Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination

Kimberly A. Veenstra; Ayham Alnabulsi; Lincoln Tubbs; Juliette Ben Arous; Christopher J. Secombes

doi:10.1016/j.fsi.2019.02.001

Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination

Kimberly A. Veenstra, Ayham Alnabulsi, Lincoln Tubbs, Juliette Ben Arous, Christopher J. Secombes (Corresponding Author)

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

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Abstract

Mammalian perivisceral adipose has been shown to play an important role in the regulation of the peritoneal immune responses. Recently it has been demonstrated that peritoneal antigens are collected by leukocytes within the visceral adipose mass, and a broad range of immunomodulatory genes are differentially expressed in adipose tissue after intraperitoneal vaccination in rainbow trout. To assess the immune cell component in adipose, immunohistochemical analysis was used to examine B-cell, T-cell and antigen presenting cell (APC) numbers and distribution in rainbow trout adipose tissue 24 and 72 h post vaccination in comparison to control fish. The results of this study support previous work on mammals with omental milky spots in naïve fish found to contain APCs and T-cells which then increased in size, number and complexity following vaccination. It suggests that following peritoneal stimulation the visceral adipose mass in fish likely plays an important role in vaccine antigen uptake and presentation by APCs, as well as subsequent T-cell activation and differentiation.

Original language	English
Pages (from-to)	559-564
Number of pages	6
Journal	Fish & Shellfish Immunology
Volume	87
Early online date	4 Feb 2019
DOIs	https://doi.org/10.1016/j.fsi.2019.02.001
Publication status	Published - Apr 2019

Keywords

rainbow trout
adipose tissue
immunohistochemistry
cell markers
vaccination
milky spots
APC
T-cell
B-cell

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Accepted author manuscript, 742 KBLicence: CC BY-NC-ND

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@article{f417fe73490e4a56afb88a44e8dc2499,

title = "Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination",

abstract = "Mammalian perivisceral adipose has been shown to play an important role in the regulation of the peritoneal immune responses. Recently it has been demonstrated that peritoneal antigens are collected by leukocytes within the visceral adipose mass, and a broad range of immunomodulatory genes are differentially expressed in adipose tissue after intraperitoneal vaccination in rainbow trout. To assess the immune cell component in adipose, immunohistochemical analysis was used to examine B-cell, T-cell and antigen presenting cell (APC) numbers and distribution in rainbow trout adipose tissue 24 and 72 h post vaccination in comparison to control fish. The results of this study support previous work on mammals with omental milky spots in na{\"i}ve fish found to contain APCs and T-cells which then increased in size, number and complexity following vaccination. It suggests that following peritoneal stimulation the visceral adipose mass in fish likely plays an important role in vaccine antigen uptake and presentation by APCs, as well as subsequent T-cell activation and differentiation.",

keywords = "rainbow trout, adipose tissue, immunohistochemistry, cell markers, vaccination, milky spots, APC, T-cell, B-cell",

author = "Veenstra, {Kimberly A.} and Ayham Alnabulsi and Lincoln Tubbs and {Ben Arous}, Juliette and Secombes, {Christopher J.}",

note = "KAV was supported by ELANCO and SEPPIC to undertake a PhD program at the University of Aberdeen. KAV would like to thank Dr. Abdo Alnabulsi and Dr. Jason Holland for their assistance with immunohistochemistry.",

year = "2019",

month = apr,

doi = "10.1016/j.fsi.2019.02.001",

language = "English",

volume = "87",

pages = "559--564",

journal = "Fish & Shellfish Immunology",

issn = "1050-4648",

publisher = "ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD",

}

TY - JOUR

T1 - Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination

AU - Veenstra, Kimberly A.

AU - Alnabulsi, Ayham

AU - Tubbs, Lincoln

AU - Ben Arous, Juliette

AU - Secombes, Christopher J.

N1 - KAV was supported by ELANCO and SEPPIC to undertake a PhD program at the University of Aberdeen. KAV would like to thank Dr. Abdo Alnabulsi and Dr. Jason Holland for their assistance with immunohistochemistry.

PY - 2019/4

Y1 - 2019/4

N2 - Mammalian perivisceral adipose has been shown to play an important role in the regulation of the peritoneal immune responses. Recently it has been demonstrated that peritoneal antigens are collected by leukocytes within the visceral adipose mass, and a broad range of immunomodulatory genes are differentially expressed in adipose tissue after intraperitoneal vaccination in rainbow trout. To assess the immune cell component in adipose, immunohistochemical analysis was used to examine B-cell, T-cell and antigen presenting cell (APC) numbers and distribution in rainbow trout adipose tissue 24 and 72 h post vaccination in comparison to control fish. The results of this study support previous work on mammals with omental milky spots in naïve fish found to contain APCs and T-cells which then increased in size, number and complexity following vaccination. It suggests that following peritoneal stimulation the visceral adipose mass in fish likely plays an important role in vaccine antigen uptake and presentation by APCs, as well as subsequent T-cell activation and differentiation.

AB - Mammalian perivisceral adipose has been shown to play an important role in the regulation of the peritoneal immune responses. Recently it has been demonstrated that peritoneal antigens are collected by leukocytes within the visceral adipose mass, and a broad range of immunomodulatory genes are differentially expressed in adipose tissue after intraperitoneal vaccination in rainbow trout. To assess the immune cell component in adipose, immunohistochemical analysis was used to examine B-cell, T-cell and antigen presenting cell (APC) numbers and distribution in rainbow trout adipose tissue 24 and 72 h post vaccination in comparison to control fish. The results of this study support previous work on mammals with omental milky spots in naïve fish found to contain APCs and T-cells which then increased in size, number and complexity following vaccination. It suggests that following peritoneal stimulation the visceral adipose mass in fish likely plays an important role in vaccine antigen uptake and presentation by APCs, as well as subsequent T-cell activation and differentiation.

KW - rainbow trout

KW - adipose tissue

KW - immunohistochemistry

KW - cell markers

KW - vaccination

KW - milky spots

KW - APC

KW - T-cell

KW - B-cell

U2 - 10.1016/j.fsi.2019.02.001

DO - 10.1016/j.fsi.2019.02.001

M3 - Article

VL - 87

SP - 559

EP - 564

JO - Fish & Shellfish Immunology

JF - Fish & Shellfish Immunology

SN - 1050-4648

ER -

Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination

Abstract

Keywords

UN SDGs

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