia call has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations.

KW - LINKAGE MAP

KW - EDITION 10

KW - CHANNELS

KW - ACTIVATION

KW - CLONING

KW - GENE

KW - K-12

KW - TRANSPORT

KW - SEQUENCE

M3 - Article

SN - 0021-9193

VL - 182

SP - 6536

EP - 6540

JO - Journal of Bacteriology

JF - Journal of Bacteriology

ER -

Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli

Abstract

Keywords

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