Identification of an interferon antagonist protein encoded by segment 7 of infectious salmon anaemia virus

A J A McBeath, B Collet, R Paley, S Duraffour, V Aspehaug, E Biering, C J Secombes, M Snow

Research output: Contribution to journalArticle

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Abstract

Infectious salmon anaemia virus (ISAV) is an orthomyxovirus and member of the genus Isavirus, which contains eight genomic segments coding for ten viral proteins. This study focussed on identifying the function of the largest protein encoded by ISAV genomic segment 7 (7i), which like influenza A segment 7 encodes two proteins, one of which is based on removal of an intron from the primary transcript. Using two independent methods, an Mx1 promoter-driven reporter system and real-time PCR of FACS-sorted transfected cells, we demonstrate that the non-structural ISAV 7i protein is an interferon-signal ling antagonist. Other transfection studies indicated a predominantly cytoplasmic localisation of the expressed protein, which is consistent with this role. The demonstration that ISAV segment 7 encodes a putative non-structural IFN system antagonist reveals a difference with influenza A virus, where segment 7, which shares a similar coding strategy, encodes the structural matrix proteins. Crown Copyright (c) 2005 Published by Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)176-184
Number of pages9
JournalVirus Research
Volume115
DOIs
Publication statusPublished - 2006

Keywords

  • infectious salmon anaemia virus (ISAV)
  • interferon
  • Mx
  • segment 7
  • DOUBLE-STRANDED-RNA
  • NF-KAPPA-B
  • NS1 PROTEIN
  • INFLUENZA-VIRUS
  • RAINBOW-TROUT
  • V-PROTEIN
  • FUNCTIONAL-CHARACTERIZATION
  • ALPHA-INTERFERON
  • GENOMIC SEGMENT
  • MESSENGER-RNAS

Cite this

McBeath, A. J. A., Collet, B., Paley, R., Duraffour, S., Aspehaug, V., Biering, E., ... Snow, M. (2006). Identification of an interferon antagonist protein encoded by segment 7 of infectious salmon anaemia virus. Virus Research, 115, 176-184. https://doi.org/10.1016/j.virusres.2005.08.005

Identification of an interferon antagonist protein encoded by segment 7 of infectious salmon anaemia virus. / McBeath, A J A ; Collet, B ; Paley, R ; Duraffour, S ; Aspehaug, V ; Biering, E ; Secombes, C J ; Snow, M .

In: Virus Research, Vol. 115, 2006, p. 176-184.

Research output: Contribution to journalArticle

McBeath, A J A ; Collet, B ; Paley, R ; Duraffour, S ; Aspehaug, V ; Biering, E ; Secombes, C J ; Snow, M . / Identification of an interferon antagonist protein encoded by segment 7 of infectious salmon anaemia virus. In: Virus Research. 2006 ; Vol. 115. pp. 176-184.
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AB - Infectious salmon anaemia virus (ISAV) is an orthomyxovirus and member of the genus Isavirus, which contains eight genomic segments coding for ten viral proteins. This study focussed on identifying the function of the largest protein encoded by ISAV genomic segment 7 (7i), which like influenza A segment 7 encodes two proteins, one of which is based on removal of an intron from the primary transcript. Using two independent methods, an Mx1 promoter-driven reporter system and real-time PCR of FACS-sorted transfected cells, we demonstrate that the non-structural ISAV 7i protein is an interferon-signal ling antagonist. Other transfection studies indicated a predominantly cytoplasmic localisation of the expressed protein, which is consistent with this role. The demonstration that ISAV segment 7 encodes a putative non-structural IFN system antagonist reveals a difference with influenza A virus, where segment 7, which shares a similar coding strategy, encodes the structural matrix proteins. Crown Copyright (c) 2005 Published by Elsevier B.V. All rights reserved.

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