In Vivo Safety of Trypan Blue Use in Vitreoretinal Surgery

Ehab A. Abdelkader, Vikki A. McBain, Mrinal Anand, Neil W. Scott, M. A. Rehman Siddiqui, Noemi Lois

Research output: Contribution to journalArticle

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Abstract

Purpose: The purpose of this study was to evaluate "in vivo'' safety of trypan blue (TB) in patients undergoing TB-assisted internal limiting membrane or epiretinal membrane peeling.

Methods: Prospective study including 21 patients (21 eyes) with full-thickness macular hole and/or epiretinal membrane undergoing TB-assisted internal limiting membrane/epiretinal membrane peeling. Main outcome measures included distance visual acuity, near visual acuity, amplitude of P50 and N95 of the pattern electroretinogram, and fundus autofluorescence; these were assessed preoperatively, at 6 months (n = 21) and 12 months (n = 10) postoperatively.

Results: There was a statistically significant improvement in distance visual acuity, near visual acuity, P50, and N95 amplitude at 6 months and 12 months postoperatively. The mean logarithm of the minimum angle of resolution distance visual acuity and near visual acuity improved from baseline by 0.31 (SD 0.37) and 0.17 (SD 0.31) at 6 months, respectively, and by 0.4 (SD 0.25) and 0.35 (SD 0.28) at 12 months, respectively. The mean P50 and N95 component amplitudes improved by 28% compared with baseline at 6 months (P50 0.4 [SD 0.8]; N95 0.53 [SD 1.07]) and by 63% at 12 months (P50 0.9 [0.85]; N95 1.04 [1.34]). Autofluorescence did not demonstrate damage to the retinal pigment epithelium attributable to TB.

Conclusion: No deleterious effects of TB were observed in this study. RETINA 31:1122-1127, 2011

Original languageEnglish
Pages (from-to)1122-1127
Number of pages6
JournalRetina
Volume31
Issue number6
DOIs
Publication statusPublished - Jun 2011

Keywords

  • autofluorescence imaging
  • epiretinal membrane
  • macular hole
  • pattern electroretinogram
  • retinal function
  • trypan blue
  • internal limiting membrane
  • macular hole surgery
  • indocyanine green
  • infracyanine green
  • ISCEV standard
  • electroretinography
  • vitrectomy
  • histology
  • update

Cite this

In Vivo Safety of Trypan Blue Use in Vitreoretinal Surgery. / Abdelkader, Ehab A.; McBain, Vikki A.; Anand, Mrinal; Scott, Neil W.; Siddiqui, M. A. Rehman; Lois, Noemi.

In: Retina, Vol. 31, No. 6, 06.2011, p. 1122-1127.

Research output: Contribution to journalArticle

Abdelkader, Ehab A. ; McBain, Vikki A. ; Anand, Mrinal ; Scott, Neil W. ; Siddiqui, M. A. Rehman ; Lois, Noemi. / In Vivo Safety of Trypan Blue Use in Vitreoretinal Surgery. In: Retina. 2011 ; Vol. 31, No. 6. pp. 1122-1127.
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abstract = "Purpose: The purpose of this study was to evaluate {"}in vivo'' safety of trypan blue (TB) in patients undergoing TB-assisted internal limiting membrane or epiretinal membrane peeling.Methods: Prospective study including 21 patients (21 eyes) with full-thickness macular hole and/or epiretinal membrane undergoing TB-assisted internal limiting membrane/epiretinal membrane peeling. Main outcome measures included distance visual acuity, near visual acuity, amplitude of P50 and N95 of the pattern electroretinogram, and fundus autofluorescence; these were assessed preoperatively, at 6 months (n = 21) and 12 months (n = 10) postoperatively.Results: There was a statistically significant improvement in distance visual acuity, near visual acuity, P50, and N95 amplitude at 6 months and 12 months postoperatively. The mean logarithm of the minimum angle of resolution distance visual acuity and near visual acuity improved from baseline by 0.31 (SD 0.37) and 0.17 (SD 0.31) at 6 months, respectively, and by 0.4 (SD 0.25) and 0.35 (SD 0.28) at 12 months, respectively. The mean P50 and N95 component amplitudes improved by 28{\%} compared with baseline at 6 months (P50 0.4 [SD 0.8]; N95 0.53 [SD 1.07]) and by 63{\%} at 12 months (P50 0.9 [0.85]; N95 1.04 [1.34]). Autofluorescence did not demonstrate damage to the retinal pigment epithelium attributable to TB.Conclusion: No deleterious effects of TB were observed in this study. RETINA 31:1122-1127, 2011",
keywords = "autofluorescence imaging, epiretinal membrane, macular hole, pattern electroretinogram, retinal function, trypan blue, internal limiting membrane, macular hole surgery, indocyanine green, infracyanine green, ISCEV standard, electroretinography, vitrectomy, histology, update",
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T1 - In Vivo Safety of Trypan Blue Use in Vitreoretinal Surgery

