Increased Bcl-2/p53 ratio in human osteoarthritic cartilage: a possible role in regulation of chondrocyte metabolism

F Iannone, Cosimo De Bari, C Scioscia, V Patella, G Lapadula

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

OBJECTIVE: To determine whether Bcl-2, p53, and Fas/CD95 help to control cartilage metabolism. METHODS: Six normal and 14 osteoarthritic (OA) cartilage samples were examined, and two zones from each sample showing the least (Min) and most (Max) anatomical damage were selected. Chondrocytes were isolated by sequential enzymatic digestion and freshly processed. Bcl-2, p53, and Fas/CD95 expression was evaluated by immunofluorescence and FACS analysis; the cell cycle was analysed using propidium iodide, and chondrocyte proliferation assessed by [(3)H]thymidine incorporation. RESULTS: Intracellular levels of Bcl-2 were significantly higher in Max (27.5%) than in Min (21%, p
Original languageEnglish
Pages (from-to)217-221
Number of pages5
JournalAnnals of the Rheumatic Diseases
Volume64
Issue number2
DOIs
Publication statusPublished - 1 Feb 2005

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Cartilage
Chondrocytes
Metabolism
Propidium
Thymidine
Fluorescent Antibody Technique
Digestion
Cell Cycle
Cells

Keywords

  • Adolescent
  • Adult
  • Aged
  • Antigens, CD95
  • Cartilage, Articular
  • Cell Cycle
  • Cell Division
  • Cells, Cultured
  • Chondrocytes
  • Humans
  • Middle Aged
  • Osteoarthritis, Knee
  • Proto-Oncogene Proteins c-bcl-2
  • Tumor Suppressor Protein p53

Cite this

Increased Bcl-2/p53 ratio in human osteoarthritic cartilage : a possible role in regulation of chondrocyte metabolism. / Iannone, F; De Bari, Cosimo; Scioscia, C; Patella, V; Lapadula, G.

In: Annals of the Rheumatic Diseases, Vol. 64, No. 2, 01.02.2005, p. 217-221.

Research output: Contribution to journalArticle

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T2 - a possible role in regulation of chondrocyte metabolism

AU - Iannone, F

AU - De Bari, Cosimo

AU - Scioscia, C

AU - Patella, V

AU - Lapadula, G

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AB - OBJECTIVE: To determine whether Bcl-2, p53, and Fas/CD95 help to control cartilage metabolism. METHODS: Six normal and 14 osteoarthritic (OA) cartilage samples were examined, and two zones from each sample showing the least (Min) and most (Max) anatomical damage were selected. Chondrocytes were isolated by sequential enzymatic digestion and freshly processed. Bcl-2, p53, and Fas/CD95 expression was evaluated by immunofluorescence and FACS analysis; the cell cycle was analysed using propidium iodide, and chondrocyte proliferation assessed by [(3)H]thymidine incorporation. RESULTS: Intracellular levels of Bcl-2 were significantly higher in Max (27.5%) than in Min (21%, p

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KW - Aged

KW - Antigens, CD95

KW - Cartilage, Articular

KW - Cell Cycle

KW - Cell Division

KW - Cells, Cultured

KW - Chondrocytes

KW - Humans

KW - Middle Aged

KW - Osteoarthritis, Knee

KW - Proto-Oncogene Proteins c-bcl-2

KW - Tumor Suppressor Protein p53

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