In vitro activation of rainbow trout macrophages stimulates inhibition of Renibacterium salmoninarum growth concomitant with augmented generation of respiratory burst products

L. J. Hardie, A. E. Ellis, C. J. Secombes

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66 Citations (Scopus)

Abstract

Rainbow trout head kidney macrophages pretreated for 48 h with a supernatant which contained macrophage activating factor (MAF) obtained from Concanavalin A/phorbol myristate acetate stimulated rainbow trout leucocytes express an enhanced ability to inhibit Renibacterium salmoninarum growth in vitro compared with untreated controls. Enhanced 'killing' capacity of these macrophages was detectable after 24 h exposure to R. salmoninarum in vitro. Addition of N G -methyl-L-arginine in vitro did not diminish bacterial growth inhibition by MAF-activated macrophages, suggesting that nitric oxide radicals were not involved in this event. However, MAF-treated macrophages expressed enhanced respiratory burst activity compared with control macrophages after a 3 d exposure to R. salmoninarum in vitro. In addition, it was demonstrated that R. salmoninarum was susceptible to hydrogen peroxide (H 2 O 2 ) in vitro using a cell-free assay. Introduction of catalase to the in vitro macrophage assay abrogated the 'killing' activity, suggesting that H 2 O 2 release from activated macrophages was involved in the inhibition of R. salmoninarum growth. The results are discussed in relation to other intracellular pathogens and the possible importance of cell-mediated immunity for protection against bacterial kidney disease.

Original languageEnglish
Pages (from-to)175-183
Number of pages9
JournalDiseases of Aquatic Organisms
Volume25
Issue number3
DOIs
Publication statusPublished - 6 Jun 1996

Fingerprint

Renibacterium salmoninarum
rainbow
Oncorhynchus mykiss
macrophages
bacterial disease
nitric oxide
immunity
hydrogen peroxide
acetate
pathogen
assay
bacterial kidney disease
product
assays
concanavalin A
exposure
cell-mediated immunity
growth retardation
arginine
microbial growth

Keywords

  • Growth inhibition
  • Macrophage activation
  • Renibacterium salmoninarum
  • Respiratory burst

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

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abstract = "Rainbow trout head kidney macrophages pretreated for 48 h with a supernatant which contained macrophage activating factor (MAF) obtained from Concanavalin A/phorbol myristate acetate stimulated rainbow trout leucocytes express an enhanced ability to inhibit Renibacterium salmoninarum growth in vitro compared with untreated controls. Enhanced 'killing' capacity of these macrophages was detectable after 24 h exposure to R. salmoninarum in vitro. Addition of N G -methyl-L-arginine in vitro did not diminish bacterial growth inhibition by MAF-activated macrophages, suggesting that nitric oxide radicals were not involved in this event. However, MAF-treated macrophages expressed enhanced respiratory burst activity compared with control macrophages after a 3 d exposure to R. salmoninarum in vitro. In addition, it was demonstrated that R. salmoninarum was susceptible to hydrogen peroxide (H 2 O 2 ) in vitro using a cell-free assay. Introduction of catalase to the in vitro macrophage assay abrogated the 'killing' activity, suggesting that H 2 O 2 release from activated macrophages was involved in the inhibition of R. salmoninarum growth. The results are discussed in relation to other intracellular pathogens and the possible importance of cell-mediated immunity for protection against bacterial kidney disease.",
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AU - Ellis, A. E.

AU - Secombes, C. J.

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AB - Rainbow trout head kidney macrophages pretreated for 48 h with a supernatant which contained macrophage activating factor (MAF) obtained from Concanavalin A/phorbol myristate acetate stimulated rainbow trout leucocytes express an enhanced ability to inhibit Renibacterium salmoninarum growth in vitro compared with untreated controls. Enhanced 'killing' capacity of these macrophages was detectable after 24 h exposure to R. salmoninarum in vitro. Addition of N G -methyl-L-arginine in vitro did not diminish bacterial growth inhibition by MAF-activated macrophages, suggesting that nitric oxide radicals were not involved in this event. However, MAF-treated macrophages expressed enhanced respiratory burst activity compared with control macrophages after a 3 d exposure to R. salmoninarum in vitro. In addition, it was demonstrated that R. salmoninarum was susceptible to hydrogen peroxide (H 2 O 2 ) in vitro using a cell-free assay. Introduction of catalase to the in vitro macrophage assay abrogated the 'killing' activity, suggesting that H 2 O 2 release from activated macrophages was involved in the inhibition of R. salmoninarum growth. The results are discussed in relation to other intracellular pathogens and the possible importance of cell-mediated immunity for protection against bacterial kidney disease.

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