Increased nuclear factor kappa B activation in critically ill patients who die

R L Paterson, H F Galley, J K Dhillon, N R Webster

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Objectives: To determine nuclear factor kappa B (NF kappa B) activation in mononuclear and neutrophils from critically ill patients and to compare NF kappa B activation with circulating concentrations of interleukin (IL)-6, IL-8, and soluble intercellular adhesion molecule (sICAM)-1.

Design: Observational study.

Setting: University Teaching Hospital, eight-bed intensive care unit in northeast Scotland.

Patients: Ten patients admitted to the intensive care unit who fulfilled the criteria for systemic inflammatory response syndrome were studied at 0, 24, 48, and 72 hrs. Six healthy volunteers were also studied.

Interventions: None.

Measurements and Main Results: NF kappa B activation was significantly higher in patients compared to healthy volunteers in both neutrophils (p =.001) and mononuclear leukocytes (p =.013). In the six patients who survived to 96 hrs, the level of NF kappa B activation in mononuclear cells remained constant (p =.9). However, in the four patients who died before 96 hrs, mononuclear cell NF kappa B activation increased markedly and was significantly higher before death than in those who survived to 96 hrs (p =.0105). NF kappa B activation in neutrophils similarly remained constant in patients who survived to 96 hrs (p =.4) but did not show the same increase before death. Circulating concentrations of IL-6, IL-8, and sICAM-1 were elevated but were unrelated to leukocyte NF kappa B activation.

Conclusions: We found NF kappa B activation in mononuclear and neutrophils in patients with systemic inflammatory response syndrome, which increased markedly before death in mononuclear leukocytes and was not related to plasma IL-6, IL-8, and sICAM-1 concentrations. These data support the need for further study of the role of NF kappa B activation in mortality from systemic inflammatory response syndrome and sepsis.

Original languageEnglish
Pages (from-to)1047-1051
Number of pages5
JournalCritical Care Medicine
Volume28
Publication statusPublished - 2000

Keywords

  • mononuclear leukocyte
  • neutrophil
  • systemic inflammatory response syndrome
  • interleukin-6
  • interleukin-8
  • soluble intercellular adhesion molecule-1
  • nuclear factor kappa B
  • INFLAMMATORY RESPONSE SYNDROME
  • SEPTIC SHOCK
  • TRANSCRIPTION FACTOR
  • CYTOKINE LEVELS
  • GENE
  • SEPSIS
  • INTERLEUKIN-6
  • EXPRESSION
  • MORTALITY
  • ENDOTOXIN

Cite this

Paterson, R. L., Galley, H. F., Dhillon, J. K., & Webster, N. R. (2000). Increased nuclear factor kappa B activation in critically ill patients who die. Critical Care Medicine, 28, 1047-1051.

Increased nuclear factor kappa B activation in critically ill patients who die. / Paterson, R L ; Galley, H F ; Dhillon, J K ; Webster, N R .

In: Critical Care Medicine, Vol. 28, 2000, p. 1047-1051.

Research output: Contribution to journalArticle

Paterson, RL, Galley, HF, Dhillon, JK & Webster, NR 2000, 'Increased nuclear factor kappa B activation in critically ill patients who die', Critical Care Medicine, vol. 28, pp. 1047-1051.
Paterson, R L ; Galley, H F ; Dhillon, J K ; Webster, N R . / Increased nuclear factor kappa B activation in critically ill patients who die. In: Critical Care Medicine. 2000 ; Vol. 28. pp. 1047-1051.
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TY - JOUR

T1 - Increased nuclear factor kappa B activation in critically ill patients who die

