Fonsecaea pedrosoi-induced Th17-cell differentiation in mice is fostered by Dectin-2 and suppressed by Mincle recognition

Marcel Wüthrich, Huafeng Wang, Mengyi Li, Tassanee Lerksuthirat, Sarah E Hardison, Gordon D Brown, Bruce Klein

Research output: Contribution to journalArticle

29 Citations (Scopus)
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Abstract

Chromoblastomycosis is a chronic skin infection caused by the pigmented saprophytic mould Fonsecaea pedrosoi. Chronicity of infection can be broken by a coordinated innate recognition of the spores by pattern recognition receptors. While Mincle signaling via the Syk/Card9 pathway is required for fungal recognition by host cells, it is not sufficient for host control. Exogenously applied TLR agonists are necessary to promote the induction of proinflammatory cytokines and clearance of infection in vivo. Here, we investigated whether costimulation by TLR agonists fosters the development of adaptive immune responses, by examining the development of fungus-specific T cells. Subcutaneous infection of mice with F. pedrosoi spores induced the activation, expansion, and differentiation of Ag-specific CD4(+) T cells but TLR costimulation did not further augment these T-cell responses. The Dectin-2/FcRγ/Card9 signaling pathway promoted the differentiation of fungus-specific CD4(+) T cells into Th17 cells, whereas Mincle inhibited the development of this T-helper subset in infected mice. These results indicate differential roles for Dectin-2 and Mincle in the generation of adaptive immune responses to F. pedrosoi infection.

Original languageEnglish
Pages (from-to)2542-2552
Number of pages11
JournalEuropean Journal of Immunology
Volume45
Issue number9
Early online date28 Jul 2015
DOIs
Publication statusPublished - Sep 2015

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Th17 Cells
Cell Differentiation
T-Lymphocytes
Infection
Fungi
Adaptive Immunity
Spores
Chromoblastomycosis
Pattern Recognition Receptors
mouse dectin-2
Cytokines
Skin

Keywords

  • Adaptive Immunity
  • Animals
  • CARD Signaling Adaptor Proteins
  • Cell Differentiation
  • Chromoblastomycosis
  • Gene Expression Regulation
  • Host-Pathogen Interactions
  • Injections, Subcutaneous
  • Intracellular Signaling Peptides and Proteins
  • Lectins, C-Type
  • Membrane Proteins
  • Mice
  • Mice, Transgenic
  • Protein-Tyrosine Kinases
  • Receptors, IgG
  • Saccharomycetales
  • Signal Transduction
  • Skin
  • Spores, Fungal
  • Th17 Cells

Cite this

Fonsecaea pedrosoi-induced Th17-cell differentiation in mice is fostered by Dectin-2 and suppressed by Mincle recognition. / Wüthrich, Marcel; Wang, Huafeng; Li, Mengyi; Lerksuthirat, Tassanee; Hardison, Sarah E; Brown, Gordon D; Klein, Bruce.

In: European Journal of Immunology, Vol. 45, No. 9, 09.2015, p. 2542-2552.

Research output: Contribution to journalArticle

Wüthrich, Marcel ; Wang, Huafeng ; Li, Mengyi ; Lerksuthirat, Tassanee ; Hardison, Sarah E ; Brown, Gordon D ; Klein, Bruce. / Fonsecaea pedrosoi-induced Th17-cell differentiation in mice is fostered by Dectin-2 and suppressed by Mincle recognition. In: European Journal of Immunology. 2015 ; Vol. 45, No. 9. pp. 2542-2552.
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abstract = "Chromoblastomycosis is a chronic skin infection caused by the pigmented saprophytic mould Fonsecaea pedrosoi. Chronicity of infection can be broken by a coordinated innate recognition of the spores by pattern recognition receptors. While Mincle signaling via the Syk/Card9 pathway is required for fungal recognition by host cells, it is not sufficient for host control. Exogenously applied TLR agonists are necessary to promote the induction of proinflammatory cytokines and clearance of infection in vivo. Here, we investigated whether costimulation by TLR agonists fosters the development of adaptive immune responses, by examining the development of fungus-specific T cells. Subcutaneous infection of mice with F. pedrosoi spores induced the activation, expansion, and differentiation of Ag-specific CD4(+) T cells but TLR costimulation did not further augment these T-cell responses. The Dectin-2/FcRγ/Card9 signaling pathway promoted the differentiation of fungus-specific CD4(+) T cells into Th17 cells, whereas Mincle inhibited the development of this T-helper subset in infected mice. These results indicate differential roles for Dectin-2 and Mincle in the generation of adaptive immune responses to F. pedrosoi infection.",
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AB - Chromoblastomycosis is a chronic skin infection caused by the pigmented saprophytic mould Fonsecaea pedrosoi. Chronicity of infection can be broken by a coordinated innate recognition of the spores by pattern recognition receptors. While Mincle signaling via the Syk/Card9 pathway is required for fungal recognition by host cells, it is not sufficient for host control. Exogenously applied TLR agonists are necessary to promote the induction of proinflammatory cytokines and clearance of infection in vivo. Here, we investigated whether costimulation by TLR agonists fosters the development of adaptive immune responses, by examining the development of fungus-specific T cells. Subcutaneous infection of mice with F. pedrosoi spores induced the activation, expansion, and differentiation of Ag-specific CD4(+) T cells but TLR costimulation did not further augment these T-cell responses. The Dectin-2/FcRγ/Card9 signaling pathway promoted the differentiation of fungus-specific CD4(+) T cells into Th17 cells, whereas Mincle inhibited the development of this T-helper subset in infected mice. These results indicate differential roles for Dectin-2 and Mincle in the generation of adaptive immune responses to F. pedrosoi infection.

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