Increased blood antioxidant systems of runners in response to training load

J D Robertson, R J Maughan, G G Duthie, P C Morrice

Research output: Contribution to journalArticle

206 Citations (Scopus)

Abstract

1. Blood antioxidants were measured in venous blood samples from 20 runners and six sedentary individuals. All subjects were male, between 20 and 40 years old, and in steady state with respect to body weight and physical activity patterns. Dietary analysis was undertaken using a 7-day weighed food intake. Correlations were sought between antioxidants in blood and (1) weekly training distance and (2) maximum oxygen uptake. In addition, 12 runners could be classified into two groups undertaking either low (range 16-43 km, n = 6) or high (80-147 km, n = 6) weekly training.

2. Body weight (range 55.3-90.0 kg) and percentage body fat (range 7-19%) both showed negative correlations with the weekly training distance (both P < 0.001). Energy intake and maximum oxygen uptake were both correlated with the weekly training distance (both P < 0.001).

3. Plasma creatine kinase activity, an indicator of muscle damage, was significantly correlated with the weekly training distance (P < 0.01), whereas the plasma concentration of thiobarbituric acid-reactive substances, an indicator of free-radical-mediated lipid peroxidation, decreased with increased maximum oxygen uptake (P < 0.01).

4. Erythrocyte alpha-tocopherol content was greater in the two running groups (P < 0.05) compared with the sedentary group, and lymphocyte ascorbic acid concentration was significantly elevated in the high-training group (P < 0.01) compared with the low-training group.

5. Erythrocyte activities of the antioxidant enzymes, glutathione peroxidase and catalase, were significantly and positively correlated with the weekly training distance (P < 0.01 and P < 0.05, respectively). Total erythrocyte glutathione content was higher in the two training groups, and was accounted for by an increase in reduced glutathione content.

6. These results indicate that there is an increase in blood antioxidant defence mechanisms associated with endurance training or the related life style, but despite this there is a degree of muscle damage in the training individuals.

Original languageEnglish
Pages (from-to)611-618
Number of pages8
JournalClinical Science
Volume80
Issue number6
DOIs
Publication statusPublished - Jun 1991

Keywords

  • antioxidants
  • exertion
  • vitamins
  • ascorbic-acid status
  • vitamin-E
  • lipid-peroxidation
  • skeletal-muscle
  • hydrogen-peroxide
  • glutathione-reductase
  • superoxide-dismutase
  • selenium deficiency
  • exercise
  • metabolism

Cite this

Increased blood antioxidant systems of runners in response to training load. / Robertson, J D; Maughan, R J; Duthie, G G; Morrice, P C.

In: Clinical Science, Vol. 80, No. 6, 06.1991, p. 611-618.

