Identification of Hake species (Merluccius Genus) using sequencing and PCR-RFLP analysis of mitochondrial DNA control region sequences

J Quinteiro, R Vidal, M Izquierdo, C G Sotelo, M J Chapela, R I Pérez-Martín, H Rehbein, Georgina Louise Hold, Valerie Joan russell, Susan E Pryde, C Rosa, Ana T Santos, M Rey-Méndez

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The use of DNA-based methodologies in identification of hake species belonging to the Merluccius genus was shown to be successful. A short fragment of the left hypervariable domain of the mitochondrial control region was amplified, sequenced, and digested from 11 hake species. The hake-specific PCR product, due to its limited size, was obtained in a variety of tissue samples with different levels of DNA concentration and degradation, including sterilized food products. On the basis of this phylogenetically informative 156-bp sequence were selected four restriction enzymes (ApoI, DdeI, DraIII, and MboII) that allow the hake species discrimination. Species identification by phylogenetic analysis of sequences or by PCR-RFLP methodologies is useful in a variety of scenarios including authentication of thermally processed food, detection of food components, and species determination of individuals whose morphological characters are removed.
Original languageEnglish
Pages (from-to)5108-5114
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume49
Issue number11
DOIs
Publication statusPublished - 1 Nov 2001

Keywords

  • Animals
  • Base Sequence
  • DNA, Mitochondrial
  • Fishes
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Sequence Homology, Nucleic Acid
  • Species Specificity

Cite this

Quinteiro, J., Vidal, R., Izquierdo, M., Sotelo, C. G., Chapela, M. J., Pérez-Martín, R. I., ... Rey-Méndez, M. (2001). Identification of Hake species (Merluccius Genus) using sequencing and PCR-RFLP analysis of mitochondrial DNA control region sequences. Journal of Agricultural and Food Chemistry, 49(11), 5108-5114. https://doi.org/10.1021/jf010421f

Identification of Hake species (Merluccius Genus) using sequencing and PCR-RFLP analysis of mitochondrial DNA control region sequences. / Quinteiro, J; Vidal, R; Izquierdo, M; Sotelo, C G; Chapela, M J; Pérez-Martín, R I; Rehbein, H; Hold, Georgina Louise; russell, Valerie Joan; Pryde, Susan E; Rosa, C; Santos, Ana T; Rey-Méndez, M.

In: Journal of Agricultural and Food Chemistry, Vol. 49, No. 11, 01.11.2001, p. 5108-5114.

Research output: Contribution to journalArticle

Quinteiro, J, Vidal, R, Izquierdo, M, Sotelo, CG, Chapela, MJ, Pérez-Martín, RI, Rehbein, H, Hold, GL, russell, VJ, Pryde, SE, Rosa, C, Santos, AT & Rey-Méndez, M 2001, 'Identification of Hake species (Merluccius Genus) using sequencing and PCR-RFLP analysis of mitochondrial DNA control region sequences', Journal of Agricultural and Food Chemistry, vol. 49, no. 11, pp. 5108-5114. https://doi.org/10.1021/jf010421f
Quinteiro, J ; Vidal, R ; Izquierdo, M ; Sotelo, C G ; Chapela, M J ; Pérez-Martín, R I ; Rehbein, H ; Hold, Georgina Louise ; russell, Valerie Joan ; Pryde, Susan E ; Rosa, C ; Santos, Ana T ; Rey-Méndez, M. / Identification of Hake species (Merluccius Genus) using sequencing and PCR-RFLP analysis of mitochondrial DNA control region sequences. In: Journal of Agricultural and Food Chemistry. 2001 ; Vol. 49, No. 11. pp. 5108-5114.
@article{42d869ae1a1b471f992d1aee15ba3b7d,
title = "Identification of Hake species (Merluccius Genus) using sequencing and PCR-RFLP analysis of mitochondrial DNA control region sequences",
abstract = "The use of DNA-based methodologies in identification of hake species belonging to the Merluccius genus was shown to be successful. A short fragment of the left hypervariable domain of the mitochondrial control region was amplified, sequenced, and digested from 11 hake species. The hake-specific PCR product, due to its limited size, was obtained in a variety of tissue samples with different levels of DNA concentration and degradation, including sterilized food products. On the basis of this phylogenetically informative 156-bp sequence were selected four restriction enzymes (ApoI, DdeI, DraIII, and MboII) that allow the hake species discrimination. Species identification by phylogenetic analysis of sequences or by PCR-RFLP methodologies is useful in a variety of scenarios including authentication of thermally processed food, detection of food components, and species determination of individuals whose morphological characters are removed.",
keywords = "Animals, Base Sequence, DNA, Mitochondrial, Fishes, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Sequence Homology, Nucleic Acid, Species Specificity",
author = "J Quinteiro and R Vidal and M Izquierdo and Sotelo, {C G} and Chapela, {M J} and P{\'e}rez-Mart{\'i}n, {R I} and H Rehbein and Hold, {Georgina Louise} and russell, {Valerie Joan} and Pryde, {Susan E} and C Rosa and Santos, {Ana T} and M Rey-M{\'e}ndez",
year = "2001",
month = "11",
day = "1",
doi = "10.1021/jf010421f",
language = "English",
volume = "49",
pages = "5108--5114",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "11",

