In vitro guidance of retinal axons by a tectal lamina-specific glycoprotein Nel

Yulan Jiang, Hiroya Obama, Soh Leh Kuan, Ritsuko Nakamura, Chizu Nakamoto, Zhufeng Ouyang, Masaru Nakamoto

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Abstract

Nel is a glycoprotein containing five chordin-like and six epidermal growth factor-like domains and is strongly expressed in the nervous system. In this study, we have examined expression patterns and in vitro functions of Nel in the chicken retinotectal system. We have found that in the developing tectum, expression of Nel is localized in specific laminae that retinal axons normally do not enter, including the border between the retinorecipient and non-retinorecipient laminae. Nel-binding activity is detected on retinal axons both in vivo and in vitro, suggesting that retinal axons express a receptor for Nel. In vitro, Nel inhibits retinal axon outgrowth and induces growth cone collapse and axon retraction. These results indicate that Nel acts as an inhibitory guidance cue for retinal axons, and suggest its roles in the establishment of the lamina-specificity in the retinotectal projection.
Nel is a glycoprotein containing five chordin-like and six epidermal growth factor-like domains and is strongly expressed in the nervous system. In this study, we have examined expression patterns and in vitro functions of Nel in the chicken retinotectal system. We have found that in the developing tectum, expression of Nel is localized in specific laminae that retinal axons normally do not enter, including the border between the retinorecipient and non-retinorecipient laminae. Nel-binding activity is detected on retinal axons both in vivo and in vitro, suggesting that retinal axons express a receptor for Nel. In vitro, Nel inhibits retinal axon outgrowth and induces growth cone collapse and axon retraction. These results indicate that Nel acts as an inhibitory guidance cue for retinal axons, and suggest its roles in the establishment of the lamina-specificity in the retinotectal projection.
Original languageEnglish
Pages (from-to)113-119
Number of pages7
JournalMolecular and Cellular Neuroscience
Volume41
Issue number2
Early online date26 Feb 2009
DOIs
Publication statusPublished - Jun 2009

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Axons
Glycoproteins
Growth Cones
Epidermal Growth Factor
Nervous System
Cues
Chickens
Axon Guidance
In Vitro Techniques

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Jiang, Y., Obama, H., Kuan, S. L., Nakamura, R., Nakamoto, C., Ouyang, Z., & Nakamoto, M. (2009). In vitro guidance of retinal axons by a tectal lamina-specific glycoprotein Nel. Molecular and Cellular Neuroscience, 41(2), 113-119. https://doi.org/10.1016/j.mcn.2009.02.006

In vitro guidance of retinal axons by a tectal lamina-specific glycoprotein Nel. / Jiang, Yulan; Obama, Hiroya; Kuan, Soh Leh; Nakamura, Ritsuko; Nakamoto, Chizu; Ouyang, Zhufeng; Nakamoto, Masaru.

In: Molecular and Cellular Neuroscience, Vol. 41, No. 2, 06.2009, p. 113-119.

Research output: Contribution to journalArticle

Jiang, Y, Obama, H, Kuan, SL, Nakamura, R, Nakamoto, C, Ouyang, Z & Nakamoto, M 2009, 'In vitro guidance of retinal axons by a tectal lamina-specific glycoprotein Nel' Molecular and Cellular Neuroscience, vol. 41, no. 2, pp. 113-119. https://doi.org/10.1016/j.mcn.2009.02.006
Jiang, Yulan ; Obama, Hiroya ; Kuan, Soh Leh ; Nakamura, Ritsuko ; Nakamoto, Chizu ; Ouyang, Zhufeng ; Nakamoto, Masaru. / In vitro guidance of retinal axons by a tectal lamina-specific glycoprotein Nel. In: Molecular and Cellular Neuroscience. 2009 ; Vol. 41, No. 2. pp. 113-119.
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