Impact of carbohydrate substrate complexity on the diversity of the human colonic microbiota

Wing Sun Faith Chung, Alan W. Walker, Joan Vermeiren, Paul O. Sheridan, Douwina Bosscher, Vicenta Garcia-Campayo, Julian Parkhill, Harry J. Flint, Sylvia H. Duncan (Corresponding Author)

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The diversity of the colonic microbial community has been linked with health in adults and diet composition is one possible determinant of diversity. We used carefully controlled conditions in vitro to determine how the complexity and multiplicity of growth substrates influence species diversity of the human colonic microbiota. In each experiment, five parallel anaerobic fermentors that received identical faecal inocula were supplied continuously with single carbohydrates (either arabinoxylan-oligosaccharides (AXOS), pectin or inulin) or with a ‘3-mix’ of all three carbohydrates, or with a ‘6-mix’ that additionally contained resistant starch, β-glucan and galactomannan as energy sources. Inulin supported less microbial diversity over the first six days than the other two single substrates or the 3- and 6-mixes, showing that substrate complexity is key to influencing microbiota diversity. The communities enriched in these fermentors did not differ greatly at the phylum and family level, but were markedly different at the species level. Certain species were promoted by single substrates, whilst others (such as Bacteroides ovatus, LEfSe p=0.001) showed significantly greater success with the mixed substrate. The complex polysaccharides such as pectin and arabinoxylan-oligosaccharides promoted greater diversity than simple homopolymers, such as inulin. These findings suggest that dietary strategies intended to achieve health benefits by increasing gut microbiota diversity should employ complex non-digestible substrates and substrate mixtures.
Original languageEnglish
Article numberfiy201
JournalFEMS Microbiology Ecology
Volume95
Issue number1
Early online date9 Oct 2018
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Inulin
Microbiota
carbohydrate
Carbohydrates
Bioreactors
Oligosaccharides
substrate
Bacteroides
Glucans
Insurance Benefits
Starch
Polysaccharides
Diet
Health
Growth
polysaccharide
starch
microbial community
species diversity
diet

Keywords

  • microbial diversity
  • bacteroidetes
  • firmicutes
  • CAZymes
  • dietary carbohydrates

ASJC Scopus subject areas

  • Microbiology
  • Ecology
  • Applied Microbiology and Biotechnology

Cite this

Impact of carbohydrate substrate complexity on the diversity of the human colonic microbiota. / Chung, Wing Sun Faith; Walker, Alan W.; Vermeiren, Joan; Sheridan, Paul O.; Bosscher, Douwina; Garcia-Campayo, Vicenta; Parkhill, Julian; Flint, Harry J.; Duncan, Sylvia H. (Corresponding Author).

In: FEMS Microbiology Ecology, Vol. 95, No. 1, fiy201, 01.01.2019.

Research output: Contribution to journalArticle

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