Identification of suppressor of cytokine signalling (SOCS) 6, 7, 9 and CISH in rainbow trout Oncorhynchus mykiss and analysis of their expression in relation to other known trout SOCS

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Abstract

Four new members of the SOCS family of molecules in rainbow trout (Oncorhynchus mykiss), CISH and SOCS6, 7 and 9, are described for the first time in this species. The genes had a wide tissue distribution in trout, and were detected in gills, skin, muscle, liver, spleen, head kidney, intestine and brain, with brain having the highest expression levels. Stimulation of a rainbow trout leucocyte cell line, RTS-11, (mononuclear/macrophage-like cells) with LPS or Poly I:C had no effect on the expression of these genes, although in both cases the previously identified SOCS1-3 genes were up-regulated. Similarly, stimulation of RTS-11 or RTG-2 (fibroblasts) cells with the trout recombinant cytokines IFN-gamma or IL-1beta had no effect on CISH or SOCS6, 7 and 9 expression. However, PMA stimulation did impact on SOCS6 and SOCS9 expression, and LPS stimulation of primary cultures or bacterial infection (Yersinia ruckeri) increased significantly CISH expression (as well as SOCS1 and SOCS2 or SOCS3 respectively). It is apparent that the type II SOCS genes (CISH, SOCS1-3) are particularly relevant to immune regulation in fish, although the intriguing expansion of the SOCS4/5 subgroup in fish requires further investigation as to their role and functional divergence.
Original languageEnglish
Pages (from-to)656-667
Number of pages12
JournalFish & Shellfish Immunology
Volume29
Issue number4
Early online date19 Jun 2010
DOIs
Publication statusPublished - Oct 2010

Fingerprint

trout
rainbow
Oncorhynchus mykiss
cytokines
Genes
Cytokines
gene
Fish
Yersinia ruckeri
brain
polyinosinic-polycytidylic acid
Brain
genes
tissue distribution
bacterial infections
fish
skin (animal)
Poly I-C
fibroblasts
Macrophages

Keywords

  • adjuvants, immunologic
  • amino acid sequence
  • animals
  • cell line
  • cells, cultured
  • fish diseases
  • gene expression regulation
  • lipopolysaccharides
  • molecular sequence data
  • Oncorhynchus mykiss
  • sequence alignment
  • suppressor of cytokine signaling proteins
  • yersinia infections
  • yersinia ruckeri

Cite this

@article{7601e279cdb348398310ee90eb3b87b2,
title = "Identification of suppressor of cytokine signalling (SOCS) 6, 7, 9 and CISH in rainbow trout Oncorhynchus mykiss and analysis of their expression in relation to other known trout SOCS",
abstract = "Four new members of the SOCS family of molecules in rainbow trout (Oncorhynchus mykiss), CISH and SOCS6, 7 and 9, are described for the first time in this species. The genes had a wide tissue distribution in trout, and were detected in gills, skin, muscle, liver, spleen, head kidney, intestine and brain, with brain having the highest expression levels. Stimulation of a rainbow trout leucocyte cell line, RTS-11, (mononuclear/macrophage-like cells) with LPS or Poly I:C had no effect on the expression of these genes, although in both cases the previously identified SOCS1-3 genes were up-regulated. Similarly, stimulation of RTS-11 or RTG-2 (fibroblasts) cells with the trout recombinant cytokines IFN-gamma or IL-1beta had no effect on CISH or SOCS6, 7 and 9 expression. However, PMA stimulation did impact on SOCS6 and SOCS9 expression, and LPS stimulation of primary cultures or bacterial infection (Yersinia ruckeri) increased significantly CISH expression (as well as SOCS1 and SOCS2 or SOCS3 respectively). It is apparent that the type II SOCS genes (CISH, SOCS1-3) are particularly relevant to immune regulation in fish, although the intriguing expansion of the SOCS4/5 subgroup in fish requires further investigation as to their role and functional divergence.",
keywords = "adjuvants, immunologic, amino acid sequence, animals, cell line, cells, cultured, fish diseases, gene expression regulation, lipopolysaccharides, molecular sequence data, Oncorhynchus mykiss, sequence alignment, suppressor of cytokine signaling proteins, yersinia infections, yersinia ruckeri",
author = "Tiehui Wang and Qian Gao and Pin Nie and Secombes, {Christopher J}",
note = "Copyright 2010 Elsevier Ltd. All rights reserved.",
year = "2010",
month = "10",
doi = "10.1016/j.fsi.2010.06.015",
language = "English",
volume = "29",
pages = "656--667",
journal = "Fish & Shellfish Immunology",
issn = "1050-4648",
publisher = "ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD",
number = "4",

