Immunoprophylaxis in fish by injection of mouse antibody genes

N  Lorenzen; P M  Cupit; K  Einer-Jensen; E  Lorenzen; P  Ahrens; C J  Secombes; C  Cunningham

Immunoprophylaxis in fish by injection of mouse antibody genes

N Lorenzen, P M Cupit, K Einer-Jensen, E Lorenzen, P Ahrens, C J Secombes, C Cunningham

Aberdeen Centre For Environmental Sustainability

Research output: Contribution to journal › Article

28 Citations (Scopus)

Abstract

Antibodies are a crucial part of the body's specific defense against infectious diseases and have considerable potential as therapeutic and prophylactic agents in humans and animals, The development of recombinant single-chain antibodies allows a genetic application strategy for prevention of infectious diseases. To test this in a fish model, a gene construct encoding a neutralizing single-chain antibody to the fish-pathogenic rhabdovirus VHSV (viral hemorrhagic septicemia virus) was administered to rainbow trout by intramuscular injection of plasmid DNA, Circulating recombinant antibodies could later be detected in the fish, and protective immunity to the viral disease was established.

Original language	English
Pages (from-to)	1177-1180
Number of pages	4
Journal	Nature Biotechnology
Volume	18
Publication status	Published - 2000

Keywords

single-chain antibody
rhabdovirus
gene therapy
SINGLE-CHAIN ANTIBODY
TROUT ONCORHYNCHUS-MYKISS
RAINBOW-TROUT
INTRACELLULAR EXPRESSION
MONOCLONAL-ANTIBODIES
PROTECTIVE IMMUNITY
LETHAL INFECTION
DNA VACCINES
FV FRAGMENTS
VIRUS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Immunoprophylaxis in fish by injection of mouse antibody genes",

abstract = "Antibodies are a crucial part of the body's specific defense against infectious diseases and have considerable potential as therapeutic and prophylactic agents in humans and animals, The development of recombinant single-chain antibodies allows a genetic application strategy for prevention of infectious diseases. To test this in a fish model, a gene construct encoding a neutralizing single-chain antibody to the fish-pathogenic rhabdovirus VHSV (viral hemorrhagic septicemia virus) was administered to rainbow trout by intramuscular injection of plasmid DNA, Circulating recombinant antibodies could later be detected in the fish, and protective immunity to the viral disease was established.",

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author = "N Lorenzen and Cupit, {P M} and K Einer-Jensen and E Lorenzen and P Ahrens and Secombes, {C J} and C Cunningham",

year = "2000",

language = "English",

volume = "18",

pages = "1177--1180",

journal = "Nature Biotechnology",

issn = "1087-0156",

publisher = "Nature Publishing Group",

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T1 - Immunoprophylaxis in fish by injection of mouse antibody genes

AU - Lorenzen, N

AU - Cupit, P M

AU - Einer-Jensen, K

AU - Lorenzen, E

AU - Ahrens, P

AU - Secombes, C J

AU - Cunningham, C

PY - 2000

Y1 - 2000

N2 - Antibodies are a crucial part of the body's specific defense against infectious diseases and have considerable potential as therapeutic and prophylactic agents in humans and animals, The development of recombinant single-chain antibodies allows a genetic application strategy for prevention of infectious diseases. To test this in a fish model, a gene construct encoding a neutralizing single-chain antibody to the fish-pathogenic rhabdovirus VHSV (viral hemorrhagic septicemia virus) was administered to rainbow trout by intramuscular injection of plasmid DNA, Circulating recombinant antibodies could later be detected in the fish, and protective immunity to the viral disease was established.

AB - Antibodies are a crucial part of the body's specific defense against infectious diseases and have considerable potential as therapeutic and prophylactic agents in humans and animals, The development of recombinant single-chain antibodies allows a genetic application strategy for prevention of infectious diseases. To test this in a fish model, a gene construct encoding a neutralizing single-chain antibody to the fish-pathogenic rhabdovirus VHSV (viral hemorrhagic septicemia virus) was administered to rainbow trout by intramuscular injection of plasmid DNA, Circulating recombinant antibodies could later be detected in the fish, and protective immunity to the viral disease was established.

KW - single-chain antibody

KW - rhabdovirus

KW - gene therapy

KW - SINGLE-CHAIN ANTIBODY

KW - TROUT ONCORHYNCHUS-MYKISS

KW - RAINBOW-TROUT

KW - INTRACELLULAR EXPRESSION

KW - MONOCLONAL-ANTIBODIES

KW - PROTECTIVE IMMUNITY

KW - LETHAL INFECTION

KW - DNA VACCINES

KW - FV FRAGMENTS

KW - VIRUS

M3 - Article

SN - 1087-0156

VL - 18

SP - 1177

EP - 1180

JO - Nature Biotechnology

JF - Nature Biotechnology

ER -

Immunoprophylaxis in fish by injection of mouse antibody genes

Abstract

Keywords

UN SDGs

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