Immunoprophylaxis in fish by injection of mouse antibody genes

N Lorenzen, P M Cupit, K Einer-Jensen, E Lorenzen, P Ahrens, C J Secombes, C Cunningham

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Antibodies are a crucial part of the body's specific defense against infectious diseases and have considerable potential as therapeutic and prophylactic agents in humans and animals, The development of recombinant single-chain antibodies allows a genetic application strategy for prevention of infectious diseases. To test this in a fish model, a gene construct encoding a neutralizing single-chain antibody to the fish-pathogenic rhabdovirus VHSV (viral hemorrhagic septicemia virus) was administered to rainbow trout by intramuscular injection of plasmid DNA, Circulating recombinant antibodies could later be detected in the fish, and protective immunity to the viral disease was established.

Original languageEnglish
Pages (from-to)1177-1180
Number of pages4
JournalNature Biotechnology
Volume18
Publication statusPublished - 2000

Keywords

  • single-chain antibody
  • rhabdovirus
  • gene therapy
  • SINGLE-CHAIN ANTIBODY
  • TROUT ONCORHYNCHUS-MYKISS
  • RAINBOW-TROUT
  • INTRACELLULAR EXPRESSION
  • MONOCLONAL-ANTIBODIES
  • PROTECTIVE IMMUNITY
  • LETHAL INFECTION
  • DNA VACCINES
  • FV FRAGMENTS
  • VIRUS

Cite this

Lorenzen, N., Cupit, P. M., Einer-Jensen, K., Lorenzen, E., Ahrens, P., Secombes, C. J., & Cunningham, C. (2000). Immunoprophylaxis in fish by injection of mouse antibody genes. Nature Biotechnology, 18, 1177-1180.

Immunoprophylaxis in fish by injection of mouse antibody genes. / Lorenzen, N ; Cupit, P M ; Einer-Jensen, K ; Lorenzen, E ; Ahrens, P ; Secombes, C J ; Cunningham, C .

In: Nature Biotechnology, Vol. 18, 2000, p. 1177-1180.

Research output: Contribution to journalArticle

Lorenzen, N, Cupit, PM, Einer-Jensen, K, Lorenzen, E, Ahrens, P, Secombes, CJ & Cunningham, C 2000, 'Immunoprophylaxis in fish by injection of mouse antibody genes', Nature Biotechnology, vol. 18, pp. 1177-1180.
Lorenzen N, Cupit PM, Einer-Jensen K, Lorenzen E, Ahrens P, Secombes CJ et al. Immunoprophylaxis in fish by injection of mouse antibody genes. Nature Biotechnology. 2000;18:1177-1180.
Lorenzen, N ; Cupit, P M ; Einer-Jensen, K ; Lorenzen, E ; Ahrens, P ; Secombes, C J ; Cunningham, C . / Immunoprophylaxis in fish by injection of mouse antibody genes. In: Nature Biotechnology. 2000 ; Vol. 18. pp. 1177-1180.
@article{c9fd264221bc4d5fb6a87a2b40f75f6b,
title = "Immunoprophylaxis in fish by injection of mouse antibody genes",
abstract = "Antibodies are a crucial part of the body's specific defense against infectious diseases and have considerable potential as therapeutic and prophylactic agents in humans and animals, The development of recombinant single-chain antibodies allows a genetic application strategy for prevention of infectious diseases. To test this in a fish model, a gene construct encoding a neutralizing single-chain antibody to the fish-pathogenic rhabdovirus VHSV (viral hemorrhagic septicemia virus) was administered to rainbow trout by intramuscular injection of plasmid DNA, Circulating recombinant antibodies could later be detected in the fish, and protective immunity to the viral disease was established.",
keywords = "single-chain antibody, rhabdovirus, gene therapy, SINGLE-CHAIN ANTIBODY, TROUT ONCORHYNCHUS-MYKISS, RAINBOW-TROUT, INTRACELLULAR EXPRESSION, MONOCLONAL-ANTIBODIES, PROTECTIVE IMMUNITY, LETHAL INFECTION, DNA VACCINES, FV FRAGMENTS, VIRUS",
author = "N Lorenzen and Cupit, {P M} and K Einer-Jensen and E Lorenzen and P Ahrens and Secombes, {C J} and C Cunningham",
year = "2000",
language = "English",
volume = "18",
pages = "1177--1180",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Immunoprophylaxis in fish by injection of mouse antibody genes

AU - Lorenzen, N

AU - Cupit, P M

AU - Einer-Jensen, K

AU - Lorenzen, E

AU - Ahrens, P

AU - Secombes, C J

AU - Cunningham, C

PY - 2000

Y1 - 2000

N2 - Antibodies are a crucial part of the body's specific defense against infectious diseases and have considerable potential as therapeutic and prophylactic agents in humans and animals, The development of recombinant single-chain antibodies allows a genetic application strategy for prevention of infectious diseases. To test this in a fish model, a gene construct encoding a neutralizing single-chain antibody to the fish-pathogenic rhabdovirus VHSV (viral hemorrhagic septicemia virus) was administered to rainbow trout by intramuscular injection of plasmid DNA, Circulating recombinant antibodies could later be detected in the fish, and protective immunity to the viral disease was established.

AB - Antibodies are a crucial part of the body's specific defense against infectious diseases and have considerable potential as therapeutic and prophylactic agents in humans and animals, The development of recombinant single-chain antibodies allows a genetic application strategy for prevention of infectious diseases. To test this in a fish model, a gene construct encoding a neutralizing single-chain antibody to the fish-pathogenic rhabdovirus VHSV (viral hemorrhagic septicemia virus) was administered to rainbow trout by intramuscular injection of plasmid DNA, Circulating recombinant antibodies could later be detected in the fish, and protective immunity to the viral disease was established.

KW - single-chain antibody

KW - rhabdovirus

KW - gene therapy

KW - SINGLE-CHAIN ANTIBODY

KW - TROUT ONCORHYNCHUS-MYKISS

KW - RAINBOW-TROUT

KW - INTRACELLULAR EXPRESSION

KW - MONOCLONAL-ANTIBODIES

KW - PROTECTIVE IMMUNITY

KW - LETHAL INFECTION

KW - DNA VACCINES

KW - FV FRAGMENTS

KW - VIRUS

M3 - Article

VL - 18

SP - 1177

EP - 1180

JO - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

ER -