Research output: Contribution to journalArticle

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abstract = "1. Blood antioxidants were measured in venous blood samples from 20 runners and six sedentary individuals. All subjects were male, between 20 and 40 years old, and in steady state with respect to body weight and physical activity patterns. Dietary analysis was undertaken using a 7-day weighed food intake. Correlations were sought between antioxidants in blood and (1) weekly training distance and (2) maximum oxygen uptake. In addition, 12 runners could be classified into two groups undertaking either low (range 16-43 km, n = 6) or high (80-147 km, n = 6) weekly training.2. Body weight (range 55.3-90.0 kg) and percentage body fat (range 7-19{\%}) both showed negative correlations with the weekly training distance (both P < 0.001). Energy intake and maximum oxygen uptake were both correlated with the weekly training distance (both P < 0.001).3. Plasma creatine kinase activity, an indicator of muscle damage, was significantly correlated with the weekly training distance (P < 0.01), whereas the plasma concentration of thiobarbituric acid-reactive substances, an indicator of free-radical-mediated lipid peroxidation, decreased with increased maximum oxygen uptake (P < 0.01).4. Erythrocyte alpha-tocopherol content was greater in the two running groups (P < 0.05) compared with the sedentary group, and lymphocyte ascorbic acid concentration was significantly elevated in the high-training group (P < 0.01) compared with the low-training group.5. Erythrocyte activities of the antioxidant enzymes, glutathione peroxidase and catalase, were significantly and positively correlated with the weekly training distance (P < 0.01 and P < 0.05, respectively). Total erythrocyte glutathione content was higher in the two training groups, and was accounted for by an increase in reduced glutathione content.6. These results indicate that there is an increase in blood antioxidant defence mechanisms associated with endurance training or the related life style, but despite this there is a degree of muscle damage in the training individuals.",
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N2 - 1. Blood antioxidants were measured in venous blood samples from 20 runners and six sedentary individuals. All subjects were male, between 20 and 40 years old, and in steady state with respect to body weight and physical activity patterns. Dietary analysis was undertaken using a 7-day weighed food intake. Correlations were sought between antioxidants in blood and (1) weekly training distance and (2) maximum oxygen uptake. In addition, 12 runners could be classified into two groups undertaking either low (range 16-43 km, n = 6) or high (80-147 km, n = 6) weekly training.2. Body weight (range 55.3-90.0 kg) and percentage body fat (range 7-19%) both showed negative correlations with the weekly training distance (both P < 0.001). Energy intake and maximum oxygen uptake were both correlated with the weekly training distance (both P < 0.001).3. Plasma creatine kinase activity, an indicator of muscle damage, was significantly correlated with the weekly training distance (P < 0.01), whereas the plasma concentration of thiobarbituric acid-reactive substances, an indicator of free-radical-mediated lipid peroxidation, decreased with increased maximum oxygen uptake (P < 0.01).4. Erythrocyte alpha-tocopherol content was greater in the two running groups (P < 0.05) compared with the sedentary group, and lymphocyte ascorbic acid concentration was significantly elevated in the high-training group (P < 0.01) compared with the low-training group.5. Erythrocyte activities of the antioxidant enzymes, glutathione peroxidase and catalase, were significantly and positively correlated with the weekly training distance (P < 0.01 and P < 0.05, respectively). Total erythrocyte glutathione content was higher in the two training groups, and was accounted for by an increase in reduced glutathione content.6. These results indicate that there is an increase in blood antioxidant defence mechanisms associated with endurance training or the related life style, but despite this there is a degree of muscle damage in the training individuals.

AB - 1. Blood antioxidants were measured in venous blood samples from 20 runners and six sedentary individuals. All subjects were male, between 20 and 40 years old, and in steady state with respect to body weight and physical activity patterns. Dietary analysis was undertaken using a 7-day weighed food intake. Correlations were sought between antioxidants in blood and (1) weekly training distance and (2) maximum oxygen uptake. In addition, 12 runners could be classified into two groups undertaking either low (range 16-43 km, n = 6) or high (80-147 km, n = 6) weekly training.2. Body weight (range 55.3-90.0 kg) and percentage body fat (range 7-19%) both showed negative correlations with the weekly training distance (both P < 0.001). Energy intake and maximum oxygen uptake were both correlated with the weekly training distance (both P < 0.001).3. Plasma creatine kinase activity, an indicator of muscle damage, was significantly correlated with the weekly training distance (P < 0.01), whereas the plasma concentration of thiobarbituric acid-reactive substances, an indicator of free-radical-mediated lipid peroxidation, decreased with increased maximum oxygen uptake (P < 0.01).4. Erythrocyte alpha-tocopherol content was greater in the two running groups (P < 0.05) compared with the sedentary group, and lymphocyte ascorbic acid concentration was significantly elevated in the high-training group (P < 0.01) compared with the low-training group.5. Erythrocyte activities of the antioxidant enzymes, glutathione peroxidase and catalase, were significantly and positively correlated with the weekly training distance (P < 0.01 and P < 0.05, respectively). Total erythrocyte glutathione content was higher in the two training groups, and was accounted for by an increase in reduced glutathione content.6. These results indicate that there is an increase in blood antioxidant defence mechanisms associated with endurance training or the related life style, but despite this there is a degree of muscle damage in the training individuals.

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KW - hydrogen-peroxide

KW - glutathione-reductase

KW - superoxide-dismutase

KW - selenium deficiency

KW - exercise

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