Identification of novel dendritic cell populations in normal mouse retina

Heping Xu, Rosemary Anne Dawson, John Vincent Forrester, Janet Mary Liversidge

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

PURPOSE. Whether tissue resident or infiltrating antigen-presenting cells (APCs) are involved in modulating immune responses in the retina and initiating inflammation is controversial. In this histologic study, the authors examine the retinas of mice strains with different susceptibility to experimental autoimmune uveoretinitis (EAU) for tissue resident APC.

METHODS. Retinal wholemounts from normal and inflamed eyes of B10R III, C57BL/6, BALB/c, and ABH Biozii mice were immunostained for APC markers (33D1, CD11c, CD11b, major histocompatibility complex [MHC] class II, F4/80, CD80, CD86, CD205, mPDCA, B220, and GR1) and analyzed by confocal fluorescence microscopy using emission fingerprinting and three-dimensional reconstruction techniques. Hematoxylin and eosin-stained histologic sections were used to evaluate EAU disease scores and to assess outer blood retina barrier (retinal pigment epithelium [RPE]) structures.

RESULTS. A population of 33D1(+) cells was identified exclusively in the peripheral margins and juxtapapillary areas of the retina in normal, nonimmunized C57BL/6 adult mice. These cells were also MHC class IIhigh, and their location corresponded to sites of earliest inflammation in EAU. Numbers in the papillary area were very low (less than 10), but this region marked the predominant anatomic site for initiation of inflammation in this moderately susceptible strain. The distribution and phenotype of these cells within the retinas differed between mouse strains exhibiting different disease susceptibility. In EAU-resistant BALB/c mice, many more 33D1(+) dendritic cells were present in the normal retina but were MHC class IIlow/-. Conversely, no 33D1(+) or MHC class II+ dendriform cells could be found in the normal retinas of highly EAU-susceptible B10. RIII mice.

CONCLUSIONS. A novel population of 33D1(+) DCs was identified in normal mouse retina. The function of these cells remains to be defined, but increased numbers correlate positively with structural abnormalities in the RPE and increased resistance of the strain to EAU.

Original languageEnglish
Pages (from-to)1701-1710
Number of pages10
JournalInvestigative Ophthalmology & Visual Science
Volume48
Issue number4
DOIs
Publication statusPublished - Apr 2007

Keywords

  • experimental autoimmune uveoretinitis
  • II-positive cells
  • in-vivo
  • immune privilege
  • parenchymal microglia
  • antigen presentation
  • anterior-chamber
  • lymph-nodes
  • uveal tract
  • IFN-gamma

Cite this

Identification of novel dendritic cell populations in normal mouse retina. / Xu, Heping; Dawson, Rosemary Anne; Forrester, John Vincent; Liversidge, Janet Mary.

In: Investigative Ophthalmology & Visual Science, Vol. 48, No. 4, 04.2007, p. 1701-1710.

Research output: Contribution to journalArticle

Xu, Heping ; Dawson, Rosemary Anne ; Forrester, John Vincent ; Liversidge, Janet Mary. / Identification of novel dendritic cell populations in normal mouse retina. In: Investigative Ophthalmology & Visual Science. 2007 ; Vol. 48, No. 4. pp. 1701-1710.
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title = "Identification of novel dendritic cell populations in normal mouse retina",
abstract = "PURPOSE. Whether tissue resident or infiltrating antigen-presenting cells (APCs) are involved in modulating immune responses in the retina and initiating inflammation is controversial. In this histologic study, the authors examine the retinas of mice strains with different susceptibility to experimental autoimmune uveoretinitis (EAU) for tissue resident APC.METHODS. Retinal wholemounts from normal and inflamed eyes of B10R III, C57BL/6, BALB/c, and ABH Biozii mice were immunostained for APC markers (33D1, CD11c, CD11b, major histocompatibility complex [MHC] class II, F4/80, CD80, CD86, CD205, mPDCA, B220, and GR1) and analyzed by confocal fluorescence microscopy using emission fingerprinting and three-dimensional reconstruction techniques. Hematoxylin and eosin-stained histologic sections were used to evaluate EAU disease scores and to assess outer blood retina barrier (retinal pigment epithelium [RPE]) structures.RESULTS. A population of 33D1(+) cells was identified exclusively in the peripheral margins and juxtapapillary areas of the retina in normal, nonimmunized C57BL/6 adult mice. These cells were also MHC class IIhigh, and their location corresponded to sites of earliest inflammation in EAU. Numbers in the papillary area were very low (less than 10), but this region marked the predominant anatomic site for initiation of inflammation in this moderately susceptible strain. The distribution and phenotype of these cells within the retinas differed between mouse strains exhibiting different disease susceptibility. In EAU-resistant BALB/c mice, many more 33D1(+) dendritic cells were present in the normal retina but were MHC class IIlow/-. Conversely, no 33D1(+) or MHC class II+ dendriform cells could be found in the normal retinas of highly EAU-susceptible B10. RIII mice.CONCLUSIONS. A novel population of 33D1(+) DCs was identified in normal mouse retina. The function of these cells remains to be defined, but increased numbers correlate positively with structural abnormalities in the RPE and increased resistance of the strain to EAU.",
keywords = "experimental autoimmune uveoretinitis, II-positive cells, in-vivo, immune privilege, parenchymal microglia, antigen presentation, anterior-chamber, lymph-nodes, uveal tract, IFN-gamma",
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T1 - Identification of novel dendritic cell populations in normal mouse retina