}

TY - JOUR

T1 - Identification of Hake species (Merluccius Genus) using sequencing and PCR-RFLP analysis of mitochondrial DNA control region sequences

AU - Quinteiro, J

AU - Vidal, R

AU - Izquierdo, M

AU - Sotelo, C G

AU - Chapela, M J

AU - Pérez-Martín, R I

AU - Rehbein, H

AU - Hold, Georgina Louise

AU - russell, Valerie Joan

AU - Pryde, Susan E

AU - Rosa, C

AU - Santos, Ana T

AU - Rey-Méndez, M

PY - 2001/11/1

Y1 - 2001/11/1

N2 - The use of DNA-based methodologies in identification of hake species belonging to the Merluccius genus was shown to be successful. A short fragment of the left hypervariable domain of the mitochondrial control region was amplified, sequenced, and digested from 11 hake species. The hake-specific PCR product, due to its limited size, was obtained in a variety of tissue samples with different levels of DNA concentration and degradation, including sterilized food products. On the basis of this phylogenetically informative 156-bp sequence were selected four restriction enzymes (ApoI, DdeI, DraIII, and MboII) that allow the hake species discrimination. Species identification by phylogenetic analysis of sequences or by PCR-RFLP methodologies is useful in a variety of scenarios including authentication of thermally processed food, detection of food components, and species determination of individuals whose morphological characters are removed.

AB - The use of DNA-based methodologies in identification of hake species belonging to the Merluccius genus was shown to be successful. A short fragment of the left hypervariable domain of the mitochondrial control region was amplified, sequenced, and digested from 11 hake species. The hake-specific PCR product, due to its limited size, was obtained in a variety of tissue samples with different levels of DNA concentration and degradation, including sterilized food products. On the basis of this phylogenetically informative 156-bp sequence were selected four restriction enzymes (ApoI, DdeI, DraIII, and MboII) that allow the hake species discrimination. Species identification by phylogenetic analysis of sequences or by PCR-RFLP methodologies is useful in a variety of scenarios including authentication of thermally processed food, detection of food components, and species determination of individuals whose morphological characters are removed.

KW - Animals

KW - Base Sequence

KW - DNA, Mitochondrial

KW - Fishes

KW - Molecular Sequence Data

KW - Phylogeny

KW - Polymerase Chain Reaction

KW - Polymorphism, Restriction Fragment Length

KW - Sequence Homology, Nucleic Acid

KW - Species Specificity

U2 - 10.1021/jf010421f

DO - 10.1021/jf010421f

M3 - Article

VL - 49

SP - 5108

EP - 5114

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 11

ER -