Immunological Determination of the Pharmaceutical Diclofenac in Environmental and Biological Samples

Dietmar Knopp, Anping Deng, Marion Letzel, Mark Taggart, Markus Himmelsbach, Qing-Zhi Zhu, Iris Peröbner, Blazej Kudlak, Siegfried Frey, Manfred Sengl, Wolfgang Buchberger, Clyde Hutchinson, Andrew Cunningham, Debbie Pain, Richard Cuthbert, Andrea Raab, Andrew Alexander Meharg, Gerry Swan, Yadvendradev Jhala, Vibhu Prakash & 3 others Asad Rahmani, Miguel Quevedo, Reinhard Niessner

Research output: Chapter in Book/Report/Conference proceedingConference contribution

12 Citations (Scopus)

Abstract

A highly sensitive and specific competitive ELISA on 96-microwell plates was developed for the analysis of the nonsteroidal anti-inflammatory drug diclofenac. Within the water cycle in Europe, this is one of the most frequently detected pharmaceutically active compounds. The LOD at a signal-to-noise ratio (S/N) of 3, and the IC50, were found to be 6 ng/L and 60 ng/L respectively in tap water. In a comparative study using ELISA and GC-MS, diclofenac levels in wastewater from 21 sewage treatment plants were determined and a good correlation between these methods was found (ELISA vs. GC-MS: r = 0.70, slope = 0.90, intercept = 0.37, n = 24). An average degradation rate of approximate to 25% can be calculated. Lab-scale-experiments on the elimination of diclofenac in continuous pilot sewage plants revealed a removal rate of only 5% over a period of 13 weeks. In a further study, the ELISA was applied to a number of extracts of various animal tissues from a range of species, and again a very good relationship between ELISA and LC-ESI/MS data sets was obtained (r 0.90, p < 0.0001; n = 117). The ELISA has proven to be a simple, rapid, reliable and affordable alternative to otherwise costly and advanced techniques for the detection of diclofenac in matrix diverse water samples and tissue extracts after only relatively simple sample preparation.

Original languageEnglish
Title of host publicationRational Environmental Management of Agrochemicals
Subtitle of host publicationRisk Assessment, Monitoring, and Remedial Action
EditorsIvan R. Kennedy, Keith R. Solomon, Shirley J. Gee, Angus N. Crossan, Shuo Wang, Francisco Sánchez-Bayo
Place of PublicationWashington D.C.
PublisherAmerican Chemical Society
Pages203-226
Number of pages24
ISBN (Electronic)9780841220874
ISBN (Print)9780841274204
DOIs
Publication statusPublished - 2007

Publication series

NameACS symposium series
Volume966

Keywords

  • antiinflammatory drug diclofenac
  • waste-water treatment
  • aquatic environment
  • mass-spectrometry
  • clofibric acid
  • gyps-bengalensis
  • rainbow-trout
  • part 1
  • sewage
  • fate

Cite this

Knopp, D., Deng, A., Letzel, M., Taggart, M., Himmelsbach, M., Zhu, Q-Z., ... Niessner, R. (2007). Immunological Determination of the Pharmaceutical Diclofenac in Environmental and Biological Samples. In I. R. Kennedy, K. R. Solomon, S. J. Gee, A. N. Crossan, S. Wang, & F. Sánchez-Bayo (Eds.), Rational Environmental Management of Agrochemicals: Risk Assessment, Monitoring, and Remedial Action (pp. 203-226). (ACS symposium series; Vol. 966). Washington D.C.: American Chemical Society. https://doi.org/10.1021/bk-2007-0966.ch013

Immunological Determination of the Pharmaceutical Diclofenac in Environmental and Biological Samples. / Knopp, Dietmar; Deng, Anping; Letzel, Marion; Taggart, Mark; Himmelsbach, Markus; Zhu, Qing-Zhi; Peröbner, Iris; Kudlak, Blazej; Frey, Siegfried; Sengl, Manfred; Buchberger, Wolfgang; Hutchinson, Clyde; Cunningham, Andrew; Pain, Debbie; Cuthbert, Richard; Raab, Andrea; Meharg, Andrew Alexander; Swan, Gerry; Jhala, Yadvendradev; Prakash, Vibhu; Rahmani, Asad; Quevedo, Miguel; Niessner, Reinhard.

