Impact of birth weight and gender on early postnatal hypothalamic energy balance regulatory gene expression in the young lamb

C. L. Adam, T. Bake, P. A. Findlay, J. S. Milne, R. P. Aitken, J. M. Wallace

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Intra-uterine growth restriction (IUGR) is involved in developmental metabolic programming and here we test the hypothesis that IUGR affects the developing hypothalamic energy balance regulatory pathways in a sex-specific manner. This experiment investigated early postnatal hypothalamic gene expression for six primary leptin- and insulin-sensitive neuropeptides and receptors in male and female IUGR (n = 8 and 9, respectively) and normal (N) birth weight lambs (n = 8 per gender) gestated and suckled by overnourished mothers. IUGR lambs were smaller at birth, had increased fractional growth rates (FGR), lower final body weight (11 weeks) and similar body fat content compared with N lambs, while males had higher final body weight and insulinemia but lower body fat and leptinemia than females. In situ hybridization revealed greater gene expression in the hypothalamic arcuate nucleus at 11 weeks for anorexigenic genes in females and orexigenic genes in males, with no effect of IUGR. Leptinemia correlated with gene expression for neuropeptide Y (NPY, negatively) in both sexes and pro-opiomelanocortin (POMC, positively) in females but with leptin receptor (negatively) only in males. Current FGR for girth correlated negatively with gene expression for NPY in males and POMC in females. Neither IUGR nor gender affected suckling activity (proxy for appetite) assessed at 3 weeks, but final NPY gene expression correlated with suckling weight gain in males. This study has revealed no effect of IUGR on early postnatal hypothalamic energy balance gene expression but a major effect of gender associated with major sex differences in adiposity and leptinemia.
Original languageEnglish
Pages (from-to)608-615
Number of pages8
JournalInternational Journal of Developmental Neuroscience
Volume31
Issue number7
DOIs
Publication statusPublished - Nov 2013

Fingerprint

Regulator Genes
Birth Weight
Gene Expression
Growth
Pro-Opiomelanocortin
Adipose Tissue
Body Weight
Neuropeptide Receptors
Leptin Receptors
Arcuate Nucleus of Hypothalamus
Neuropeptide Y
Adiposity
Proxy
Appetite
Leptin
Sex Characteristics
Genes
Weight Gain
In Situ Hybridization
Mothers

Keywords

  • developmental programming
  • intra-uterine growth restriction
  • adiposity
  • leptin

Cite this

Impact of birth weight and gender on early postnatal hypothalamic energy balance regulatory gene expression in the young lamb. / Adam, C. L.; Bake, T.; Findlay, P. A.; Milne, J. S.; Aitken, R. P.; Wallace, J. M.

In: International Journal of Developmental Neuroscience, Vol. 31, No. 7, 11.2013, p. 608-615.

Research output: Contribution to journalArticle

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