Abstract
Protein-bound pyrroles are a sign of oxidative damage. Here we report a specific method for detecting pyrrole-containing proteins using biotin-labeled Ehrlich's reagent (ER-B). After treatment of either human serum or isolated human serum proteins with various oxidizing agents, damaged, biotin-labeled components could be detected by blotting. Combining the use of ER-B with proteomic techniques allowed human serum proteins Susceptible to oxidative damage to be detected and then identified by LC/MS/MS. Identification of such proteins in different human conditions such as obesity, diabetes, and cardiovascular disease should lead to the discovery of new biomarkers and the development of specific assays to monitor health status.
| Original language | English |
|---|---|
| Pages (from-to) | 76-82 |
| Number of pages | 7 |
| Journal | Analytical Biochemistry |
| Volume | 398 |
| Issue number | 1 |
| Early online date | 20 Nov 2009 |
| DOIs | |
| Publication status | Published - 1 Mar 2010 |
Keywords
- protein oxidation
- protein-bound pyrroles
- modified Ehrlich's reagent
- detection of damaged proteins
- proteomics
- tail tendon collagen
- advanced glycation
- Maillard Reaction
- cross-linking
- oxidative stress
- diabetic-rats
- human plasma
- lysine
- 4-ketoaldehydes
- model
Access to Document
- Detecting damaged Proteins
NOTICE: this is the author’s version of a work that was accepted for publication Analytical Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in ANALYTICAL BIOCHEMISTRY, [VOL 398, ISSUE 1, (2010)] DOI: 10.1016/j.ab.2009.11.021