Immunohistochemical localization of cytochrome P450CYP1B1 in breast cancer with monoclonal antibodies specific for CYP1B1

M C E McFadyen, S Breeman, S Payne, C Stirk, I D Miller, W T Melvin, G I Murray

Research output: Contribution to journalArticle

Abstract

Cytochrome P450 CYP1B1 is a recently identified member of the CYP1 P450 family. We have shown that this P450 displays increased expression in several types of human cancer, indicating that CYP1B1 is a potential tumor biomarker. In this study we developed monoclonal antibodies (MAbs) to CYP1B1 that are effective on formalin-fixed, paraffin-embedded tissue sections and investigated the presence of CYP1B1 in a series of primary breast cancers. The MAbs were generated using a synthetic peptide coupled to carrier protein as the immunogen. The MAbs specifically recognized CYP1B1 and did not recognize either CYP1A1 or CYP1A2, related CYP1 forms. The MAbs were tested by immunohistochemistry and were found to be effective on formalin-fixed, paraffin-embedded tissue sections. The majority of breast cancers showed positive immunoreactivity for CYP1B1, and in each case CYP1B1 was specifically localized to tumor cells. The presence of CYP1B1 in breast cancer cells is likely to contribute to their metabolism of estradiol because CYP1B1 is a specific estradiol hydroxylase.

Original languageEnglish
Pages (from-to)1457-1464
Number of pages8
JournalJournal of Histochemistry and Cytochemistry
Volume47
Publication statusPublished - 1999

Keywords

  • breast cancer
  • CYP1B1
  • cytochrome P450
  • immunohistochemistry
  • ADRENAL CYTOCHROME-P450
  • RAT CYP1B1
  • EXPRESSION
  • SEQUENCE
  • CDNA
  • GENE
  • 1B1
  • HYDROCARBONS
  • METABOLISM
  • SUBFAMILY

Cite this

Immunohistochemical localization of cytochrome P450CYP1B1 in breast cancer with monoclonal antibodies specific for CYP1B1. / McFadyen, M C E ; Breeman, S ; Payne, S ; Stirk, C ; Miller, I D ; Melvin, W T ; Murray, G I .

In: Journal of Histochemistry and Cytochemistry, Vol. 47, 1999, p. 1457-1464.

Research output: Contribution to journalArticle

McFadyen, M C E ; Breeman, S ; Payne, S ; Stirk, C ; Miller, I D ; Melvin, W T ; Murray, G I . / Immunohistochemical localization of cytochrome P450CYP1B1 in breast cancer with monoclonal antibodies specific for CYP1B1. In: Journal of Histochemistry and Cytochemistry. 1999 ; Vol. 47. pp. 1457-1464.
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abstract = "Cytochrome P450 CYP1B1 is a recently identified member of the CYP1 P450 family. We have shown that this P450 displays increased expression in several types of human cancer, indicating that CYP1B1 is a potential tumor biomarker. In this study we developed monoclonal antibodies (MAbs) to CYP1B1 that are effective on formalin-fixed, paraffin-embedded tissue sections and investigated the presence of CYP1B1 in a series of primary breast cancers. The MAbs were generated using a synthetic peptide coupled to carrier protein as the immunogen. The MAbs specifically recognized CYP1B1 and did not recognize either CYP1A1 or CYP1A2, related CYP1 forms. The MAbs were tested by immunohistochemistry and were found to be effective on formalin-fixed, paraffin-embedded tissue sections. The majority of breast cancers showed positive immunoreactivity for CYP1B1, and in each case CYP1B1 was specifically localized to tumor cells. The presence of CYP1B1 in breast cancer cells is likely to contribute to their metabolism of estradiol because CYP1B1 is a specific estradiol hydroxylase.",
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T1 - Immunohistochemical localization of cytochrome P450CYP1B1 in breast cancer with monoclonal antibodies specific for CYP1B1

AU - McFadyen, M C E

AU - Breeman, S

AU - Payne, S

AU - Stirk, C

AU - Miller, I D

AU - Melvin, W T

AU - Murray, G I

PY - 1999

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N2 - Cytochrome P450 CYP1B1 is a recently identified member of the CYP1 P450 family. We have shown that this P450 displays increased expression in several types of human cancer, indicating that CYP1B1 is a potential tumor biomarker. In this study we developed monoclonal antibodies (MAbs) to CYP1B1 that are effective on formalin-fixed, paraffin-embedded tissue sections and investigated the presence of CYP1B1 in a series of primary breast cancers. The MAbs were generated using a synthetic peptide coupled to carrier protein as the immunogen. The MAbs specifically recognized CYP1B1 and did not recognize either CYP1A1 or CYP1A2, related CYP1 forms. The MAbs were tested by immunohistochemistry and were found to be effective on formalin-fixed, paraffin-embedded tissue sections. The majority of breast cancers showed positive immunoreactivity for CYP1B1, and in each case CYP1B1 was specifically localized to tumor cells. The presence of CYP1B1 in breast cancer cells is likely to contribute to their metabolism of estradiol because CYP1B1 is a specific estradiol hydroxylase.

AB - Cytochrome P450 CYP1B1 is a recently identified member of the CYP1 P450 family. We have shown that this P450 displays increased expression in several types of human cancer, indicating that CYP1B1 is a potential tumor biomarker. In this study we developed monoclonal antibodies (MAbs) to CYP1B1 that are effective on formalin-fixed, paraffin-embedded tissue sections and investigated the presence of CYP1B1 in a series of primary breast cancers. The MAbs were generated using a synthetic peptide coupled to carrier protein as the immunogen. The MAbs specifically recognized CYP1B1 and did not recognize either CYP1A1 or CYP1A2, related CYP1 forms. The MAbs were tested by immunohistochemistry and were found to be effective on formalin-fixed, paraffin-embedded tissue sections. The majority of breast cancers showed positive immunoreactivity for CYP1B1, and in each case CYP1B1 was specifically localized to tumor cells. The presence of CYP1B1 in breast cancer cells is likely to contribute to their metabolism of estradiol because CYP1B1 is a specific estradiol hydroxylase.

KW - breast cancer

KW - CYP1B1

KW - cytochrome P450

KW - immunohistochemistry

KW - ADRENAL CYTOCHROME-P450

KW - RAT CYP1B1

KW - EXPRESSION

KW - SEQUENCE

KW - CDNA

KW - GENE

KW - 1B1

KW - HYDROCARBONS

KW - METABOLISM

KW - SUBFAMILY

M3 - Article

VL - 47

SP - 1457

EP - 1464

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

ER -