Immunomethods for detecting a broad range of polychlorinated biphenyls

Stephen A. Shelton; Barbara M. Graham; Gillian Strachan; Helen Dooley; Andrew J. Porter; William J. Harris

doi:10.1080/09540109809354967

Immunomethods for detecting a broad range of polychlorinated biphenyls

Stephen A. Shelton, Barbara M. Graham, Gillian Strachan, Helen Dooley, Andrew J. Porter, William J. Harris^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Aroclor 1254 (consisting of a mixture of over 100 different congeners of polychlorinated biphenyls (PCBs) was adsorbed on to the surface of polystyrene microbeads and used to immunize mice and rabbits. High titre antisera were produced. PCBs were also adsorbed on to antimony pentoxide microbeads which were then used effectively as immobilized antigen in microbead-based ELISA assays. Antisera raised with Aroclor 1254-coated beads contained antibodies with high affinity (as determined by BIAcore analysis) against tetracholoro-biphenyls even though these congeners represent only a minor fraction in Aroclors.

Original language	English
Pages (from-to)	37-45
Number of pages	9
Journal	Food and Agricultural Immunology
Volume	10
Issue number	1
DOIs	https://doi.org/10.1080/09540109809354967
Publication status	Published - 1 Jan 1998

Keywords

ELISA
Immune sera
Immunoassay
PCBs

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title = "Immunomethods for detecting a broad range of polychlorinated biphenyls",

abstract = "Aroclor 1254 (consisting of a mixture of over 100 different congeners of polychlorinated biphenyls (PCBs) was adsorbed on to the surface of polystyrene microbeads and used to immunize mice and rabbits. High titre antisera were produced. PCBs were also adsorbed on to antimony pentoxide microbeads which were then used effectively as immobilized antigen in microbead-based ELISA assays. Antisera raised with Aroclor 1254-coated beads contained antibodies with high affinity (as determined by BIAcore analysis) against tetracholoro-biphenyls even though these congeners represent only a minor fraction in Aroclors.",

keywords = "ELISA, Immune sera, Immunoassay, PCBs",

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AU - Shelton, Stephen A.

AU - Graham, Barbara M.

AU - Strachan, Gillian

AU - Dooley, Helen

AU - Porter, Andrew J.

AU - Harris, William J.

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Aroclor 1254 (consisting of a mixture of over 100 different congeners of polychlorinated biphenyls (PCBs) was adsorbed on to the surface of polystyrene microbeads and used to immunize mice and rabbits. High titre antisera were produced. PCBs were also adsorbed on to antimony pentoxide microbeads which were then used effectively as immobilized antigen in microbead-based ELISA assays. Antisera raised with Aroclor 1254-coated beads contained antibodies with high affinity (as determined by BIAcore analysis) against tetracholoro-biphenyls even though these congeners represent only a minor fraction in Aroclors.

AB - Aroclor 1254 (consisting of a mixture of over 100 different congeners of polychlorinated biphenyls (PCBs) was adsorbed on to the surface of polystyrene microbeads and used to immunize mice and rabbits. High titre antisera were produced. PCBs were also adsorbed on to antimony pentoxide microbeads which were then used effectively as immobilized antigen in microbead-based ELISA assays. Antisera raised with Aroclor 1254-coated beads contained antibodies with high affinity (as determined by BIAcore analysis) against tetracholoro-biphenyls even though these congeners represent only a minor fraction in Aroclors.

KW - ELISA

KW - Immune sera

KW - Immunoassay

KW - PCBs

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DO - 10.1080/09540109809354967

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EP - 45

JO - Food and Agricultural Immunology

JF - Food and Agricultural Immunology

IS - 1

ER -

Immunomethods for detecting a broad range of polychlorinated biphenyls

Abstract

Keywords

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