Immunoprophylaxis in fish by injection of mouse antibody genes

N Lorenzen, P M Cupit, K Einer-Jensen, E Lorenzen, P Ahrens, C J Secombes, C Cunningham

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Antibodies are a crucial part of the body's specific defense against infectious diseases and have considerable potential as therapeutic and prophylactic agents in humans and animals, The development of recombinant single-chain antibodies allows a genetic application strategy for prevention of infectious diseases. To test this in a fish model, a gene construct encoding a neutralizing single-chain antibody to the fish-pathogenic rhabdovirus VHSV (viral hemorrhagic septicemia virus) was administered to rainbow trout by intramuscular injection of plasmid DNA, Circulating recombinant antibodies could later be detected in the fish, and protective immunity to the viral disease was established.

Original languageEnglish
Pages (from-to)1177-1180
Number of pages4
JournalNature Biotechnology
Volume18
Publication statusPublished - 2000

Keywords

  • single-chain antibody
  • rhabdovirus
  • gene therapy
  • SINGLE-CHAIN ANTIBODY
  • TROUT ONCORHYNCHUS-MYKISS
  • RAINBOW-TROUT
  • INTRACELLULAR EXPRESSION
  • MONOCLONAL-ANTIBODIES
  • PROTECTIVE IMMUNITY
  • LETHAL INFECTION
  • DNA VACCINES
  • FV FRAGMENTS
  • VIRUS

Cite this

Lorenzen, N., Cupit, P. M., Einer-Jensen, K., Lorenzen, E., Ahrens, P., Secombes, C. J., & Cunningham, C. (2000). Immunoprophylaxis in fish by injection of mouse antibody genes. Nature Biotechnology, 18, 1177-1180.