In Vivo Application of Proton-Electron Double-Resonance Imaging

Shun Kishimoto, Murali C. Krishna, Valery V. Khramtsov, Hideo Utsumi, David J Lurie (Corresponding Author)

Research output: Contribution to journalReview article

11 Citations (Scopus)
5 Downloads (Pure)

Abstract

Significance:
Proton-electron double resonance imaging (PEDRI) employs electron paramagnetic resonance irradiation with low field magnetic resonance imaging (MRI) so that the electron spin polarization is transferred to nearby protons, resulting in higher signal. PEDRI provides information about free radical distribution and, indirectly, about the local microenvironment such as pO2, tissue permeability, redox status, and acid-base balance.
Recent Advances:
Local acid-base balance can be imaged by exploiting the different resonance frequency of radical probes between R and RH+ forms. Redox status can also be imaged using the loss of radical-related signal after reduction. These methods require optimized radical probes and pulse sequences.
Critical Issues:
High power radiofrequency irradiation is needed for optimum signal enhancement, which may be harmful to living tissue by unwanted heat deposition. Free radical probes differ depending on the purpose of PEDRI. Some probes are less effective for enhancing signal than others, which can reduce image quality. It is so far not possible to image endogenous radicals by PEDRI because low concentrations and broad line widths of the radicals lead to negligible signal enhancement.
Future Directions:
PEDRI has similarities with electron paramagnetic resonance imaging (EPRI) because both otechniques bserve the EPR signal; directly in the case of EPRI and indirectly with PEDRI. PEDRI provides information vital to research on homeostasis, development of diseases or treatment responses in vivo. It is expected that the development of new EPR techniques will give insights into novel PEDRI applications and vice versa.
Original languageEnglish
Pages (from-to)1345-1364
Number of pages20
JournalAntioxidants & Redox Signaling
Volume28
Issue number15
Early online date13 Nov 2017
DOIs
Publication statusPublished - 20 May 2018

Fingerprint

Protons
Electrons
Imaging techniques
Paramagnetic resonance
Electron Spin Resonance Spectroscopy
Acid-Base Equilibrium
Oxidation-Reduction
Free Radicals
Irradiation
Tissue
Spin polarization
Magnetic resonance
Permeability
Homeostasis
Linewidth
Hot Temperature
Image quality
Magnetic Resonance Imaging
Research

Keywords

  • Overhauser MRI
  • OMRI
  • PEDRI
  • free radical
  • imaging

Cite this

In Vivo Application of Proton-Electron Double-Resonance Imaging. / Kishimoto, Shun ; Krishna, Murali C.; Khramtsov, Valery V. ; Utsumi, Hideo ; Lurie, David J (Corresponding Author).

In: Antioxidants & Redox Signaling, Vol. 28, No. 15, 20.05.2018, p. 1345-1364.

Research output: Contribution to journalReview article

Kishimoto, S, Krishna, MC, Khramtsov, VV, Utsumi, H & Lurie, DJ 2018, 'In Vivo Application of Proton-Electron Double-Resonance Imaging', Antioxidants & Redox Signaling, vol. 28, no. 15, pp. 1345-1364. https://doi.org/10.1089/ars.2017.7341
Kishimoto, Shun ; Krishna, Murali C. ; Khramtsov, Valery V. ; Utsumi, Hideo ; Lurie, David J. / In Vivo Application of Proton-Electron Double-Resonance Imaging. In: Antioxidants & Redox Signaling. 2018 ; Vol. 28, No. 15. pp. 1345-1364.
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abstract = "Significance: Proton-electron double resonance imaging (PEDRI) employs electron paramagnetic resonance irradiation with low field magnetic resonance imaging (MRI) so that the electron spin polarization is transferred to nearby protons, resulting in higher signal. PEDRI provides information about free radical distribution and, indirectly, about the local microenvironment such as pO2, tissue permeability, redox status, and acid-base balance. Recent Advances: Local acid-base balance can be imaged by exploiting the different resonance frequency of radical probes between R and RH+ forms. Redox status can also be imaged using the loss of radical-related signal after reduction. These methods require optimized radical probes and pulse sequences. Critical Issues: High power radiofrequency irradiation is needed for optimum signal enhancement, which may be harmful to living tissue by unwanted heat deposition. Free radical probes differ depending on the purpose of PEDRI. Some probes are less effective for enhancing signal than others, which can reduce image quality. It is so far not possible to image endogenous radicals by PEDRI because low concentrations and broad line widths of the radicals lead to negligible signal enhancement. Future Directions: PEDRI has similarities with electron paramagnetic resonance imaging (EPRI) because both otechniques bserve the EPR signal; directly in the case of EPRI and indirectly with PEDRI. PEDRI provides information vital to research on homeostasis, development of diseases or treatment responses in vivo. It is expected that the development of new EPR techniques will give insights into novel PEDRI applications and vice versa.",
keywords = "Overhauser MRI, OMRI, PEDRI, free radical, imaging",
author = "Shun Kishimoto and Krishna, {Murali C.} and Khramtsov, {Valery V.} and Hideo Utsumi and Lurie, {David J}",
note = "This work was partially supported by NIH grants 1ZIABC010477-14 (MKC), CA194013 (VVK), CA192064 (VVK), U54GM104942 (VVK); by KAKENHI grant 16H05113 (H.U.) from the Japan Society for the Promotion of Science (HU) and start-up grant from the WVCTSI (VVK).",
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AU - Kishimoto, Shun

