IL-22 and TNF-a represent a key cytokine combination for epidermal integrity during infection with Candida albicans

Stefanie Eyerich (Corresponding Author), Jeanette Wagener, Vera Wenzel, Claudia Scarponi, Davide Pennino, Cristina Albanesi, Martin Schaller, Heidrun Behrendt, Johannes Ring, Carsten B Schmidt-Weber, Andrea Cavani, Martin Mempel, Claudia Traidl-Hoffmann, Kilian Eyerich

Research output: Contribution to journalArticle

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Abstract

T cells exercise their full impact on target cells through a combination of secreted cytokines. The recently described T helper cell subset Th22 is characterized by a combinatorial secretion of IL-22 and TNF-a. Here, we demonstrate that IL-22 increases the TNF-a-dependent induction and secretion of several immune-modulatory molecules such as initial complement factors C1r and C1s, antimicrobial peptides S100A7 and HBD-2 (human ß defensin 2), and antimicrobial chemokines CXCL-9/-10/-11 in primary human keratinocytes. The synergism of IL-22 and TNF-a is transmitted intracellularly by MAP kinases and downstream by transcription factors of the AP-1 family. The induction of innate immunity is relevant in an in vitro infection model, where keratinocytes stimulated with Th22 supernatants or recombinant IL-22 plus TNF-a effectively inhibit the growth of Candida albicans and maintain survival of epithelia. Accordingly, the combinatorial stimulation of keratinocytes with IL-22 and TNF-a most efficiently conserves the integrity of the epidermal barrier in a three-dimensional skin infection model as compared with IFN-¿, IL-17, IL-22 or TNF-a alone. In summary, we demonstrate that IL-22 and TNF-a represent a potent, synergistic cytokine combination for cutaneous immunity.
Original languageEnglish
Pages (from-to)1894-1901
Number of pages8
JournalEuropean Journal of Immunology
Volume41
Issue number7
Early online date20 May 2011
DOIs
Publication statusPublished - Jul 2011

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Candida albicans
Cytokines
Infection
Keratinocytes
Complement C1r
Complement C1s
Defensins
Skin
Interleukin-17
Transcription Factor AP-1
T-Lymphocyte Subsets
interleukin-22
Chemokines
Innate Immunity
Immunity
Phosphotransferases
Epithelium
T-Lymphocytes
Peptides
Growth

Keywords

  • Candida albicans
  • Candidiasis, Cutaneous
  • Chemokines
  • Complement C1r
  • Complement C1s
  • Enzyme-Linked Immunosorbent Assay
  • Epidermis
  • Humans
  • Immunity, Innate
  • Immunoblotting
  • Interleukins
  • Keratinocytes
  • Male
  • Mitogen-Activated Protein Kinases
  • Polymerase Chain Reaction
  • S100 Proteins
  • T-Lymphocytes, Helper-Inducer
  • Transcription Factor AP-1
  • Tumor Necrosis Factor-alpha
  • beta-Defensins

Cite this

IL-22 and TNF-a represent a key cytokine combination for epidermal integrity during infection with Candida albicans. / Eyerich, Stefanie (Corresponding Author); Wagener, Jeanette; Wenzel, Vera; Scarponi, Claudia; Pennino, Davide; Albanesi, Cristina; Schaller, Martin; Behrendt, Heidrun; Ring, Johannes; Schmidt-Weber, Carsten B; Cavani, Andrea; Mempel, Martin; Traidl-Hoffmann, Claudia; Eyerich, Kilian.

In: European Journal of Immunology, Vol. 41, No. 7, 07.2011, p. 1894-1901.

Research output: Contribution to journalArticle

Eyerich, S, Wagener, J, Wenzel, V, Scarponi, C, Pennino, D, Albanesi, C, Schaller, M, Behrendt, H, Ring, J, Schmidt-Weber, CB, Cavani, A, Mempel, M, Traidl-Hoffmann, C & Eyerich, K 2011, 'IL-22 and TNF-a represent a key cytokine combination for epidermal integrity during infection with Candida albicans', European Journal of Immunology, vol. 41, no. 7, pp. 1894-1901. https://doi.org/10.1002/eji.201041197
Eyerich, Stefanie ; Wagener, Jeanette ; Wenzel, Vera ; Scarponi, Claudia ; Pennino, Davide ; Albanesi, Cristina ; Schaller, Martin ; Behrendt, Heidrun ; Ring, Johannes ; Schmidt-Weber, Carsten B ; Cavani, Andrea ; Mempel, Martin ; Traidl-Hoffmann, Claudia ; Eyerich, Kilian. / IL-22 and TNF-a represent a key cytokine combination for epidermal integrity during infection with Candida albicans. In: European Journal of Immunology. 2011 ; Vol. 41, No. 7. pp. 1894-1901.
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T1 - IL-22 and TNF-a represent a key cytokine combination for epidermal integrity during infection with Candida albicans

