Identification of the abiotic stress-related transcription in little Neptune grass Cymodocea nodosa with RNA-seq

E Malandrakis, O Dadali, M Kavouras, T Danis, P Panagiotaki, H Miliou, F C Kuepper, A Exadactylos

Research output: Contribution to journalArticle

1 Citation (Scopus)
6 Downloads (Pure)

Abstract

Seagrasses exhibit vital ecological roles in the marine environment. Nevertheless, the genomic resources available for seagrasses are still scarce. In the present study, the transcriptome of Cymodocea nodosa was sequenced with a view to study the molecular mechanisms underlying abiotic stress responses. The sequenced transcriptome for the species was near-complete and a high percentage of the transcripts was computationally annotated. An experimental simulation of marine plant exposure to extreme temperature (34°C), salinity (50psu) and their combination was conducted. A dynamic transcriptome 24h response (short-term) from stress initialization was recorded. The most noteworthy alteration in gene expression was observed in heat-stressed plants. Transcripts associated with development, photosynthesis, osmotic balance and stress-response were differentially expressed, under the set experimental conditions. Results indicate a potential negative interaction of heat and osmotic stress on seagrasses transcriptome.

Original languageEnglish
Pages (from-to)47-56
Number of pages10
JournalMarine Genomics
Volume34
Early online date4 Apr 2017
DOIs
Publication statusPublished - Aug 2017

Fingerprint

Neptune
Poaceae
Transcriptome
transcriptome
abiotic stress
RNA
transcription (genetics)
grass
grasses
stress response
Hot Temperature
Osmoregulation
gene expression
marine environment
Osmotic Pressure
Salinity
genomics
photosynthesis
Photosynthesis
osmotic stress

Keywords

  • Journal Article
  • Cymodocea nodosa
  • RNAseq
  • Transcriptome
  • Differential expression

Cite this

Malandrakis, E., Dadali, O., Kavouras, M., Danis, T., Panagiotaki, P., Miliou, H., ... Exadactylos, A. (2017). Identification of the abiotic stress-related transcription in little Neptune grass Cymodocea nodosa with RNA-seq. Marine Genomics, 34, 47-56. https://doi.org/10.1016/j.margen.2017.03.005

Identification of the abiotic stress-related transcription in little Neptune grass Cymodocea nodosa with RNA-seq. / Malandrakis, E; Dadali, O; Kavouras, M; Danis, T; Panagiotaki, P; Miliou, H; Kuepper, F C; Exadactylos, A.

In: Marine Genomics, Vol. 34, 08.2017, p. 47-56.

Research output: Contribution to journalArticle

Malandrakis, E, Dadali, O, Kavouras, M, Danis, T, Panagiotaki, P, Miliou, H, Kuepper, FC & Exadactylos, A 2017, 'Identification of the abiotic stress-related transcription in little Neptune grass Cymodocea nodosa with RNA-seq' Marine Genomics, vol. 34, pp. 47-56. https://doi.org/10.1016/j.margen.2017.03.005
Malandrakis, E ; Dadali, O ; Kavouras, M ; Danis, T ; Panagiotaki, P ; Miliou, H ; Kuepper, F C ; Exadactylos, A. / Identification of the abiotic stress-related transcription in little Neptune grass Cymodocea nodosa with RNA-seq. In: Marine Genomics. 2017 ; Vol. 34. pp. 47-56.
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abstract = "Seagrasses exhibit vital ecological roles in the marine environment. Nevertheless, the genomic resources available for seagrasses are still scarce. In the present study, the transcriptome of Cymodocea nodosa was sequenced with a view to study the molecular mechanisms underlying abiotic stress responses. The sequenced transcriptome for the species was near-complete and a high percentage of the transcripts was computationally annotated. An experimental simulation of marine plant exposure to extreme temperature (34°C), salinity (50psu) and their combination was conducted. A dynamic transcriptome 24h response (short-term) from stress initialization was recorded. The most noteworthy alteration in gene expression was observed in heat-stressed plants. Transcripts associated with development, photosynthesis, osmotic balance and stress-response were differentially expressed, under the set experimental conditions. Results indicate a potential negative interaction of heat and osmotic stress on seagrasses transcriptome.",
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