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TY - JOUR

T1 - Identification of suppressor of cytokine signalling (SOCS) 6, 7, 9 and CISH in rainbow trout Oncorhynchus mykiss and analysis of their expression in relation to other known trout SOCS

AU - Wang, Tiehui

AU - Gao, Qian

AU - Nie, Pin

AU - Secombes, Christopher J

N1 - Copyright 2010 Elsevier Ltd. All rights reserved.

PY - 2010/10

Y1 - 2010/10

N2 - Four new members of the SOCS family of molecules in rainbow trout (Oncorhynchus mykiss), CISH and SOCS6, 7 and 9, are described for the first time in this species. The genes had a wide tissue distribution in trout, and were detected in gills, skin, muscle, liver, spleen, head kidney, intestine and brain, with brain having the highest expression levels. Stimulation of a rainbow trout leucocyte cell line, RTS-11, (mononuclear/macrophage-like cells) with LPS or Poly I:C had no effect on the expression of these genes, although in both cases the previously identified SOCS1-3 genes were up-regulated. Similarly, stimulation of RTS-11 or RTG-2 (fibroblasts) cells with the trout recombinant cytokines IFN-gamma or IL-1beta had no effect on CISH or SOCS6, 7 and 9 expression. However, PMA stimulation did impact on SOCS6 and SOCS9 expression, and LPS stimulation of primary cultures or bacterial infection (Yersinia ruckeri) increased significantly CISH expression (as well as SOCS1 and SOCS2 or SOCS3 respectively). It is apparent that the type II SOCS genes (CISH, SOCS1-3) are particularly relevant to immune regulation in fish, although the intriguing expansion of the SOCS4/5 subgroup in fish requires further investigation as to their role and functional divergence.

AB - Four new members of the SOCS family of molecules in rainbow trout (Oncorhynchus mykiss), CISH and SOCS6, 7 and 9, are described for the first time in this species. The genes had a wide tissue distribution in trout, and were detected in gills, skin, muscle, liver, spleen, head kidney, intestine and brain, with brain having the highest expression levels. Stimulation of a rainbow trout leucocyte cell line, RTS-11, (mononuclear/macrophage-like cells) with LPS or Poly I:C had no effect on the expression of these genes, although in both cases the previously identified SOCS1-3 genes were up-regulated. Similarly, stimulation of RTS-11 or RTG-2 (fibroblasts) cells with the trout recombinant cytokines IFN-gamma or IL-1beta had no effect on CISH or SOCS6, 7 and 9 expression. However, PMA stimulation did impact on SOCS6 and SOCS9 expression, and LPS stimulation of primary cultures or bacterial infection (Yersinia ruckeri) increased significantly CISH expression (as well as SOCS1 and SOCS2 or SOCS3 respectively). It is apparent that the type II SOCS genes (CISH, SOCS1-3) are particularly relevant to immune regulation in fish, although the intriguing expansion of the SOCS4/5 subgroup in fish requires further investigation as to their role and functional divergence.

KW - adjuvants, immunologic

KW - amino acid sequence

KW - animals

KW - cell line

KW - cells, cultured

KW - fish diseases

KW - gene expression regulation

KW - lipopolysaccharides

KW - molecular sequence data

KW - Oncorhynchus mykiss

KW - sequence alignment

KW - suppressor of cytokine signaling proteins

KW - yersinia infections

KW - yersinia ruckeri

U2 - 10.1016/j.fsi.2010.06.015

DO - 10.1016/j.fsi.2010.06.015

M3 - Article

VL - 29

SP - 656

EP - 667

JO - Fish & Shellfish Immunology

JF - Fish & Shellfish Immunology

SN - 1050-4648

IS - 4

ER -