Immunostimulatory CpG oligodeoxynucleotides stimulate expression of IL-1 beta and interferon-like cytokines in rainbow trout macrophages via a chloroquine-sensitive mechanism

J B Jorgensen, Jun Zou, A Johansen, Christopher John Secombes

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs are known to stimulate immune responses and are potent adjuvant's in higher vertebrates, but so far the effects in fish are poorly described. We here report that CpG ODNs induce IL-1 beta expression and production of interferon-like cytokines in rainbow trout head-kidney macrophages, whereas ODNs with an inverted motif (GpC) have a much less stimulatory effect. We further demonstrate that endosomal maturation is essential for CpG signalling, as chloroquine, a compound known to block endosomal acidification, inhibits cytokine expression in the macrophages. (C) 2001 Academic Press.

Original languageEnglish
Pages (from-to)673-682
Number of pages10
JournalFish & Shellfish Immunology
Volume11
Issue number8
DOIs
Publication statusPublished - Nov 2001

Keywords

  • rainbow trout
  • CpG motif
  • cytokine
  • interferon
  • IL-1 beta
  • macrophages
  • bacterial-DNA
  • immune-response
  • innate immunity
  • B-cell
  • motifs
  • adjuvants
  • oligonecleotides
  • activation
  • vaccines
  • antigen

Cite this

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title = "Immunostimulatory CpG oligodeoxynucleotides stimulate expression of IL-1 beta and interferon-like cytokines in rainbow trout macrophages via a chloroquine-sensitive mechanism",
abstract = "Synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs are known to stimulate immune responses and are potent adjuvant's in higher vertebrates, but so far the effects in fish are poorly described. We here report that CpG ODNs induce IL-1 beta expression and production of interferon-like cytokines in rainbow trout head-kidney macrophages, whereas ODNs with an inverted motif (GpC) have a much less stimulatory effect. We further demonstrate that endosomal maturation is essential for CpG signalling, as chloroquine, a compound known to block endosomal acidification, inhibits cytokine expression in the macrophages. (C) 2001 Academic Press.",
keywords = "rainbow trout, CpG motif, cytokine, interferon, IL-1 beta, macrophages, bacterial-DNA, immune-response, innate immunity, B-cell, motifs, adjuvants, oligonecleotides, activation, vaccines, antigen",
author = "Jorgensen, {J B} and Jun Zou and A Johansen and Secombes, {Christopher John}",
year = "2001",
month = "11",
doi = "10.1006/fsim.2001.0344",
language = "English",
volume = "11",
pages = "673--682",
journal = "Fish & Shellfish Immunology",
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publisher = "ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD",
number = "8",

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TY - JOUR

T1 - Immunostimulatory CpG oligodeoxynucleotides stimulate expression of IL-1 beta and interferon-like cytokines in rainbow trout macrophages via a chloroquine-sensitive mechanism

AU - Jorgensen, J B

AU - Zou, Jun

AU - Johansen, A

AU - Secombes, Christopher John

PY - 2001/11

Y1 - 2001/11

N2 - Synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs are known to stimulate immune responses and are potent adjuvant's in higher vertebrates, but so far the effects in fish are poorly described. We here report that CpG ODNs induce IL-1 beta expression and production of interferon-like cytokines in rainbow trout head-kidney macrophages, whereas ODNs with an inverted motif (GpC) have a much less stimulatory effect. We further demonstrate that endosomal maturation is essential for CpG signalling, as chloroquine, a compound known to block endosomal acidification, inhibits cytokine expression in the macrophages. (C) 2001 Academic Press.

AB - Synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs are known to stimulate immune responses and are potent adjuvant's in higher vertebrates, but so far the effects in fish are poorly described. We here report that CpG ODNs induce IL-1 beta expression and production of interferon-like cytokines in rainbow trout head-kidney macrophages, whereas ODNs with an inverted motif (GpC) have a much less stimulatory effect. We further demonstrate that endosomal maturation is essential for CpG signalling, as chloroquine, a compound known to block endosomal acidification, inhibits cytokine expression in the macrophages. (C) 2001 Academic Press.

KW - rainbow trout

KW - CpG motif

KW - cytokine

KW - interferon

KW - IL-1 beta

KW - macrophages

KW - bacterial-DNA

KW - immune-response

KW - innate immunity

KW - B-cell

KW - motifs

KW - adjuvants

KW - oligonecleotides

KW - activation

KW - vaccines

KW - antigen

U2 - 10.1006/fsim.2001.0344

DO - 10.1006/fsim.2001.0344

M3 - Article

VL - 11

SP - 673

EP - 682

JO - Fish & Shellfish Immunology

JF - Fish & Shellfish Immunology

SN - 1050-4648

IS - 8

ER -