Galleria melonella as an experimental in vivo host model for the fish-pathogenic oomycete Saprolegnia parasitica

Andreas Wuensch, Franziska Trusch, Nurul A Iberahim, Pieter Van West

Research output: Contribution to journalArticle

5 Citations (Scopus)
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Abstract

Oomycetes are eukaryotic pathogens infecting animals and plants. Amongst them Saprolegnia parasitica is a fish pathogenic oomycete causing devastating losses in the aquaculture industry. To secure fish supply, new drugs are in high demand and since fish experiments are time consuming, expensive and involve animal welfare issues the search for adequate model systems is essential. Galleria mellonella serves as a heterologous host model for bacterial and fungal infections. This study extends the use of G. mellonella for studying infections with oomycetes. Saprolegniales are highly pathogenic to the insects while in contrast, the plant pathogen Phytophthora infestans showed no pathogenicity. Melanisation of hyphae below the cuticle allowed direct macroscopic monitoring of disease progression. However, the melanin response is not systemic as for other pathogens but instead is very local. The mortality of the larvae is dose-dependent and can be induced by cysts or regenerating protoplasts as an alternative source of inoculation.
Original languageEnglish
Pages (from-to)182-189
Number of pages8
JournalFungal Biology
Volume122
Issue number2-3
Early online date16 Feb 2018
DOIs
Publication statusPublished - Feb 2018

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Saprolegnia
Saprolegnia parasitica
Oomycetes
Galleria
Fishes
Galleria mellonella
pathogen
plant pathogens
Saprolegniales
fish
Phytophthora infestans
animal pathogens
animal welfare
melanin
Animal Welfare
Aquaculture
melanization
aquaculture industry
Hyphae
Mycoses

Keywords

  • Galleria mellonella
  • host model
  • oomycetes
  • Saprolegnia parasitica

Cite this

Galleria melonella as an experimental in vivo host model for the fish-pathogenic oomycete Saprolegnia parasitica. / Wuensch, Andreas; Trusch, Franziska; Iberahim, Nurul A; Van West, Pieter.

In: Fungal Biology, Vol. 122, No. 2-3, 02.2018, p. 182-189.

Research output: Contribution to journalArticle

Wuensch, Andreas ; Trusch, Franziska ; Iberahim, Nurul A ; Van West, Pieter. / Galleria melonella as an experimental in vivo host model for the fish-pathogenic oomycete Saprolegnia parasitica. In: Fungal Biology. 2018 ; Vol. 122, No. 2-3. pp. 182-189.
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