Identification of Candida glabrata by a 30-second trehalase test

A. M. Freydiere, M. Crepy, A. Treny, H. Raberin, Amanda Denise Davidson, Frank Christopher Odds, F. Parant, F. Noel-Baron

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    Rapid (30-s) trehalase tests done with material from colonies of 482 yeasts suspended in a drop of trehalose solution on a commercially supplied glucose test strip were positive for 225 (99.1%) of 227 Candida glabrata isolates grown on either of two differential media, Candida ID medium or CandiSelect medium. The test was positive for only 3 (1.2%) and 12 (4.7%) of 255 isolates of other medically important yeast species grown on the same two media, respectively. A rapid maltase test done with a subset of 255 yeast isolates was negative for all but I of 64 trehalase-positive C. glabrata isolates, raising the specificity of the rapid testing for C glabrata to 98.4 to 100%, depending on the isolation medium used. Rapid trehalase and maltase tests done independently in two laboratories with 217 yeast isolates showed sensitivities of 96.0 to 98.0% and specificities of 98.2 to 99.4% for identification of C glabrata from colonies grown on Candida ID medium. The specificity was much lower because of frequent false-positive trehalose test results when the source of colonies was Sabouraud agar formulated with 4% glucose. We conclude that direct recognition of C. albicans as blue colonies on Candida ID isolation medium coupled with the performance of the 30-s trehalase and maltase tests for C. glabrata among the white colonies on this medium will allow the rapid presumptive identification of the two yeast species most commonly encountered in clinical samples.

    Original languageEnglish
    Pages (from-to)3602-3605
    Number of pages3
    JournalJournal of Clinical Microbiology
    Volume40
    Issue number10
    DOIs
    Publication statusPublished - 2002

    Keywords

    • BLOOD-STREAM INFECTION
    • RAPID IDENTIFICATION
    • CHROMAGAR CANDIDA
    • PRESUMPTIVE IDENTIFICATION
    • ANTIFUNGAL SUSCEPTIBILITY
    • NATIONAL SURVEILLANCE
    • CHROMOGENIC MEDIUM
    • SCOPE PROGRAM
    • ALBICANS
    • AGAR

    Cite this

    Freydiere, A. M., Crepy, M., Treny, A., Raberin, H., Davidson, A. D., Odds, F. C., ... Noel-Baron, F. (2002). Identification of Candida glabrata by a 30-second trehalase test. Journal of Clinical Microbiology, 40(10), 3602-3605. https://doi.org/10.1128/JCM.40.10.3602-3605.2002

    Identification of Candida glabrata by a 30-second trehalase test. / Freydiere, A. M.; Crepy, M.; Treny, A.; Raberin, H.; Davidson, Amanda Denise; Odds, Frank Christopher; Parant, F.; Noel-Baron, F.

    In: Journal of Clinical Microbiology, Vol. 40, No. 10, 2002, p. 3602-3605.

    Research output: Contribution to journalArticle

    Freydiere, AM, Crepy, M, Treny, A, Raberin, H, Davidson, AD, Odds, FC, Parant, F & Noel-Baron, F 2002, 'Identification of Candida glabrata by a 30-second trehalase test', Journal of Clinical Microbiology, vol. 40, no. 10, pp. 3602-3605. https://doi.org/10.1128/JCM.40.10.3602-3605.2002
    Freydiere AM, Crepy M, Treny A, Raberin H, Davidson AD, Odds FC et al. Identification of Candida glabrata by a 30-second trehalase test. Journal of Clinical Microbiology. 2002;40(10):3602-3605. https://doi.org/10.1128/JCM.40.10.3602-3605.2002
    Freydiere, A. M. ; Crepy, M. ; Treny, A. ; Raberin, H. ; Davidson, Amanda Denise ; Odds, Frank Christopher ; Parant, F. ; Noel-Baron, F. / Identification of Candida glabrata by a 30-second trehalase test. In: Journal of Clinical Microbiology. 2002 ; Vol. 40, No. 10. pp. 3602-3605.
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    abstract = "Rapid (30-s) trehalase tests done with material from colonies of 482 yeasts suspended in a drop of trehalose solution on a commercially supplied glucose test strip were positive for 225 (99.1{\%}) of 227 Candida glabrata isolates grown on either of two differential media, Candida ID medium or CandiSelect medium. The test was positive for only 3 (1.2{\%}) and 12 (4.7{\%}) of 255 isolates of other medically important yeast species grown on the same two media, respectively. A rapid maltase test done with a subset of 255 yeast isolates was negative for all but I of 64 trehalase-positive C. glabrata isolates, raising the specificity of the rapid testing for C glabrata to 98.4 to 100{\%}, depending on the isolation medium used. Rapid trehalase and maltase tests done independently in two laboratories with 217 yeast isolates showed sensitivities of 96.0 to 98.0{\%} and specificities of 98.2 to 99.4{\%} for identification of C glabrata from colonies grown on Candida ID medium. The specificity was much lower because of frequent false-positive trehalose test results when the source of colonies was Sabouraud agar formulated with 4{\%} glucose. We conclude that direct recognition of C. albicans as blue colonies on Candida ID isolation medium coupled with the performance of the 30-s trehalase and maltase tests for C. glabrata among the white colonies on this medium will allow the rapid presumptive identification of the two yeast species most commonly encountered in clinical samples.",
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    AU - Crepy, M.

