Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination

Kimberly A. Veenstra, Ayham Alnabulsi, Lincoln Tubbs, Juliette Ben Arous, Christopher J. Secombes (Corresponding Author)

Research output: Contribution to journalArticle

Abstract

Mammalian perivisceral adipose has been shown to play an important role in the regulation of the peritoneal immune responses. Recently it has been demonstrated that peritoneal antigens are collected by leukocytes within the visceral adipose mass, and a broad range of immunomodulatory genes are differentially expressed in adipose tissue after intraperitoneal vaccination in rainbow trout. To assess the immune cell component in adipose, immunohistochemical analysis was used to examine B-cell, T-cell and antigen presenting cell (APC) numbers and distribution in rainbow trout adipose tissue 24 and 72 h post vaccination in comparison to control fish. The results of this study support previous work on mammals with omental milky spots in naïve fish found to contain APCs and T-cells which then increased in size, number and complexity following vaccination. It suggests that following peritoneal stimulation the visceral adipose mass in fish likely plays an important role in vaccine antigen uptake and presentation by APCs, as well as subsequent T-cell activation and differentiation.
Original languageEnglish
Pages (from-to)559-564
Number of pages6
JournalFish & Shellfish Immunology
Volume87
Early online date4 Feb 2019
DOIs
Publication statusPublished - Apr 2019

Fingerprint

T-cells
vaccination
antigen
Fish
adipose tissue
rainbow
Oncorhynchus mykiss
T-lymphocytes
Tissue
fish
antigens
Mammals
antigen-presenting cells
vaccine
cells
HLA Antigens
immune response
B-lymphocytes
leukocytes
mammal

Keywords

  • rainbow trout
  • adipose tissue
  • immunohistochemistry
  • cell markers
  • vaccination
  • milky spots
  • APC
  • T-cell
  • B-cell

Cite this

Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination. / Veenstra, Kimberly A.; Alnabulsi, Ayham; Tubbs, Lincoln; Ben Arous, Juliette; Secombes, Christopher J. (Corresponding Author).

In: Fish & Shellfish Immunology, Vol. 87, 04.2019, p. 559-564.

Research output: Contribution to journalArticle

Veenstra, Kimberly A. ; Alnabulsi, Ayham ; Tubbs, Lincoln ; Ben Arous, Juliette ; Secombes, Christopher J. / Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination. In: Fish & Shellfish Immunology. 2019 ; Vol. 87. pp. 559-564.
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abstract = "Mammalian perivisceral adipose has been shown to play an important role in the regulation of the peritoneal immune responses. Recently it has been demonstrated that peritoneal antigens are collected by leukocytes within the visceral adipose mass, and a broad range of immunomodulatory genes are differentially expressed in adipose tissue after intraperitoneal vaccination in rainbow trout. To assess the immune cell component in adipose, immunohistochemical analysis was used to examine B-cell, T-cell and antigen presenting cell (APC) numbers and distribution in rainbow trout adipose tissue 24 and 72 h post vaccination in comparison to control fish. The results of this study support previous work on mammals with omental milky spots in na{\"i}ve fish found to contain APCs and T-cells which then increased in size, number and complexity following vaccination. It suggests that following peritoneal stimulation the visceral adipose mass in fish likely plays an important role in vaccine antigen uptake and presentation by APCs, as well as subsequent T-cell activation and differentiation.",
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