Rational Environmental Management of Agrochemicals: Risk Assessment, Monitoring, and Remedial Action. ed. / Ivan R. Kennedy; Keith R. Solomon; Shirley J. Gee; Angus N. Crossan; Shuo Wang; Francisco Sánchez-Bayo. Washington D.C. : American Chemical Society, 2007. p. 203-226 (ACS symposium series; Vol. 966).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Knopp, D, Deng, A, Letzel, M, Taggart, M, Himmelsbach, M, Zhu, Q-Z, Peröbner, I, Kudlak, B, Frey, S, Sengl, M, Buchberger, W, Hutchinson, C, Cunningham, A, Pain, D, Cuthbert, R, Raab, A, Meharg, AA, Swan, G, Jhala, Y, Prakash, V, Rahmani, A, Quevedo, M & Niessner, R 2007, Immunological Determination of the Pharmaceutical Diclofenac in Environmental and Biological Samples. in IR Kennedy, KR Solomon, SJ Gee, AN Crossan, S Wang & F Sánchez-Bayo (eds), Rational Environmental Management of Agrochemicals: Risk Assessment, Monitoring, and Remedial Action. ACS symposium series, vol. 966, American Chemical Society, Washington D.C., pp. 203-226. https://doi.org/10.1021/bk-2007-0966.ch013
Knopp D, Deng A, Letzel M, Taggart M, Himmelsbach M, Zhu Q-Z et al. Immunological Determination of the Pharmaceutical Diclofenac in Environmental and Biological Samples. In Kennedy IR, Solomon KR, Gee SJ, Crossan AN, Wang S, Sánchez-Bayo F, editors, Rational Environmental Management of Agrochemicals: Risk Assessment, Monitoring, and Remedial Action. Washington D.C.: American Chemical Society. 2007. p. 203-226. (ACS symposium series). https://doi.org/10.1021/bk-2007-0966.ch013
Knopp, Dietmar ; Deng, Anping ; Letzel, Marion ; Taggart, Mark ; Himmelsbach, Markus ; Zhu, Qing-Zhi ; Peröbner, Iris ; Kudlak, Blazej ; Frey, Siegfried ; Sengl, Manfred ; Buchberger, Wolfgang ; Hutchinson, Clyde ; Cunningham, Andrew ; Pain, Debbie ; Cuthbert, Richard ; Raab, Andrea ; Meharg, Andrew Alexander ; Swan, Gerry ; Jhala, Yadvendradev ; Prakash, Vibhu ; Rahmani, Asad ; Quevedo, Miguel ; Niessner, Reinhard. / Immunological Determination of the Pharmaceutical Diclofenac in Environmental and Biological Samples. Rational Environmental Management of Agrochemicals: Risk Assessment, Monitoring, and Remedial Action. editor / Ivan R. Kennedy ; Keith R. Solomon ; Shirley J. Gee ; Angus N. Crossan ; Shuo Wang ; Francisco Sánchez-Bayo. Washington D.C. : American Chemical Society, 2007. pp. 203-226 (ACS symposium series).
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AU - Knopp, Dietmar

AU - Deng, Anping

AU - Letzel, Marion

AU - Taggart, Mark

AU - Himmelsbach, Markus

AU - Zhu, Qing-Zhi

AU - Peröbner, Iris

AU - Kudlak, Blazej

AU - Frey, Siegfried

AU - Sengl, Manfred

AU - Buchberger, Wolfgang

AU - Hutchinson, Clyde

AU - Cunningham, Andrew

AU - Pain, Debbie

AU - Cuthbert, Richard

AU - Raab, Andrea

AU - Meharg, Andrew Alexander

AU - Swan, Gerry

AU - Jhala, Yadvendradev

AU - Prakash, Vibhu

AU - Rahmani, Asad

AU - Quevedo, Miguel

AU - Niessner, Reinhard

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N2 - A highly sensitive and specific competitive ELISA on 96-microwell plates was developed for the analysis of the nonsteroidal anti-inflammatory drug diclofenac. Within the water cycle in Europe, this is one of the most frequently detected pharmaceutically active compounds. The LOD at a signal-to-noise ratio (S/N) of 3, and the IC50, were found to be 6 ng/L and 60 ng/L respectively in tap water. In a comparative study using ELISA and GC-MS, diclofenac levels in wastewater from 21 sewage treatment plants were determined and a good correlation between these methods was found (ELISA vs. GC-MS: r = 0.70, slope = 0.90, intercept = 0.37, n = 24). An average degradation rate of approximate to 25% can be calculated. Lab-scale-experiments on the elimination of diclofenac in continuous pilot sewage plants revealed a removal rate of only 5% over a period of 13 weeks. In a further study, the ELISA was applied to a number of extracts of various animal tissues from a range of species, and again a very good relationship between ELISA and LC-ESI/MS data sets was obtained (r 0.90, p < 0.0001; n = 117). The ELISA has proven to be a simple, rapid, reliable and affordable alternative to otherwise costly and advanced techniques for the detection of diclofenac in matrix diverse water samples and tissue extracts after only relatively simple sample preparation.

AB - A highly sensitive and specific competitive ELISA on 96-microwell plates was developed for the analysis of the nonsteroidal anti-inflammatory drug diclofenac. Within the water cycle in Europe, this is one of the most frequently detected pharmaceutically active compounds. The LOD at a signal-to-noise ratio (S/N) of 3, and the IC50, were found to be 6 ng/L and 60 ng/L respectively in tap water. In a comparative study using ELISA and GC-MS, diclofenac levels in wastewater from 21 sewage treatment plants were determined and a good correlation between these methods was found (ELISA vs. GC-MS: r = 0.70, slope = 0.90, intercept = 0.37, n = 24). An average degradation rate of approximate to 25% can be calculated. Lab-scale-experiments on the elimination of diclofenac in continuous pilot sewage plants revealed a removal rate of only 5% over a period of 13 weeks. In a further study, the ELISA was applied to a number of extracts of various animal tissues from a range of species, and again a very good relationship between ELISA and LC-ESI/MS data sets was obtained (r 0.90, p < 0.0001; n = 117). The ELISA has proven to be a simple, rapid, reliable and affordable alternative to otherwise costly and advanced techniques for the detection of diclofenac in matrix diverse water samples and tissue extracts after only relatively simple sample preparation.

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KW - waste-water treatment

KW - aquatic environment

KW - mass-spectrometry

KW - clofibric acid

KW - gyps-bengalensis

KW - rainbow-trout

KW - part 1

KW - sewage

KW - fate

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BT - Rational Environmental Management of Agrochemicals

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