Immunohistochemical evidence for an association of ribosomes with microfilaments in 3T3 fibroblasts

J E  HESKETH; Z  HORNE; Gillian Patricia Campbell

doi:10.1016/0309-1651(91)90105-R

Immunohistochemical evidence for an association of ribosomes with microfilaments in 3T3 fibroblasts

J E HESKETH, Z HORNE, Gillian Patricia Campbell

The Rowett Institute of Nutrition and Health

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26 Citations (Scopus)

Abstract

Ribosome distribution in cultured fibroblasts was investigated immunohistochemically using antibodies which recognize the 60S ribosomal subunit. After treatment of cells with buffer containing 25mM KCl and 0.05% Nonidet-P40 immunostained material was present in punctate patterns and linear arrays consistent with some ribosomes being associated with the cytoskeleton. Treatment of the cells with 130mM KCl caused loss of both the beaded lines of immunostaining and micro-filaments. Double immunostaining showed ribosomes to be closely associated with microfilaments.

Original language	English
Pages (from-to)	141-150
Number of pages	10
Journal	Cell Biology International Reports
Volume	15
Issue number	2
DOIs	https://doi.org/10.1016/0309-1651(91)90105-R
Publication status	Published - Feb 1991

Keywords

proteins
polyribosomes
cytoskeleton
cells
polysomes

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10.1016/0309-1651(91)90105-R

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title = "Immunohistochemical evidence for an association of ribosomes with microfilaments in 3T3 fibroblasts",

abstract = "Ribosome distribution in cultured fibroblasts was investigated immunohistochemically using antibodies which recognize the 60S ribosomal subunit. After treatment of cells with buffer containing 25mM KCl and 0.05% Nonidet-P40 immunostained material was present in punctate patterns and linear arrays consistent with some ribosomes being associated with the cytoskeleton. Treatment of the cells with 130mM KCl caused loss of both the beaded lines of immunostaining and micro-filaments. Double immunostaining showed ribosomes to be closely associated with microfilaments. ",

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AU - HESKETH, J E

AU - HORNE, Z

AU - Campbell, Gillian Patricia

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Y1 - 1991/2

N2 - Ribosome distribution in cultured fibroblasts was investigated immunohistochemically using antibodies which recognize the 60S ribosomal subunit. After treatment of cells with buffer containing 25mM KCl and 0.05% Nonidet-P40 immunostained material was present in punctate patterns and linear arrays consistent with some ribosomes being associated with the cytoskeleton. Treatment of the cells with 130mM KCl caused loss of both the beaded lines of immunostaining and micro-filaments. Double immunostaining showed ribosomes to be closely associated with microfilaments.

AB - Ribosome distribution in cultured fibroblasts was investigated immunohistochemically using antibodies which recognize the 60S ribosomal subunit. After treatment of cells with buffer containing 25mM KCl and 0.05% Nonidet-P40 immunostained material was present in punctate patterns and linear arrays consistent with some ribosomes being associated with the cytoskeleton. Treatment of the cells with 130mM KCl caused loss of both the beaded lines of immunostaining and micro-filaments. Double immunostaining showed ribosomes to be closely associated with microfilaments.

KW - proteins

KW - polyribosomes

KW - cytoskeleton

KW - cells

KW - polysomes

U2 - 10.1016/0309-1651(91)90105-R

DO - 10.1016/0309-1651(91)90105-R

M3 - Article

SN - 0309-1651

VL - 15

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EP - 150

JO - Cell Biology International Reports

JF - Cell Biology International Reports

IS - 2

ER -

Immunohistochemical evidence for an association of ribosomes with microfilaments in 3T3 fibroblasts

Abstract

Keywords

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