AU - Xu, Heping

AU - Dawson, Rosemary Anne

AU - Forrester, John Vincent

AU - Liversidge, Janet Mary

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N2 - PURPOSE. Whether tissue resident or infiltrating antigen-presenting cells (APCs) are involved in modulating immune responses in the retina and initiating inflammation is controversial. In this histologic study, the authors examine the retinas of mice strains with different susceptibility to experimental autoimmune uveoretinitis (EAU) for tissue resident APC.METHODS. Retinal wholemounts from normal and inflamed eyes of B10R III, C57BL/6, BALB/c, and ABH Biozii mice were immunostained for APC markers (33D1, CD11c, CD11b, major histocompatibility complex [MHC] class II, F4/80, CD80, CD86, CD205, mPDCA, B220, and GR1) and analyzed by confocal fluorescence microscopy using emission fingerprinting and three-dimensional reconstruction techniques. Hematoxylin and eosin-stained histologic sections were used to evaluate EAU disease scores and to assess outer blood retina barrier (retinal pigment epithelium [RPE]) structures.RESULTS. A population of 33D1(+) cells was identified exclusively in the peripheral margins and juxtapapillary areas of the retina in normal, nonimmunized C57BL/6 adult mice. These cells were also MHC class IIhigh, and their location corresponded to sites of earliest inflammation in EAU. Numbers in the papillary area were very low (less than 10), but this region marked the predominant anatomic site for initiation of inflammation in this moderately susceptible strain. The distribution and phenotype of these cells within the retinas differed between mouse strains exhibiting different disease susceptibility. In EAU-resistant BALB/c mice, many more 33D1(+) dendritic cells were present in the normal retina but were MHC class IIlow/-. Conversely, no 33D1(+) or MHC class II+ dendriform cells could be found in the normal retinas of highly EAU-susceptible B10. RIII mice.CONCLUSIONS. A novel population of 33D1(+) DCs was identified in normal mouse retina. The function of these cells remains to be defined, but increased numbers correlate positively with structural abnormalities in the RPE and increased resistance of the strain to EAU.

AB - PURPOSE. Whether tissue resident or infiltrating antigen-presenting cells (APCs) are involved in modulating immune responses in the retina and initiating inflammation is controversial. In this histologic study, the authors examine the retinas of mice strains with different susceptibility to experimental autoimmune uveoretinitis (EAU) for tissue resident APC.METHODS. Retinal wholemounts from normal and inflamed eyes of B10R III, C57BL/6, BALB/c, and ABH Biozii mice were immunostained for APC markers (33D1, CD11c, CD11b, major histocompatibility complex [MHC] class II, F4/80, CD80, CD86, CD205, mPDCA, B220, and GR1) and analyzed by confocal fluorescence microscopy using emission fingerprinting and three-dimensional reconstruction techniques. Hematoxylin and eosin-stained histologic sections were used to evaluate EAU disease scores and to assess outer blood retina barrier (retinal pigment epithelium [RPE]) structures.RESULTS. A population of 33D1(+) cells was identified exclusively in the peripheral margins and juxtapapillary areas of the retina in normal, nonimmunized C57BL/6 adult mice. These cells were also MHC class IIhigh, and their location corresponded to sites of earliest inflammation in EAU. Numbers in the papillary area were very low (less than 10), but this region marked the predominant anatomic site for initiation of inflammation in this moderately susceptible strain. The distribution and phenotype of these cells within the retinas differed between mouse strains exhibiting different disease susceptibility. In EAU-resistant BALB/c mice, many more 33D1(+) dendritic cells were present in the normal retina but were MHC class IIlow/-. Conversely, no 33D1(+) or MHC class II+ dendriform cells could be found in the normal retinas of highly EAU-susceptible B10. RIII mice.CONCLUSIONS. A novel population of 33D1(+) DCs was identified in normal mouse retina. The function of these cells remains to be defined, but increased numbers correlate positively with structural abnormalities in the RPE and increased resistance of the strain to EAU.

KW - experimental autoimmune uveoretinitis

KW - II-positive cells

KW - in-vivo

KW - immune privilege

KW - parenchymal microglia

KW - antigen presentation

KW - anterior-chamber

KW - lymph-nodes

KW - uveal tract

KW - IFN-gamma

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DO - 10.1167/iovs.06-0697

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JO - Investigative Ophthalmology & Visual Science

JF - Investigative Ophthalmology & Visual Science

SN - 0146-0404

IS - 4

ER -