Immunohistochemical evidence for an association of ribosomes with microfilaments in 3T3 fibroblasts

J E HESKETH, Z HORNE, Gillian Patricia Campbell

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    Ribosome distribution in cultured fibroblasts was investigated immunohistochemically using antibodies which recognize the 60S ribosomal subunit. After treatment of cells with buffer containing 25mM KCl and 0.05% Nonidet-P40 immunostained material was present in punctate patterns and linear arrays consistent with some ribosomes being associated with the cytoskeleton. Treatment of the cells with 130mM KCl caused loss of both the beaded lines of immunostaining and micro-filaments. Double immunostaining showed ribosomes to be closely associated with microfilaments.

    Original languageEnglish
    Pages (from-to)141-150
    Number of pages10
    JournalCell Biology International Reports
    Volume15
    Issue number2
    DOIs
    Publication statusPublished - Feb 1991

    Keywords

    • proteins
    • polyribosomes
    • cytoskeleton
    • cells
    • polysomes

    Cite this

    Immunohistochemical evidence for an association of ribosomes with microfilaments in 3T3 fibroblasts. / HESKETH, J E ; HORNE, Z ; Campbell, Gillian Patricia.

    In: Cell Biology International Reports, Vol. 15, No. 2, 02.1991, p. 141-150.

    Research output: Contribution to journalArticle

    HESKETH, J E ; HORNE, Z ; Campbell, Gillian Patricia. / Immunohistochemical evidence for an association of ribosomes with microfilaments in 3T3 fibroblasts. In: Cell Biology International Reports. 1991 ; Vol. 15, No. 2. pp. 141-150.
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