Increased muscle proteolysis after local trauma mainly reflects macrophage-associated lysosomal proteolysis

M C Farges, Denis Pierre Balcerzak, B D Fisher, D Attaix, D Bechet, M Ferrara, V E Baracos

    Research output: Contribution to journalArticle

    52 Citations (Scopus)

    Abstract

    Rat gastrocnemius showed increased protein degradation (+75-115%) at 48 h after traumatic injury. Injured muscle showed increased cathepsin B activity (+327%) and mRNA encoding cathepsin B (+670%), cathepsin L (+298%), cathepsin H (+159%), and cathepsin C (+268%). In in situ hybridization, cathepsin B mRNA localized to the mononuclear cell infiltrate in injured muscle, and only background levels of hybridization were observed either over muscle cells in injured tissue or in uninjured muscle. Immunogold/electron microscopy showed specific staining for cathepsin B only in lysosome-like structures in cells of the mononuclear cell infiltrate in injured muscle. Muscle cells were uniformly negative in the immunocytochemistry. Matrix metalloproteinase-9 (granulocyte-macrophage gelatinase) mRNA and activity were not present in uninjured muscle but were expressed after trauma. There was no activation of the ATP-ubiquitin-proteasome-dependent proteolytic pathway in injured muscle, by contrast to diverse forms of muscle wasting where the activity of this system and the expression of genes encoding ubiquitin and proteasome elements rise. These results suggest that proteolytic systems of the muscle cells remain unstimulated after local injury and that lysosomal enzymes of the inflammatory infiltrated cells are likely to be the major participant in protein catabolism associated with local trauma.

    Original languageEnglish
    Number of pages10
    JournalAmerican Journal of Physiology: Endocrinology and Metabolism
    Volume282
    Issue number2
    Publication statusPublished - Feb 2002

    Keywords

    • injury
    • protein degradation
    • RAT SKELETAL-MUSCLE
    • CATHEPSIN-B
    • IMMUNOCYTOCHEMICAL LOCALIZATION
    • MATRIX METALLOPROTEINASES
    • MYOFIBRILLAR PROTEINS
    • METABOLIC RESPONSE
    • ENDOCRINE-CELLS
    • INHIBITORS
    • EXPRESSION
    • ACTIVATION

    Cite this

    Farges, M. C., Balcerzak, D. P., Fisher, B. D., Attaix, D., Bechet, D., Ferrara, M., & Baracos, V. E. (2002). Increased muscle proteolysis after local trauma mainly reflects macrophage-associated lysosomal proteolysis. American Journal of Physiology: Endocrinology and Metabolism, 282(2).

    Increased muscle proteolysis after local trauma mainly reflects macrophage-associated lysosomal proteolysis. / Farges, M C ; Balcerzak, Denis Pierre; Fisher, B D ; Attaix, D ; Bechet, D ; Ferrara, M ; Baracos, V E .

    In: American Journal of Physiology: Endocrinology and Metabolism, Vol. 282, No. 2, 02.2002.

    Research output: Contribution to journalArticle

    Farges, MC, Balcerzak, DP, Fisher, BD, Attaix, D, Bechet, D, Ferrara, M & Baracos, VE 2002, 'Increased muscle proteolysis after local trauma mainly reflects macrophage-associated lysosomal proteolysis', American Journal of Physiology: Endocrinology and Metabolism, vol. 282, no. 2.
    Farges, M C ; Balcerzak, Denis Pierre ; Fisher, B D ; Attaix, D ; Bechet, D ; Ferrara, M ; Baracos, V E . / Increased muscle proteolysis after local trauma mainly reflects macrophage-associated lysosomal proteolysis. In: American Journal of Physiology: Endocrinology and Metabolism. 2002 ; Vol. 282, No. 2.
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    AB - Rat gastrocnemius showed increased protein degradation (+75-115%) at 48 h after traumatic injury. Injured muscle showed increased cathepsin B activity (+327%) and mRNA encoding cathepsin B (+670%), cathepsin L (+298%), cathepsin H (+159%), and cathepsin C (+268%). In in situ hybridization, cathepsin B mRNA localized to the mononuclear cell infiltrate in injured muscle, and only background levels of hybridization were observed either over muscle cells in injured tissue or in uninjured muscle. Immunogold/electron microscopy showed specific staining for cathepsin B only in lysosome-like structures in cells of the mononuclear cell infiltrate in injured muscle. Muscle cells were uniformly negative in the immunocytochemistry. Matrix metalloproteinase-9 (granulocyte-macrophage gelatinase) mRNA and activity were not present in uninjured muscle but were expressed after trauma. There was no activation of the ATP-ubiquitin-proteasome-dependent proteolytic pathway in injured muscle, by contrast to diverse forms of muscle wasting where the activity of this system and the expression of genes encoding ubiquitin and proteasome elements rise. These results suggest that proteolytic systems of the muscle cells remain unstimulated after local injury and that lysosomal enzymes of the inflammatory infiltrated cells are likely to be the major participant in protein catabolism associated with local trauma.

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