In vitro study of flavonoids, fatty acids, and steroids on proliferation of rat hepatic stellate cells

Farid A Badria, Abdel-Aziz A Dawidar, Wael E Houssen, Wayne T Shier

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

There is a wealth of evidence that hepatic stellate cells (HSCs) orchestrate most of the important events in liver fibrogenesis. After liver injury, HSCs become activated to a profibrogenic myofibroblastic phenotype and can regulate net deposition of collagens and other matrix proteins in the liver. The proliferation of HSCs is mainly stimulated by the platelet-derived growth factor (PDGF). In this study, some compounds from natural resources have been tested for their activity to inhibit PDGF-driven proliferative activity of rat HSCs. Apigenin, quercetin, genistein, daidzin, and biochanin A exhibited > 75% inhibitory activity against HSC-T6. It was found that, gamma-linolenic (gamma-Ln), eicosapentanoic (EPA) and a- linolenic (alpha-Ln) acids showed a high inhibitory effect on proliferation of rat HSCs at 50 nmol/1. Cholest-4-ene-3,6-dione and stigmastone-4-en-3,6-dione are the most active steroids with inhibitory activities > 80% and this is most likely due to the presence of the 4-en-3,6-dione moiety in both compounds. These results revealed that the compounds which effectively blocked HSC proliferation may be beneficial in liver fibrosis. Structure-activity relationships (SAR) may provide a basis for rational structure modification.
Original languageEnglish
Pages (from-to)139-42
Number of pages4
JournalZeitschrift für Naturforschung - C
Volume60
Issue number1-2
Publication statusPublished - 2005

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Hepatic Stellate Cells
Flavonoids
Rats
Fatty Acids
Steroids
Liver
Platelet-Derived Growth Factor
Apigenin
alpha-Linolenic Acid
Genistein
Quercetin
Cell proliferation
Natural resources
Structure-Activity Relationship
In Vitro Techniques
Liver Cirrhosis
Collagen
Cell Proliferation
Phenotype
Acids

Cite this

In vitro study of flavonoids, fatty acids, and steroids on proliferation of rat hepatic stellate cells. / Badria, Farid A; Dawidar, Abdel-Aziz A; Houssen, Wael E; Shier, Wayne T.

In: Zeitschrift für Naturforschung - C, Vol. 60, No. 1-2, 2005, p. 139-42.

Research output: Contribution to journalArticle

Badria, Farid A ; Dawidar, Abdel-Aziz A ; Houssen, Wael E ; Shier, Wayne T. / In vitro study of flavonoids, fatty acids, and steroids on proliferation of rat hepatic stellate cells. In: Zeitschrift für Naturforschung - C. 2005 ; Vol. 60, No. 1-2. pp. 139-42.
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AB - There is a wealth of evidence that hepatic stellate cells (HSCs) orchestrate most of the important events in liver fibrogenesis. After liver injury, HSCs become activated to a profibrogenic myofibroblastic phenotype and can regulate net deposition of collagens and other matrix proteins in the liver. The proliferation of HSCs is mainly stimulated by the platelet-derived growth factor (PDGF). In this study, some compounds from natural resources have been tested for their activity to inhibit PDGF-driven proliferative activity of rat HSCs. Apigenin, quercetin, genistein, daidzin, and biochanin A exhibited > 75% inhibitory activity against HSC-T6. It was found that, gamma-linolenic (gamma-Ln), eicosapentanoic (EPA) and a- linolenic (alpha-Ln) acids showed a high inhibitory effect on proliferation of rat HSCs at 50 nmol/1. Cholest-4-ene-3,6-dione and stigmastone-4-en-3,6-dione are the most active steroids with inhibitory activities > 80% and this is most likely due to the presence of the 4-en-3,6-dione moiety in both compounds. These results revealed that the compounds which effectively blocked HSC proliferation may be beneficial in liver fibrosis. Structure-activity relationships (SAR) may provide a basis for rational structure modification.

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