In vitro evidence of root colonization suggests ecological versatility in the genus Mycena

Ella Thoen* (Corresponding Author), Christoffer Bugge Harder, Havard Kauserud, Synnøve S Botnen, Unni Vik, Andy F S Taylor, Audrius Menkis, Inger Skrede

*Corresponding author for this work

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Abstract

The root-associated habit has evolved on numerous occasions in different fungal lineages, suggesting a strong evolutionary pressure for saprotrophic fungi to switch to symbiotic associations with plants. Species within the ubiquitous, saprotrophic genus Mycena are frequently major components in molecular studies of root-associated fungal communities, suggesting that an evaluation of their trophic status is warranted. Here, we report on interactions between a range of Mycena species and the plant Betula pendula. Seventeen Mycena species were inoculated onto B. pendula seedlings. Physical interactions between hyphae and fine roots were examined using differential staining and fluorescence microscopy. Physiological interactions were investigated using 14 C and 32 P to show potential transfer between symbionts. All Mycena species associated closely with fine roots, showing hyphal penetration into the roots, which in some cases were intracellular. Seven species formed mantle-like structures around root tips, but none formed a Hartig net. Mycena pura and M. galopus both enhanced seedling growth, with M. pura showing significant transfer of 32 P to the seedlings. Our results support the view that several Mycena species can associate closely with plant roots and some may potentially occupy a transitional state between saprotrophy and biotrophy.

Original languageEnglish
Pages (from-to)601-612
Number of pages12
JournalNew Phytologist
Volume227
Issue number2
Early online date13 Apr 2020
DOIs
Publication statusPublished - Jul 2020

Keywords

  • biotrophy-saprotrophy continuum
  • ecological niches
  • functional diversity
  • mycena
  • root-associations
  • biotrophy–saprotrophy continuum
  • Mycena
  • HOST-SPECIFICITY
  • TREE ROOTS
  • DECOMPOSITION
  • MYCELIUM
  • MYCORRHIZAL FUNGI
  • ECTOMYCORRHIZAL FUNGI
  • PHYLOGENETIC-RELATIONSHIPS
  • LITTER
  • FUNGAL COMMUNITIES
  • SAPROTROPHIC FUNGI

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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    Thoen, E., Harder, C. B., Kauserud, H., Botnen, S. S., Vik, U., Taylor, A. F. S., Menkis, A., & Skrede, I. (2020). In vitro evidence of root colonization suggests ecological versatility in the genus Mycena. New Phytologist, 227(2), 601-612. https://doi.org/10.1111/nph.16545