Increased Bcl-2/p53 ratio in human osteoarthritic cartilage:  a possible role in regulation of chondrocyte metabolism

F Iannone; Cosimo De Bari; C Scioscia; V Patella; G Lapadula

doi:10.1136/ard.2004.022590

Increased Bcl-2/p53 ratio in human osteoarthritic cartilage: a possible role in regulation of chondrocyte metabolism

F Iannone, Cosimo De Bari, C Scioscia, V Patella, G Lapadula

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

OBJECTIVE: To determine whether Bcl-2, p53, and Fas/CD95 help to control cartilage metabolism. METHODS: Six normal and 14 osteoarthritic (OA) cartilage samples were examined, and two zones from each sample showing the least (Min) and most (Max) anatomical damage were selected. Chondrocytes were isolated by sequential enzymatic digestion and freshly processed. Bcl-2, p53, and Fas/CD95 expression was evaluated by immunofluorescence and FACS analysis; the cell cycle was analysed using propidium iodide, and chondrocyte proliferation assessed by [(3)H]thymidine incorporation. RESULTS: Intracellular levels of Bcl-2 were significantly higher in Max (27.5%) than in Min (21%, p

Original language	English
Pages (from-to)	217-221
Number of pages	5
Journal	Annals of the Rheumatic Diseases
Volume	64
Issue number	2
DOIs	https://doi.org/10.1136/ard.2004.022590
Publication status	Published - 1 Feb 2005

Keywords

Adolescent
Adult
Aged
Antigens, CD95
Cartilage, Articular
Cell Cycle
Cell Division
Cells, Cultured
Chondrocytes
Humans
Middle Aged
Osteoarthritis, Knee
Proto-Oncogene Proteins c-bcl-2
Tumor Suppressor Protein p53

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10.1136/ard.2004.022590

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title = "Increased Bcl-2/p53 ratio in human osteoarthritic cartilage: a possible role in regulation of chondrocyte metabolism",

abstract = "OBJECTIVE: To determine whether Bcl-2, p53, and Fas/CD95 help to control cartilage metabolism. METHODS: Six normal and 14 osteoarthritic (OA) cartilage samples were examined, and two zones from each sample showing the least (Min) and most (Max) anatomical damage were selected. Chondrocytes were isolated by sequential enzymatic digestion and freshly processed. Bcl-2, p53, and Fas/CD95 expression was evaluated by immunofluorescence and FACS analysis; the cell cycle was analysed using propidium iodide, and chondrocyte proliferation assessed by [(3)H]thymidine incorporation. RESULTS: Intracellular levels of Bcl-2 were significantly higher in Max (27.5%) than in Min (21%, p",

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author = "F Iannone and {De Bari}, Cosimo and C Scioscia and V Patella and G Lapadula",

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doi = "10.1136/ard.2004.022590",

language = "English",

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T1 - Increased Bcl-2/p53 ratio in human osteoarthritic cartilage

T2 - a possible role in regulation of chondrocyte metabolism

AU - Iannone, F

AU - De Bari, Cosimo

AU - Scioscia, C

AU - Patella, V

AU - Lapadula, G

PY - 2005/2/1

Y1 - 2005/2/1

N2 - OBJECTIVE: To determine whether Bcl-2, p53, and Fas/CD95 help to control cartilage metabolism. METHODS: Six normal and 14 osteoarthritic (OA) cartilage samples were examined, and two zones from each sample showing the least (Min) and most (Max) anatomical damage were selected. Chondrocytes were isolated by sequential enzymatic digestion and freshly processed. Bcl-2, p53, and Fas/CD95 expression was evaluated by immunofluorescence and FACS analysis; the cell cycle was analysed using propidium iodide, and chondrocyte proliferation assessed by [(3)H]thymidine incorporation. RESULTS: Intracellular levels of Bcl-2 were significantly higher in Max (27.5%) than in Min (21%, p

AB - OBJECTIVE: To determine whether Bcl-2, p53, and Fas/CD95 help to control cartilage metabolism. METHODS: Six normal and 14 osteoarthritic (OA) cartilage samples were examined, and two zones from each sample showing the least (Min) and most (Max) anatomical damage were selected. Chondrocytes were isolated by sequential enzymatic digestion and freshly processed. Bcl-2, p53, and Fas/CD95 expression was evaluated by immunofluorescence and FACS analysis; the cell cycle was analysed using propidium iodide, and chondrocyte proliferation assessed by [(3)H]thymidine incorporation. RESULTS: Intracellular levels of Bcl-2 were significantly higher in Max (27.5%) than in Min (21%, p

KW - Adolescent

KW - Adult

KW - Aged

KW - Antigens, CD95

KW - Cartilage, Articular

KW - Cell Cycle

KW - Cell Division

KW - Cells, Cultured

KW - Chondrocytes

KW - Humans

KW - Middle Aged

KW - Osteoarthritis, Knee

KW - Proto-Oncogene Proteins c-bcl-2

KW - Tumor Suppressor Protein p53

U2 - 10.1136/ard.2004.022590

DO - 10.1136/ard.2004.022590

M3 - Article

C2 - 15647429

SN - 0003-4967

VL - 64

SP - 217

EP - 221

JO - Annals of the Rheumatic Diseases

JF - Annals of the Rheumatic Diseases

IS - 2

ER -

Increased Bcl-2/p53 ratio in human osteoarthritic cartilage: a possible role in regulation of chondrocyte metabolism

Abstract

Keywords

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