Immunomethods for detecting a broad range of polychlorinated biphenyls

Stephen A. Shelton, Barbara M. Graham, Gillian Strachan, Helen Dooley, Andrew J. Porter, William J. Harris*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Aroclor 1254 (consisting of a mixture of over 100 different congeners of polychlorinated biphenyls (PCBs) was adsorbed on to the surface of polystyrene microbeads and used to immunize mice and rabbits. High titre antisera were produced. PCBs were also adsorbed on to antimony pentoxide microbeads which were then used effectively as immobilized antigen in microbead-based ELISA assays. Antisera raised with Aroclor 1254-coated beads contained antibodies with high affinity (as determined by BIAcore analysis) against tetracholoro-biphenyls even though these congeners represent only a minor fraction in Aroclors.

Original languageEnglish
Pages (from-to)37-45
Number of pages9
JournalFood and Agricultural Immunology
Volume10
Issue number1
DOIs
Publication statusPublished - 1 Jan 1998

Keywords

  • ELISA
  • Immune sera
  • Immunoassay
  • PCBs

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