Increased Bcl-2/p53 ratio in human osteoarthritic cartilage: a possible role in regulation of chondrocyte metabolism

F Iannone, Cosimo De Bari, C Scioscia, V Patella, G Lapadula

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Abstract

OBJECTIVE: To determine whether Bcl-2, p53, and Fas/CD95 help to control cartilage metabolism. METHODS: Six normal and 14 osteoarthritic (OA) cartilage samples were examined, and two zones from each sample showing the least (Min) and most (Max) anatomical damage were selected. Chondrocytes were isolated by sequential enzymatic digestion and freshly processed. Bcl-2, p53, and Fas/CD95 expression was evaluated by immunofluorescence and FACS analysis; the cell cycle was analysed using propidium iodide, and chondrocyte proliferation assessed by [(3)H]thymidine incorporation. RESULTS: Intracellular levels of Bcl-2 were significantly higher in Max (27.5%) than in Min (21%, p
Original languageEnglish
Pages (from-to)217-221
Number of pages5
JournalAnnals of the Rheumatic Diseases
Volume64
Issue number2
DOIs
Publication statusPublished - 1 Feb 2005

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Keywords

  • Adolescent
  • Adult
  • Aged
  • Antigens, CD95
  • Cartilage, Articular
  • Cell Cycle
  • Cell Division
  • Cells, Cultured
  • Chondrocytes
  • Humans
  • Middle Aged
  • Osteoarthritis, Knee
  • Proto-Oncogene Proteins c-bcl-2
  • Tumor Suppressor Protein p53

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