Abstract
The use of DNA-based methodologies in identification of hake species belonging to the Merluccius genus was shown to be successful. A short fragment of the left hypervariable domain of the mitochondrial control region was amplified, sequenced, and digested from 11 hake species. The hake-specific PCR product, due to its limited size, was obtained in a variety of tissue samples with different levels of DNA concentration and degradation, including sterilized food products. On the basis of this phylogenetically informative 156-bp sequence were selected four restriction enzymes (ApoI, DdeI, DraIII, and MboII) that allow the hake species discrimination. Species identification by phylogenetic analysis of sequences or by PCR-RFLP methodologies is useful in a variety of scenarios including authentication of thermally processed food, detection of food components, and species determination of individuals whose morphological characters are removed.
Original language | English |
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Pages (from-to) | 5108-5114 |
Number of pages | 7 |
Journal | Journal of Agricultural and Food Chemistry |
Volume | 49 |
Issue number | 11 |
DOIs | |
Publication status | Published - 1 Nov 2001 |
Keywords
- Animals
- Base Sequence
- DNA, Mitochondrial
- Fishes
- Molecular Sequence Data
- Phylogeny
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Sequence Homology, Nucleic Acid
- Species Specificity