    AU - Treny, A.

    AU - Raberin, H.

    AU - Davidson, Amanda Denise

    AU - Odds, Frank Christopher

    AU - Parant, F.

    AU - Noel-Baron, F.

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    N2 - Rapid (30-s) trehalase tests done with material from colonies of 482 yeasts suspended in a drop of trehalose solution on a commercially supplied glucose test strip were positive for 225 (99.1%) of 227 Candida glabrata isolates grown on either of two differential media, Candida ID medium or CandiSelect medium. The test was positive for only 3 (1.2%) and 12 (4.7%) of 255 isolates of other medically important yeast species grown on the same two media, respectively. A rapid maltase test done with a subset of 255 yeast isolates was negative for all but I of 64 trehalase-positive C. glabrata isolates, raising the specificity of the rapid testing for C glabrata to 98.4 to 100%, depending on the isolation medium used. Rapid trehalase and maltase tests done independently in two laboratories with 217 yeast isolates showed sensitivities of 96.0 to 98.0% and specificities of 98.2 to 99.4% for identification of C glabrata from colonies grown on Candida ID medium. The specificity was much lower because of frequent false-positive trehalose test results when the source of colonies was Sabouraud agar formulated with 4% glucose. We conclude that direct recognition of C. albicans as blue colonies on Candida ID isolation medium coupled with the performance of the 30-s trehalase and maltase tests for C. glabrata among the white colonies on this medium will allow the rapid presumptive identification of the two yeast species most commonly encountered in clinical samples.

    AB - Rapid (30-s) trehalase tests done with material from colonies of 482 yeasts suspended in a drop of trehalose solution on a commercially supplied glucose test strip were positive for 225 (99.1%) of 227 Candida glabrata isolates grown on either of two differential media, Candida ID medium or CandiSelect medium. The test was positive for only 3 (1.2%) and 12 (4.7%) of 255 isolates of other medically important yeast species grown on the same two media, respectively. A rapid maltase test done with a subset of 255 yeast isolates was negative for all but I of 64 trehalase-positive C. glabrata isolates, raising the specificity of the rapid testing for C glabrata to 98.4 to 100%, depending on the isolation medium used. Rapid trehalase and maltase tests done independently in two laboratories with 217 yeast isolates showed sensitivities of 96.0 to 98.0% and specificities of 98.2 to 99.4% for identification of C glabrata from colonies grown on Candida ID medium. The specificity was much lower because of frequent false-positive trehalose test results when the source of colonies was Sabouraud agar formulated with 4% glucose. We conclude that direct recognition of C. albicans as blue colonies on Candida ID isolation medium coupled with the performance of the 30-s trehalase and maltase tests for C. glabrata among the white colonies on this medium will allow the rapid presumptive identification of the two yeast species most commonly encountered in clinical samples.

    KW - BLOOD-STREAM INFECTION

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    KW - CHROMAGAR CANDIDA

    KW - PRESUMPTIVE IDENTIFICATION

    KW - ANTIFUNGAL SUSCEPTIBILITY

    KW - NATIONAL SURVEILLANCE

    KW - CHROMOGENIC MEDIUM

    KW - SCOPE PROGRAM

    KW - ALBICANS

    KW - AGAR

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    JO - Journal of Clinical Microbiology

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