Identification of suppressor of cytokine signalling (SOCS) 6, 7, 9 and CISH in rainbow trout Oncorhynchus mykiss and analysis of their expression in relation to other known trout SOCS

Tiehui Wang, Qian Gao, Pin Nie, Christopher J Secombes

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Four new members of the SOCS family of molecules in rainbow trout (Oncorhynchus mykiss), CISH and SOCS6, 7 and 9, are described for the first time in this species. The genes had a wide tissue distribution in trout, and were detected in gills, skin, muscle, liver, spleen, head kidney, intestine and brain, with brain having the highest expression levels. Stimulation of a rainbow trout leucocyte cell line, RTS-11, (mononuclear/macrophage-like cells) with LPS or Poly I:C had no effect on the expression of these genes, although in both cases the previously identified SOCS1-3 genes were up-regulated. Similarly, stimulation of RTS-11 or RTG-2 (fibroblasts) cells with the trout recombinant cytokines IFN-gamma or IL-1beta had no effect on CISH or SOCS6, 7 and 9 expression. However, PMA stimulation did impact on SOCS6 and SOCS9 expression, and LPS stimulation of primary cultures or bacterial infection (Yersinia ruckeri) increased significantly CISH expression (as well as SOCS1 and SOCS2 or SOCS3 respectively). It is apparent that the type II SOCS genes (CISH, SOCS1-3) are particularly relevant to immune regulation in fish, although the intriguing expansion of the SOCS4/5 subgroup in fish requires further investigation as to their role and functional divergence.
Original languageEnglish
Pages (from-to)656-667
Number of pages12
JournalFish & Shellfish Immunology
Volume29
Issue number4
Early online date19 Jun 2010
DOIs
Publication statusPublished - Oct 2010

Keywords

  • adjuvants, immunologic
  • amino acid sequence
  • animals
  • cell line
  • cells, cultured
  • fish diseases
  • gene expression regulation
  • lipopolysaccharides
  • molecular sequence data
  • Oncorhynchus mykiss
  • sequence alignment
  • suppressor of cytokine signaling proteins
  • yersinia infections
  • yersinia ruckeri

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