AU - Paterson, R L

AU - Galley, H F

AU - Dhillon, J K

AU - Webster, N R

PY - 2000

Y1 - 2000

N2 - Objectives: To determine nuclear factor kappa B (NF kappa B) activation in mononuclear and neutrophils from critically ill patients and to compare NF kappa B activation with circulating concentrations of interleukin (IL)-6, IL-8, and soluble intercellular adhesion molecule (sICAM)-1.Design: Observational study.Setting: University Teaching Hospital, eight-bed intensive care unit in northeast Scotland.Patients: Ten patients admitted to the intensive care unit who fulfilled the criteria for systemic inflammatory response syndrome were studied at 0, 24, 48, and 72 hrs. Six healthy volunteers were also studied.Interventions: None.Measurements and Main Results: NF kappa B activation was significantly higher in patients compared to healthy volunteers in both neutrophils (p =.001) and mononuclear leukocytes (p =.013). In the six patients who survived to 96 hrs, the level of NF kappa B activation in mononuclear cells remained constant (p =.9). However, in the four patients who died before 96 hrs, mononuclear cell NF kappa B activation increased markedly and was significantly higher before death than in those who survived to 96 hrs (p =.0105). NF kappa B activation in neutrophils similarly remained constant in patients who survived to 96 hrs (p =.4) but did not show the same increase before death. Circulating concentrations of IL-6, IL-8, and sICAM-1 were elevated but were unrelated to leukocyte NF kappa B activation.Conclusions: We found NF kappa B activation in mononuclear and neutrophils in patients with systemic inflammatory response syndrome, which increased markedly before death in mononuclear leukocytes and was not related to plasma IL-6, IL-8, and sICAM-1 concentrations. These data support the need for further study of the role of NF kappa B activation in mortality from systemic inflammatory response syndrome and sepsis.

AB - Objectives: To determine nuclear factor kappa B (NF kappa B) activation in mononuclear and neutrophils from critically ill patients and to compare NF kappa B activation with circulating concentrations of interleukin (IL)-6, IL-8, and soluble intercellular adhesion molecule (sICAM)-1.Design: Observational study.Setting: University Teaching Hospital, eight-bed intensive care unit in northeast Scotland.Patients: Ten patients admitted to the intensive care unit who fulfilled the criteria for systemic inflammatory response syndrome were studied at 0, 24, 48, and 72 hrs. Six healthy volunteers were also studied.Interventions: None.Measurements and Main Results: NF kappa B activation was significantly higher in patients compared to healthy volunteers in both neutrophils (p =.001) and mononuclear leukocytes (p =.013). In the six patients who survived to 96 hrs, the level of NF kappa B activation in mononuclear cells remained constant (p =.9). However, in the four patients who died before 96 hrs, mononuclear cell NF kappa B activation increased markedly and was significantly higher before death than in those who survived to 96 hrs (p =.0105). NF kappa B activation in neutrophils similarly remained constant in patients who survived to 96 hrs (p =.4) but did not show the same increase before death. Circulating concentrations of IL-6, IL-8, and sICAM-1 were elevated but were unrelated to leukocyte NF kappa B activation.Conclusions: We found NF kappa B activation in mononuclear and neutrophils in patients with systemic inflammatory response syndrome, which increased markedly before death in mononuclear leukocytes and was not related to plasma IL-6, IL-8, and sICAM-1 concentrations. These data support the need for further study of the role of NF kappa B activation in mortality from systemic inflammatory response syndrome and sepsis.

KW - mononuclear leukocyte

KW - neutrophil

KW - systemic inflammatory response syndrome

KW - interleukin-6

KW - interleukin-8

KW - soluble intercellular adhesion molecule-1

KW - nuclear factor kappa B

KW - INFLAMMATORY RESPONSE SYNDROME

KW - SEPTIC SHOCK

KW - TRANSCRIPTION FACTOR

KW - CYTOKINE LEVELS

KW - GENE

KW - SEPSIS

KW - INTERLEUKIN-6

KW - EXPRESSION

KW - MORTALITY

KW - ENDOTOXIN

M3 - Article

VL - 28

SP - 1047

EP - 1051

JO - Critical Care Medicine

JF - Critical Care Medicine

SN - 0090-3493

ER -