Immune Gene Expression in Trout Cell Lines Infected with the Fish Pathogenic Oomycete Saprolegnia Parasitica

Irene de Bruijn, Rodrigo Belmonte, Vicky L. Anderson, Marcia Saraiva, Tiehui Wang, Pieter van West, Christopher J Secombes

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The oomycete Saprolegnia parasitica causes significant losses in the aquaculture industry, mainly affecting salmon, trout and catfish. Since the ban of malachite green, effective control measures are currently not available prompting a re-evaluation of the potential for immunological intervention. In this study, the immune response of salmonid cells is investigated at the transcript level, by analysis of a large set of immune response genes in four different rainbow trout cell lines (RTG-2, RTGill, RTL and RTS11) upon infection with S. parasitica. Proinflammatory cytokine transcripts were induced in all four cell lines, including IL-1ß1, IL-8, IL-11, TNF-a2, as well as other components of the innate defences, including COX-2, the acute phase protein serum amyloid A and C-type lectin CD209a and CD209b. However, differences between the four cell lines were found. For example, the fold change of induction was much higher in the epithelial RTL and macrophage-like RTS11 cell lines compared to the fibroblast cell lines RTG-2 and RTGill. Several antimicrobial peptides (AMPs) were also up-regulated in response to Saprolegnia infection, including hepcidin and cathelicidin 1 (rtCATH1) and 2 (rtCATH2). An rtCATH2 peptide was synthesised and tested for activity and whilst it showed no killing activity for zoospores, it was able to delay sporulation of S. parasitica. These results demonstrate that particular immune genes are up-regulated in response to S. parasitica infection and that AMPs may play a crucial role in the first line of defence against oomycetes in fish.
Original languageEnglish
Pages (from-to)44-54
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume38
Issue number1
Early online date18 Apr 2012
DOIs
Publication statusPublished - Sep 2012

Fingerprint

Saprolegnia
Oomycetes
Trout
Gene Expression
Cell Line
Interleukin-11
Peptides
Infection
Hepcidins
C-Type Lectins
Serum Amyloid A Protein
Catfishes
Aquaculture
Acute-Phase Proteins
Salmon
Oncorhynchus mykiss
Interleukin-8
Genes
Industry
Fishes

Keywords

  • oomycete
  • Saprolegnia parasitica
  • cathelicidin
  • trout
  • salmon

Cite this

Immune Gene Expression in Trout Cell Lines Infected with the Fish Pathogenic Oomycete Saprolegnia Parasitica. / Bruijn, Irene de; Belmonte, Rodrigo; Anderson, Vicky L.; Saraiva, Marcia; Wang, Tiehui; West, Pieter van; Secombes, Christopher J.

In: Developmental and Comparative Immunology, Vol. 38, No. 1, 09.2012, p. 44-54.

Research output: Contribution to journalArticle

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abstract = "The oomycete Saprolegnia parasitica causes significant losses in the aquaculture industry, mainly affecting salmon, trout and catfish. Since the ban of malachite green, effective control measures are currently not available prompting a re-evaluation of the potential for immunological intervention. In this study, the immune response of salmonid cells is investigated at the transcript level, by analysis of a large set of immune response genes in four different rainbow trout cell lines (RTG-2, RTGill, RTL and RTS11) upon infection with S. parasitica. Proinflammatory cytokine transcripts were induced in all four cell lines, including IL-1{\ss}1, IL-8, IL-11, TNF-a2, as well as other components of the innate defences, including COX-2, the acute phase protein serum amyloid A and C-type lectin CD209a and CD209b. However, differences between the four cell lines were found. For example, the fold change of induction was much higher in the epithelial RTL and macrophage-like RTS11 cell lines compared to the fibroblast cell lines RTG-2 and RTGill. Several antimicrobial peptides (AMPs) were also up-regulated in response to Saprolegnia infection, including hepcidin and cathelicidin 1 (rtCATH1) and 2 (rtCATH2). An rtCATH2 peptide was synthesised and tested for activity and whilst it showed no killing activity for zoospores, it was able to delay sporulation of S. parasitica. These results demonstrate that particular immune genes are up-regulated in response to S. parasitica infection and that AMPs may play a crucial role in the first line of defence against oomycetes in fish.",
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AU - Saraiva, Marcia

AU - Wang, Tiehui

AU - West, Pieter van

AU - Secombes, Christopher J

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