IDENTIFICATION OF NONCATALYTIC CONSERVED REGIONS IN XYLANASES ENCODED BY THE XYNB AND XYND GENES OF THE CELLULOLYTIC RUMEN ANAEROBE RUMINOCOCCUS-FLAVEFACIENS

J X ZHANG, J MARTIN, H J FLINT

Research output: Contribution to journalComment/debate

28 Citations (Scopus)

Abstract

xynB is one of at least four genes from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that encode xylanase activity. The xynB gene is predicted to encode a 781-amino acid product starting with a signal peptide, followed by an amino-terminal xylanase domain which is identical at 89% and 78% of residues, respectively, to the amino-terminal xylanase domains of the bifunctional XynD and XynA enzymes from the same organism. Two separate regions within the carboxy-terminal 537 amino acids of XynB also show close similarities with domain B of XynD. These regions show no significant homology with cellulose- or xylan-binding domains from other species, or with any other sequences, and their functions are unknown. In addition a 30 to 32-residue threonine-rich region is present in both XynD and XynB. Codon usage shows a consistent pattern of bias in the three xylanase genes from R. flavefaciens that have been sequenced.

Original languageEnglish
Pages (from-to)260-264
Number of pages5
JournalMolecular & general genetics
Volume245
Issue number2
Publication statusPublished - 28 Oct 1994

Keywords

  • XYLANASE
  • CELLULOLYTIC BACTERIA
  • ANAEROBIC BACTERIA
  • RUMINOCOCCUS
  • SEQUENCE CONSERVATION
  • SEQUENCE
  • DOMAINS
  • ENZYME

Cite this

IDENTIFICATION OF NONCATALYTIC CONSERVED REGIONS IN XYLANASES ENCODED BY THE XYNB AND XYND GENES OF THE CELLULOLYTIC RUMEN ANAEROBE RUMINOCOCCUS-FLAVEFACIENS. / ZHANG, J X ; MARTIN, J ; FLINT, H J .

In: Molecular & general genetics, Vol. 245, No. 2, 28.10.1994, p. 260-264.

Research output: Contribution to journalComment/debate

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title = "IDENTIFICATION OF NONCATALYTIC CONSERVED REGIONS IN XYLANASES ENCODED BY THE XYNB AND XYND GENES OF THE CELLULOLYTIC RUMEN ANAEROBE RUMINOCOCCUS-FLAVEFACIENS",
abstract = "xynB is one of at least four genes from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that encode xylanase activity. The xynB gene is predicted to encode a 781-amino acid product starting with a signal peptide, followed by an amino-terminal xylanase domain which is identical at 89{\%} and 78{\%} of residues, respectively, to the amino-terminal xylanase domains of the bifunctional XynD and XynA enzymes from the same organism. Two separate regions within the carboxy-terminal 537 amino acids of XynB also show close similarities with domain B of XynD. These regions show no significant homology with cellulose- or xylan-binding domains from other species, or with any other sequences, and their functions are unknown. In addition a 30 to 32-residue threonine-rich region is present in both XynD and XynB. Codon usage shows a consistent pattern of bias in the three xylanase genes from R. flavefaciens that have been sequenced.",
keywords = "XYLANASE, CELLULOLYTIC BACTERIA, ANAEROBIC BACTERIA, RUMINOCOCCUS, SEQUENCE CONSERVATION, SEQUENCE, DOMAINS, ENZYME",
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T1 - IDENTIFICATION OF NONCATALYTIC CONSERVED REGIONS IN XYLANASES ENCODED BY THE XYNB AND XYND GENES OF THE CELLULOLYTIC RUMEN ANAEROBE RUMINOCOCCUS-FLAVEFACIENS

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N2 - xynB is one of at least four genes from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that encode xylanase activity. The xynB gene is predicted to encode a 781-amino acid product starting with a signal peptide, followed by an amino-terminal xylanase domain which is identical at 89% and 78% of residues, respectively, to the amino-terminal xylanase domains of the bifunctional XynD and XynA enzymes from the same organism. Two separate regions within the carboxy-terminal 537 amino acids of XynB also show close similarities with domain B of XynD. These regions show no significant homology with cellulose- or xylan-binding domains from other species, or with any other sequences, and their functions are unknown. In addition a 30 to 32-residue threonine-rich region is present in both XynD and XynB. Codon usage shows a consistent pattern of bias in the three xylanase genes from R. flavefaciens that have been sequenced.

AB - xynB is one of at least four genes from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that encode xylanase activity. The xynB gene is predicted to encode a 781-amino acid product starting with a signal peptide, followed by an amino-terminal xylanase domain which is identical at 89% and 78% of residues, respectively, to the amino-terminal xylanase domains of the bifunctional XynD and XynA enzymes from the same organism. Two separate regions within the carboxy-terminal 537 amino acids of XynB also show close similarities with domain B of XynD. These regions show no significant homology with cellulose- or xylan-binding domains from other species, or with any other sequences, and their functions are unknown. In addition a 30 to 32-residue threonine-rich region is present in both XynD and XynB. Codon usage shows a consistent pattern of bias in the three xylanase genes from R. flavefaciens that have been sequenced.

KW - XYLANASE

KW - CELLULOLYTIC BACTERIA

KW - ANAEROBIC BACTERIA

KW - RUMINOCOCCUS

KW - SEQUENCE CONSERVATION

KW - SEQUENCE

KW - DOMAINS

KW - ENZYME

M3 - Comment/debate

VL - 245

SP - 260

EP - 264

JO - Molecular & general genetics

JF - Molecular & general genetics

SN - 0026-8925

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