Abstract
Immunization using genetic expression libraries may be an improvement over conventional DNA immunization using a single gene because more epitopes are simultaneously presented to the immune system. In this study, we evaluated the effectiveness of an HIV-2 vaccine made from a genomic expression library in baboons. We found that HIV-2 expression library immunization induced HIV-2-specific memory responses but low levels of CD8+ cell anti-viral responses and neutralizing antibodies. After intravenous virus challenge using a homologous pathogenic variant, HIV-2UC2/9429, viral loads were similar in the HIV-2-immunized and control baboons. We conclude that although immunization using HIV-2 expression libraries induces immune responses, this approach does not provide protection in baboons against intravenous challenge with HIV-2.
Original language | English |
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Pages (from-to) | 323-329 |
Number of pages | 7 |
Journal | Journal of Medical Primatology |
Volume | 31 |
Issue number | 6 |
DOIs | |
Publication status | Published - Dec 2002 |
Bibliographical note
AcknowledgmentsThe authors thank Drs. Michelle Leland, Karen Rice, Kenneth D. Carey and the staff at the Southwest Foundation for Biomedical Research for providing veterinary care; Dr. Tran Chanh and Sylvia Trevino for the evaluation of cytotoxic T-cell activity; Drs. Christine Stahl-Henning and Gerhardt Hunsmann of the German Primate Center for providing the recombinant HIV-2BEN vaccinia constructs; Dr. Margery Chaikin of SmithKline Beecham for providing the recombinant HIV-2ST gp120 polypeptide; Dr. Jay Levy and M. S. Mimizeiget of UCSF and Dr. Susan Barnett of the Chiron Corporation for helpful discussions.
Keywords
- AIDS
- DNA vaccine
- Expression library immunization
- Gene immunization
- Papio