Immediate visualization of recombination events and chromosome segregation defects in fission yeast meiosis

Dmitriy Li, Marianne Roca, Raif Yuecel, Alexander Lorenz* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)
5 Downloads (Pure)

Abstract

Schizosaccharomyces pombe, also known as fission yeast, is an established model for studying chromosome biological processes. Over the years, research employing fission yeast has made important contributions to our knowledge about chromosome segregation during meiosis, as well as meiotic recombination and its regulation. Quantification of meiotic recombination frequency is not a straightforward undertaking, either requiring viable progeny for a genetic plating assay, or relying on laborious Southern blot analysis of recombination intermediates. Neither of these methods lends itself to high-throughput screens to identify novel meiotic factors. Here, we establish visual assays novel to Sz. pombe for characterizing chromosome segregation and meiotic recombination phenotypes. Genes expressing red, yellow, and/or cyan fluorophores from spore-autonomous promoters have been integrated into the fission yeast genomes, either close to the centromere of chromosome 1 to monitor chromosome segregation, or on the arm of chromosome 3 to form a genetic interval at which recombination frequency can be determined. The visual recombination assay allows straightforward and immediate assessment of the genetic outcome of a single meiosis by epi-fluorescence microscopy without requiring tetrad dissection. We also demonstrate that the recombination frequency analysis can be automatized by utilizing imaging flow cytometry to enable high-throughput screens. These assays have several advantages over traditional methods for analyzing meiotic phenotypes.
Original languageEnglish
Pages (from-to)385-396
Number of pages8
JournalChromosoma
Volume128
Issue number3
Early online date9 Feb 2019
DOIs
Publication statusPublished - Sep 2019

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Chromosome Segregation
Schizosaccharomyces
Meiosis
Genetic Recombination
Phenotype
Biological Phenomena
Chromosomes, Human, Pair 3
Centromere
Chromosomes, Human, Pair 1
Southern Blotting
Spores
Fluorescence Microscopy
Dissection
Flow Cytometry
Chromosomes
Outcome Assessment (Health Care)
Genome
Research
Genes

Keywords

  • Schizosaccharomyces pombe
  • chromosome segregation
  • meiotic recombination
  • spore autonomous promoters
  • imaging flow cytometry
  • Meiotic recombination
  • Spore-autonomous promoters
  • Imaging flow cytometry
  • Chromosome segregation

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Immediate visualization of recombination events and chromosome segregation defects in fission yeast meiosis. / Li, Dmitriy; Roca, Marianne ; Yuecel, Raif; Lorenz, Alexander (Corresponding Author).

In: Chromosoma, Vol. 128, No. 3, 09.2019, p. 385-396.

Research output: Contribution to journalArticle

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abstract = "Schizosaccharomyces pombe, also known as fission yeast, is an established model for studying chromosome biological processes. Over the years, research employing fission yeast has made important contributions to our knowledge about chromosome segregation during meiosis, as well as meiotic recombination and its regulation. Quantification of meiotic recombination frequency is not a straightforward undertaking, either requiring viable progeny for a genetic plating assay, or relying on laborious Southern blot analysis of recombination intermediates. Neither of these methods lends itself to high-throughput screens to identify novel meiotic factors. Here, we establish visual assays novel to Sz. pombe for characterizing chromosome segregation and meiotic recombination phenotypes. Genes expressing red, yellow, and/or cyan fluorophores from spore-autonomous promoters have been integrated into the fission yeast genomes, either close to the centromere of chromosome 1 to monitor chromosome segregation, or on the arm of chromosome 3 to form a genetic interval at which recombination frequency can be determined. The visual recombination assay allows straightforward and immediate assessment of the genetic outcome of a single meiosis by epi-fluorescence microscopy without requiring tetrad dissection. We also demonstrate that the recombination frequency analysis can be automatized by utilizing imaging flow cytometry to enable high-throughput screens. These assays have several advantages over traditional methods for analyzing meiotic phenotypes.",
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note = "We are grateful to Scott Keeney, Franz Klein, J{\"u}rg Kohli, Josef Loidl, Kim Nasmyth, Ken E. Sawin, Gerald R. Smith, Takashi Toda, Matthew C. Whitby, and the National BioResource Project (NBRP) Japan for providing strains and/or plasmids; to M.N. Asogwa, A. Bebes, and L. Duncan for technical assistance; and to M. De Carvalho for spotting a critical typographical error in an earlier version of the manuscript. Microscopy was performed at the University of Aberdeen Microscopy & Histology facility (Kevin Mackenzie). This work was supported by a Carnegie Trust for the Universities of Scotland Research Incentive Grant (No. 70021), and the University of Aberdeen (College of Life Sciences and Medicine Start-up grant). Open Access via the Springer Compact Agreement.",
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