Immunolocalization of immune cells and cell cycle proteins in the bulbus arteriosus of Atlantic salmon (Salmo salar L.)

Muhammad Naveed Yousaf, Erling Olaf Koppang, Jun Zou, Christopher John Secombes, Mark D. Powell

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The bulbus arteriosus is the most anterior chamber of the teleost heart. The present study aimed to establish the presence, and to provide semi-quantitative information on the abundance, of several immune and cell-cycle proteins in the bulbus arteriosus of healthy Atlantic salmon (Salmo salar L.). Using immunohistochemistry, lymphocyte-like cells were identified in the bulbus arteriosus using antibodies to CD3ε and MHC class IIβ. Few PCNA positive cells were identified in post-smolt fish as compared to moderate levels of staining in fresh water fry. Interestingly no staining was evident in adult fish (1-3 kg), thus there was a loss of cells expressing cell-cycle regulatory proteins with ontogeny/progressive life-history stages. Eosinophilic granulocytes (EGCs) were identified in the bulbus arteriosus using TNFα and HIF1α antibodies. Anti-caspase 3 immune-reaction identified a strong endothelial cytoplasmic staining in the bulbus arteriosus. Taken together, the immunolocalization of immune-related molecules (CD3, MHC class II and TNFα), cell-cycle regulatory proteins (PCNA and HIF1α) and apoptosis markers (TUNEL, caspase 3) suggest that the bulbus arteriosus may have an immune component within its functional repertoire.

Original languageEnglish
Pages (from-to)64-69
Number of pages6
JournalFish & Shellfish Immunology
Volume51
Early online date10 Feb 2016
DOIs
Publication statusPublished - Apr 2016

Fingerprint

Cell Cycle Proteins
Salmo salar
cell cycle
regulatory proteins
Proliferating Cell Nuclear Antigen
caspase-3
Caspase 3
Fish
antibody
protein
Cells
Antigen-antibody reactions
antibodies
Lymphocytes
proteins
Antibodies
smolt
apoptosis
smolts
cells

Keywords

  • Atlantic salmon
  • Bulbus arteriosus
  • Caspase
  • CD3
  • HIF1α
  • Immunohistochemistry
  • MHC II
  • TNFα

ASJC Scopus subject areas

  • Aquatic Science
  • Environmental Chemistry

Cite this

Immunolocalization of immune cells and cell cycle proteins in the bulbus arteriosus of Atlantic salmon (Salmo salar L.). / Yousaf, Muhammad Naveed; Koppang, Erling Olaf; Zou, Jun; Secombes, Christopher John; Powell, Mark D.

In: Fish & Shellfish Immunology, Vol. 51, 04.2016, p. 64-69.

Research output: Contribution to journalArticle

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