Identification of methyl-branched fatty acids from the triacylglycerols of subcutaneous adipose tissue of lambs

A Smith, Alexander Graham Calder, A K Lough, W R H Duncan

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A concentrate of branched chain fatty acids (as methyl esters) was prepared from the triacylglycerols of subcutaneous adipose tissue lipids of lambs receiving a carbohydrate-rich (cereal diet). This was accomplished by procedures which allowed the removal of unsaturated components by peroxidation and straight chain saturated components by urea-adduct formation. The concentrate was analyzed by high resolution gas chromatography in combination with mass spectrometry and was shown to consist of a complex mixture of saturated methyl-substituted fatty acids. Methyl substitution occurred on even-numbered carbon atoms (relative to the carboxyl group) and the chain lengths of the acids ranged from 10 to 18 carbon atoms. Acids with one methyl substituent in the fatty acyl chain were most abundant; di-, tri- and tetramethyl-substituted acids were also present. The biosynthesis of these methyl-substituted acids is discussed briefly.
Original languageEnglish
Pages (from-to)953-960
Number of pages8
JournalLipids
Volume14
Issue number12
DOIs
Publication statusPublished - 1 Dec 1979

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Subcutaneous Fat
Triglycerides
Fatty Acids
Tissue
Acids
Carbon
Atoms
Biosynthesis
Nutrition
Complex Mixtures
Chain length
Gas chromatography
Gas Chromatography
Mass spectrometry
Urea
Mass Spectrometry
Esters
Substitution reactions
Carbohydrates
Diet

Keywords

  • Adipose Tissue
  • Animals
  • Fatty Acids
  • Gas Chromatography-Mass Spectrometry
  • Sheep
  • Triglycerides

Cite this

Identification of methyl-branched fatty acids from the triacylglycerols of subcutaneous adipose tissue of lambs. / Smith, A; Calder, Alexander Graham; Lough, A K; Duncan, W R H.

In: Lipids, Vol. 14, No. 12, 01.12.1979, p. 953-960.

Research output: Contribution to journalArticle

Smith, A ; Calder, Alexander Graham ; Lough, A K ; Duncan, W R H. / Identification of methyl-branched fatty acids from the triacylglycerols of subcutaneous adipose tissue of lambs. In: Lipids. 1979 ; Vol. 14, No. 12. pp. 953-960.
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