Abstract
xynB is one of at least four genes from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that encode xylanase activity. The xynB gene is predicted to encode a 781-amino acid product starting with a signal peptide, followed by an amino-terminal xylanase domain which is identical at 89% and 78% of residues, respectively, to the amino-terminal xylanase domains of the bifunctional XynD and XynA enzymes from the same organism. Two separate regions within the carboxy-terminal 537 amino acids of XynB also show close similarities with domain B of XynD. These regions show no significant homology with cellulose- or xylan-binding domains from other species, or with any other sequences, and their functions are unknown. In addition a 30 to 32-residue threonine-rich region is present in both XynD and XynB. Codon usage shows a consistent pattern of bias in the three xylanase genes from R. flavefaciens that have been sequenced.
Original language | English |
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Pages (from-to) | 260-4 |
Number of pages | 5 |
Journal | Molecular & general genetics |
Volume | 245 |
Issue number | 2 |
Publication status | Published - 28 Oct 1994 |
Keywords
- Amino Acid Sequence
- Animals
- Bacillus subtilis
- Bacteria, Anaerobic
- Bacterial Proteins
- Base Sequence
- Escherichia coli
- Genes, Bacterial
- Gram-Positive Cocci
- Molecular Sequence Data
- Rumen
- Sequence Alignment
- Sequence Homology, Amino Acid
- Species Specificity
- Xylan Endo-1,3-beta-Xylosidase
- Xylosidases