AU - Eyerich, Stefanie

AU - Wagener, Jeanette

AU - Wenzel, Vera

AU - Scarponi, Claudia

AU - Pennino, Davide

AU - Albanesi, Cristina

AU - Schaller, Martin

AU - Behrendt, Heidrun

AU - Ring, Johannes

AU - Schmidt-Weber, Carsten B

AU - Cavani, Andrea

AU - Mempel, Martin

AU - Traidl-Hoffmann, Claudia

AU - Eyerich, Kilian

N1 - Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2011/7

Y1 - 2011/7

N2 - T cells exercise their full impact on target cells through a combination of secreted cytokines. The recently described T helper cell subset Th22 is characterized by a combinatorial secretion of IL-22 and TNF-a. Here, we demonstrate that IL-22 increases the TNF-a-dependent induction and secretion of several immune-modulatory molecules such as initial complement factors C1r and C1s, antimicrobial peptides S100A7 and HBD-2 (human ß defensin 2), and antimicrobial chemokines CXCL-9/-10/-11 in primary human keratinocytes. The synergism of IL-22 and TNF-a is transmitted intracellularly by MAP kinases and downstream by transcription factors of the AP-1 family. The induction of innate immunity is relevant in an in vitro infection model, where keratinocytes stimulated with Th22 supernatants or recombinant IL-22 plus TNF-a effectively inhibit the growth of Candida albicans and maintain survival of epithelia. Accordingly, the combinatorial stimulation of keratinocytes with IL-22 and TNF-a most efficiently conserves the integrity of the epidermal barrier in a three-dimensional skin infection model as compared with IFN-¿, IL-17, IL-22 or TNF-a alone. In summary, we demonstrate that IL-22 and TNF-a represent a potent, synergistic cytokine combination for cutaneous immunity.

AB - T cells exercise their full impact on target cells through a combination of secreted cytokines. The recently described T helper cell subset Th22 is characterized by a combinatorial secretion of IL-22 and TNF-a. Here, we demonstrate that IL-22 increases the TNF-a-dependent induction and secretion of several immune-modulatory molecules such as initial complement factors C1r and C1s, antimicrobial peptides S100A7 and HBD-2 (human ß defensin 2), and antimicrobial chemokines CXCL-9/-10/-11 in primary human keratinocytes. The synergism of IL-22 and TNF-a is transmitted intracellularly by MAP kinases and downstream by transcription factors of the AP-1 family. The induction of innate immunity is relevant in an in vitro infection model, where keratinocytes stimulated with Th22 supernatants or recombinant IL-22 plus TNF-a effectively inhibit the growth of Candida albicans and maintain survival of epithelia. Accordingly, the combinatorial stimulation of keratinocytes with IL-22 and TNF-a most efficiently conserves the integrity of the epidermal barrier in a three-dimensional skin infection model as compared with IFN-¿, IL-17, IL-22 or TNF-a alone. In summary, we demonstrate that IL-22 and TNF-a represent a potent, synergistic cytokine combination for cutaneous immunity.

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KW - Candidiasis, Cutaneous

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KW - Enzyme-Linked Immunosorbent Assay

KW - Epidermis

KW - Humans

KW - Immunity, Innate

KW - Immunoblotting

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KW - Keratinocytes

KW - Male

KW - Mitogen-Activated Protein Kinases

KW - Polymerase Chain Reaction

KW - S100 Proteins

KW - T-Lymphocytes, Helper-Inducer

KW - Transcription Factor AP-1

KW - Tumor Necrosis Factor-alpha

KW - beta-Defensins

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DO - 10.1002/eji.201041197

M3 - Article

VL - 41

SP - 1894

EP - 1901

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 7

ER -