Identification of three IFN-γ inducible lysosomal thiol reductase (GILT)-like genes in mud crab Scylla paramamosain with distinct gene organizations and patterns of expression

Wen Shu Huang*, Li Peng Duan, Bei Huang, Li Hong Zhou, Ying Liang, Chen Ling Tu, Fang Fang Zhang, Pin Nie, Tiehui Wang

*Corresponding author for this work

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Vertebrate gamma-interferon inducible lysosomal thiol reductase (GILT) is an IFN-γ-inducible protein and is involved in MHCII-restricted antigen processing and cross-presentation of MHCI-restricted antigens in adaptive immunity. Outside of the endocytic MHC pathway, GILT regulates the cellular redox state, inhibits T cell activation, neutralizes extracellular pathogens and is also a host factor of some bacterial pathogens. In this report, we isolated and characterized three divergent GILT-like genes, GILT1, GILT2 and GILT3, which share only 30.9-40.4% identities in a crustacean mud crab Scylla paramamosain. Whilst the crab GILT1 and GILT3 possess four and five exons, respectively, the GILT2 is intronless, suggesting that GILT2 may arise from a recent retroposition event. The invertebrate GILT-like genes have diverse gene organizations and may be evolved in a species/lineage-specific manner as suggested by phylogenetic tree analysis. The amino acid sequences equivalent to human mature GILT are well conserved, including the GILT signature and nine of the ten cysteine residues that potentially form 5 disulfide bonds in human GILT, across the animal kingdom. However, most invertebrate GILT-like molecules lack the human-type N-terminal propeptide, as well as the human-type C-terminal with a conserved cysteine residue, suggesting differences in post translational processing and mode of action. All the three GILT-like genes are highly expressed in the hepatopancreas and up-regulated by pathogenic bacterial infection suggesting a role in immune defense against bacterial diseases. This study may provide the basis for further investigation of the expanding functions of GILT-like molecules in immunity and other physiological processes in mud crabs and other animals.

Original languageEnglish
Pages (from-to)78-88
Number of pages11
JournalGene
Volume570
Issue number1
Early online date4 Jun 2015
DOIs
Publication statusPublished - 1 Oct 2015

Fingerprint

Sulfhydryl Compounds
Interferon-gamma
Oxidoreductases
Genes
Invertebrates
Cysteine
Cross-Priming
Physiological Phenomena
Hepatopancreas
Antigen Presentation
Adaptive Immunity
Bacterial Infections
Disulfides
Oxidation-Reduction
Vertebrates
Amino Acid Sequence
Exons
Immunity
T-Lymphocytes
Antigens

Keywords

  • Cloning
  • Evolution
  • Expression
  • Gene organization
  • IFN-γ inducible lysosomal thiol reductase (GILT)
  • Mud crab Scylla paramamosain

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Identification of three IFN-γ inducible lysosomal thiol reductase (GILT)-like genes in mud crab Scylla paramamosain with distinct gene organizations and patterns of expression. / Huang, Wen Shu; Duan, Li Peng; Huang, Bei; Zhou, Li Hong; Liang, Ying; Tu, Chen Ling; Zhang, Fang Fang; Nie, Pin; Wang, Tiehui.

In: Gene, Vol. 570, No. 1, 01.10.2015, p. 78-88.

Research output: Contribution to journalArticle

Huang, Wen Shu ; Duan, Li Peng ; Huang, Bei ; Zhou, Li Hong ; Liang, Ying ; Tu, Chen Ling ; Zhang, Fang Fang ; Nie, Pin ; Wang, Tiehui. / Identification of three IFN-γ inducible lysosomal thiol reductase (GILT)-like genes in mud crab Scylla paramamosain with distinct gene organizations and patterns of expression. In: Gene. 2015 ; Vol. 570, No. 1. pp. 78-88.
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abstract = "Vertebrate gamma-interferon inducible lysosomal thiol reductase (GILT) is an IFN-γ-inducible protein and is involved in MHCII-restricted antigen processing and cross-presentation of MHCI-restricted antigens in adaptive immunity. Outside of the endocytic MHC pathway, GILT regulates the cellular redox state, inhibits T cell activation, neutralizes extracellular pathogens and is also a host factor of some bacterial pathogens. In this report, we isolated and characterized three divergent GILT-like genes, GILT1, GILT2 and GILT3, which share only 30.9-40.4{\%} identities in a crustacean mud crab Scylla paramamosain. Whilst the crab GILT1 and GILT3 possess four and five exons, respectively, the GILT2 is intronless, suggesting that GILT2 may arise from a recent retroposition event. The invertebrate GILT-like genes have diverse gene organizations and may be evolved in a species/lineage-specific manner as suggested by phylogenetic tree analysis. The amino acid sequences equivalent to human mature GILT are well conserved, including the GILT signature and nine of the ten cysteine residues that potentially form 5 disulfide bonds in human GILT, across the animal kingdom. However, most invertebrate GILT-like molecules lack the human-type N-terminal propeptide, as well as the human-type C-terminal with a conserved cysteine residue, suggesting differences in post translational processing and mode of action. All the three GILT-like genes are highly expressed in the hepatopancreas and up-regulated by pathogenic bacterial infection suggesting a role in immune defense against bacterial diseases. This study may provide the basis for further investigation of the expanding functions of GILT-like molecules in immunity and other physiological processes in mud crabs and other animals.",
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