Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli

S Miller, L S Ness, C M Wood, B C Fox, I R Booth

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A new subunit, YabF, for the KefC K+ efflux system in Escherichia call has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations.

Original languageEnglish
Pages (from-to)6536-6540
Number of pages5
JournalJournal of Bacteriology
Volume182
Publication statusPublished - 2000

Keywords

  • LINKAGE MAP
  • EDITION 10
  • CHANNELS
  • ACTIVATION
  • CLONING
  • GENE
  • K-12
  • TRANSPORT
  • SEQUENCE

Cite this

Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli. / Miller, S ; Ness, L S ; Wood, C M ; Fox, B C ; Booth, I R .

In: Journal of Bacteriology, Vol. 182, 2000, p. 6536-6540.

Research output: Contribution to journalArticle

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abstract = "A new subunit, YabF, for the KefC K+ efflux system in Escherichia call has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations.",
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T1 - Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli

AU - Miller, S

AU - Ness, L S

AU - Wood, C M

AU - Fox, B C

AU - Booth, I R

PY - 2000

Y1 - 2000

N2 - A new subunit, YabF, for the KefC K+ efflux system in Escherichia call has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations.

AB - A new subunit, YabF, for the KefC K+ efflux system in Escherichia call has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations.

KW - LINKAGE MAP

KW - EDITION 10

KW - CHANNELS

KW - ACTIVATION

KW - CLONING

KW - GENE

KW - K-12

KW - TRANSPORT

KW - SEQUENCE

M3 - Article

VL - 182

SP - 6536

EP - 6540

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

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