Identification of non-catalytic conserved regions in xylanases encoded by the xynB and xynD genes of the cellulolytic rumen anaerobe Ruminococcus flavefaciens

J X Zhang, J Martin, Harry James Flint, Jennifer Cynthia Martin

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

xynB is one of at least four genes from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that encode xylanase activity. The xynB gene is predicted to encode a 781-amino acid product starting with a signal peptide, followed by an amino-terminal xylanase domain which is identical at 89% and 78% of residues, respectively, to the amino-terminal xylanase domains of the bifunctional XynD and XynA enzymes from the same organism. Two separate regions within the carboxy-terminal 537 amino acids of XynB also show close similarities with domain B of XynD. These regions show no significant homology with cellulose- or xylan-binding domains from other species, or with any other sequences, and their functions are unknown. In addition a 30 to 32-residue threonine-rich region is present in both XynD and XynB. Codon usage shows a consistent pattern of bias in the three xylanase genes from R. flavefaciens that have been sequenced.

Original languageEnglish
Pages (from-to)260-4
Number of pages5
JournalMolecular & general genetics
Volume245
Issue number2
Publication statusPublished - 28 Oct 1994

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Ruminococcus
Rumen
vpr Genes
Amino Acids
Xylans
Threonine
Protein Sorting Signals
Codon
Cellulose
Genes
Enzymes
XynB xylanase

Keywords

  • Amino Acid Sequence
  • Animals
  • Bacillus subtilis
  • Bacteria, Anaerobic
  • Bacterial Proteins
  • Base Sequence
  • Escherichia coli
  • Genes, Bacterial
  • Gram-Positive Cocci
  • Molecular Sequence Data
  • Rumen
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Xylan Endo-1,3-beta-Xylosidase
  • Xylosidases

Cite this

Identification of non-catalytic conserved regions in xylanases encoded by the xynB and xynD genes of the cellulolytic rumen anaerobe Ruminococcus flavefaciens. / Zhang, J X; Martin, J; Flint, Harry James; Martin, Jennifer Cynthia.

In: Molecular & general genetics, Vol. 245, No. 2, 28.10.1994, p. 260-4.

Research output: Contribution to journalArticle

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title = "Identification of non-catalytic conserved regions in xylanases encoded by the xynB and xynD genes of the cellulolytic rumen anaerobe Ruminococcus flavefaciens",
abstract = "xynB is one of at least four genes from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that encode xylanase activity. The xynB gene is predicted to encode a 781-amino acid product starting with a signal peptide, followed by an amino-terminal xylanase domain which is identical at 89{\%} and 78{\%} of residues, respectively, to the amino-terminal xylanase domains of the bifunctional XynD and XynA enzymes from the same organism. Two separate regions within the carboxy-terminal 537 amino acids of XynB also show close similarities with domain B of XynD. These regions show no significant homology with cellulose- or xylan-binding domains from other species, or with any other sequences, and their functions are unknown. In addition a 30 to 32-residue threonine-rich region is present in both XynD and XynB. Codon usage shows a consistent pattern of bias in the three xylanase genes from R. flavefaciens that have been sequenced.",
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T1 - Identification of non-catalytic conserved regions in xylanases encoded by the xynB and xynD genes of the cellulolytic rumen anaerobe Ruminococcus flavefaciens

AU - Zhang, J X

AU - Martin, J

AU - Flint, Harry James

AU - Martin, Jennifer Cynthia

PY - 1994/10/28

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N2 - xynB is one of at least four genes from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that encode xylanase activity. The xynB gene is predicted to encode a 781-amino acid product starting with a signal peptide, followed by an amino-terminal xylanase domain which is identical at 89% and 78% of residues, respectively, to the amino-terminal xylanase domains of the bifunctional XynD and XynA enzymes from the same organism. Two separate regions within the carboxy-terminal 537 amino acids of XynB also show close similarities with domain B of XynD. These regions show no significant homology with cellulose- or xylan-binding domains from other species, or with any other sequences, and their functions are unknown. In addition a 30 to 32-residue threonine-rich region is present in both XynD and XynB. Codon usage shows a consistent pattern of bias in the three xylanase genes from R. flavefaciens that have been sequenced.

AB - xynB is one of at least four genes from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that encode xylanase activity. The xynB gene is predicted to encode a 781-amino acid product starting with a signal peptide, followed by an amino-terminal xylanase domain which is identical at 89% and 78% of residues, respectively, to the amino-terminal xylanase domains of the bifunctional XynD and XynA enzymes from the same organism. Two separate regions within the carboxy-terminal 537 amino acids of XynB also show close similarities with domain B of XynD. These regions show no significant homology with cellulose- or xylan-binding domains from other species, or with any other sequences, and their functions are unknown. In addition a 30 to 32-residue threonine-rich region is present in both XynD and XynB. Codon usage shows a consistent pattern of bias in the three xylanase genes from R. flavefaciens that have been sequenced.

KW - Amino Acid Sequence

KW - Animals

KW - Bacillus subtilis

KW - Bacteria, Anaerobic

KW - Bacterial Proteins

KW - Base Sequence

KW - Escherichia coli

KW - Genes, Bacterial

KW - Gram-Positive Cocci

KW - Molecular Sequence Data

KW - Rumen

KW - Sequence Alignment

KW - Sequence Homology, Amino Acid

KW - Species Specificity

KW - Xylan Endo-1,3-beta-Xylosidase

KW - Xylosidases

M3 - Article

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JO - Molecular & general genetics

JF - Molecular & general genetics

SN - 0026-8925

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ER -