AU - Krishna, Murali C.

AU - Khramtsov, Valery V.

AU - Utsumi, Hideo

AU - Lurie, David J

N1 - This work was partially supported by NIH grants 1ZIABC010477-14 (MKC), CA194013 (VVK), CA192064 (VVK), U54GM104942 (VVK); by KAKENHI grant 16H05113 (H.U.) from the Japan Society for the Promotion of Science (HU) and start-up grant from the WVCTSI (VVK).

PY - 2018/5/20

Y1 - 2018/5/20

N2 - Significance: Proton-electron double resonance imaging (PEDRI) employs electron paramagnetic resonance irradiation with low field magnetic resonance imaging (MRI) so that the electron spin polarization is transferred to nearby protons, resulting in higher signal. PEDRI provides information about free radical distribution and, indirectly, about the local microenvironment such as pO2, tissue permeability, redox status, and acid-base balance. Recent Advances: Local acid-base balance can be imaged by exploiting the different resonance frequency of radical probes between R and RH+ forms. Redox status can also be imaged using the loss of radical-related signal after reduction. These methods require optimized radical probes and pulse sequences. Critical Issues: High power radiofrequency irradiation is needed for optimum signal enhancement, which may be harmful to living tissue by unwanted heat deposition. Free radical probes differ depending on the purpose of PEDRI. Some probes are less effective for enhancing signal than others, which can reduce image quality. It is so far not possible to image endogenous radicals by PEDRI because low concentrations and broad line widths of the radicals lead to negligible signal enhancement. Future Directions: PEDRI has similarities with electron paramagnetic resonance imaging (EPRI) because both otechniques bserve the EPR signal; directly in the case of EPRI and indirectly with PEDRI. PEDRI provides information vital to research on homeostasis, development of diseases or treatment responses in vivo. It is expected that the development of new EPR techniques will give insights into novel PEDRI applications and vice versa.

AB - Significance: Proton-electron double resonance imaging (PEDRI) employs electron paramagnetic resonance irradiation with low field magnetic resonance imaging (MRI) so that the electron spin polarization is transferred to nearby protons, resulting in higher signal. PEDRI provides information about free radical distribution and, indirectly, about the local microenvironment such as pO2, tissue permeability, redox status, and acid-base balance. Recent Advances: Local acid-base balance can be imaged by exploiting the different resonance frequency of radical probes between R and RH+ forms. Redox status can also be imaged using the loss of radical-related signal after reduction. These methods require optimized radical probes and pulse sequences. Critical Issues: High power radiofrequency irradiation is needed for optimum signal enhancement, which may be harmful to living tissue by unwanted heat deposition. Free radical probes differ depending on the purpose of PEDRI. Some probes are less effective for enhancing signal than others, which can reduce image quality. It is so far not possible to image endogenous radicals by PEDRI because low concentrations and broad line widths of the radicals lead to negligible signal enhancement. Future Directions: PEDRI has similarities with electron paramagnetic resonance imaging (EPRI) because both otechniques bserve the EPR signal; directly in the case of EPRI and indirectly with PEDRI. PEDRI provides information vital to research on homeostasis, development of diseases or treatment responses in vivo. It is expected that the development of new EPR techniques will give insights into novel PEDRI applications and vice versa.

KW - Overhauser MRI

KW - OMRI

KW - PEDRI

KW - free radical

KW - imaging

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DO - 10.1089/ars.2017.7341

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VL - 28

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JO - Antioxidants & Redox Signaling

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SN - 1523-0864

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ER -