AU - Abdelkader, Ehab A.

AU - McBain, Vikki A.

AU - Anand, Mrinal

AU - Scott, Neil W.

AU - Siddiqui, M. A. Rehman

AU - Lois, Noemi

PY - 2011/6

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N2 - Purpose: The purpose of this study was to evaluate "in vivo'' safety of trypan blue (TB) in patients undergoing TB-assisted internal limiting membrane or epiretinal membrane peeling.Methods: Prospective study including 21 patients (21 eyes) with full-thickness macular hole and/or epiretinal membrane undergoing TB-assisted internal limiting membrane/epiretinal membrane peeling. Main outcome measures included distance visual acuity, near visual acuity, amplitude of P50 and N95 of the pattern electroretinogram, and fundus autofluorescence; these were assessed preoperatively, at 6 months (n = 21) and 12 months (n = 10) postoperatively.Results: There was a statistically significant improvement in distance visual acuity, near visual acuity, P50, and N95 amplitude at 6 months and 12 months postoperatively. The mean logarithm of the minimum angle of resolution distance visual acuity and near visual acuity improved from baseline by 0.31 (SD 0.37) and 0.17 (SD 0.31) at 6 months, respectively, and by 0.4 (SD 0.25) and 0.35 (SD 0.28) at 12 months, respectively. The mean P50 and N95 component amplitudes improved by 28% compared with baseline at 6 months (P50 0.4 [SD 0.8]; N95 0.53 [SD 1.07]) and by 63% at 12 months (P50 0.9 [0.85]; N95 1.04 [1.34]). Autofluorescence did not demonstrate damage to the retinal pigment epithelium attributable to TB.Conclusion: No deleterious effects of TB were observed in this study. RETINA 31:1122-1127, 2011

AB - Purpose: The purpose of this study was to evaluate "in vivo'' safety of trypan blue (TB) in patients undergoing TB-assisted internal limiting membrane or epiretinal membrane peeling.Methods: Prospective study including 21 patients (21 eyes) with full-thickness macular hole and/or epiretinal membrane undergoing TB-assisted internal limiting membrane/epiretinal membrane peeling. Main outcome measures included distance visual acuity, near visual acuity, amplitude of P50 and N95 of the pattern electroretinogram, and fundus autofluorescence; these were assessed preoperatively, at 6 months (n = 21) and 12 months (n = 10) postoperatively.Results: There was a statistically significant improvement in distance visual acuity, near visual acuity, P50, and N95 amplitude at 6 months and 12 months postoperatively. The mean logarithm of the minimum angle of resolution distance visual acuity and near visual acuity improved from baseline by 0.31 (SD 0.37) and 0.17 (SD 0.31) at 6 months, respectively, and by 0.4 (SD 0.25) and 0.35 (SD 0.28) at 12 months, respectively. The mean P50 and N95 component amplitudes improved by 28% compared with baseline at 6 months (P50 0.4 [SD 0.8]; N95 0.53 [SD 1.07]) and by 63% at 12 months (P50 0.9 [0.85]; N95 1.04 [1.34]). Autofluorescence did not demonstrate damage to the retinal pigment epithelium attributable to TB.Conclusion: No deleterious effects of TB were observed in this study. RETINA 31:1122-1127, 2011

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KW - retinal function

KW - trypan blue

KW - internal limiting membrane

KW - macular hole surgery

KW - indocyanine green

KW - infracyanine green

KW - ISCEV standard

KW - electroretinography

KW - vitrectomy

KW - histology

KW - update

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DO - 10.1097/IAE.0b013e3182003adb

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VL - 31

SP - 1122

EP - 1127

JO - Retina

JF - Retina

SN - 0275-004X

IS - 6

ER -