Identification of Hake species (Merluccius Genus) using sequencing and PCR-RFLP analysis of mitochondrial DNA control region sequences

J Quinteiro, R Vidal, M Izquierdo, C G Sotelo, M J Chapela, R I Pérez-Martín, H Rehbein, Georgina Louise Hold, Valerie Joan russell, Susan E Pryde, C Rosa, Ana T Santos, M Rey-Méndez

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The use of DNA-based methodologies in identification of hake species belonging to the Merluccius genus was shown to be successful. A short fragment of the left hypervariable domain of the mitochondrial control region was amplified, sequenced, and digested from 11 hake species. The hake-specific PCR product, due to its limited size, was obtained in a variety of tissue samples with different levels of DNA concentration and degradation, including sterilized food products. On the basis of this phylogenetically informative 156-bp sequence were selected four restriction enzymes (ApoI, DdeI, DraIII, and MboII) that allow the hake species discrimination. Species identification by phylogenetic analysis of sequences or by PCR-RFLP methodologies is useful in a variety of scenarios including authentication of thermally processed food, detection of food components, and species determination of individuals whose morphological characters are removed.
Original languageEnglish
Pages (from-to)5108-5114
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Issue number11
Publication statusPublished - 1 Nov 2001


  • Animals
  • Base Sequence
  • DNA, Mitochondrial
  • Fishes
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Sequence Homology, Nucleic Acid
  • Species Specificity

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