Indications of heightened constitutive or primed host response affecting the lignin pathway transcripts and phenolics in mature Norway spruce clones

C. G. Fossdal, N. Nagy, A. Hietela, H. Kvaalen, R. Slimestad, S. Woodward, H. Solheim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Two mature clones of Norway spruce (Picea abies (L.) Karst.) that have previously been shown to have differential degrees of resistance towards the necrotrophic pathogen Heterobasidion parviporum (Niemelä & Korhonen) were compared with respect to the primed defense expression of transcripts related to biosynthesis of lignin, stilbenes and other phenolic compounds from one year to the next. The host's response to physical wounding and pathogen inoculation was examined in the initial year, whereas indications of heightened basal defense level or primed response, and responses to re-wounding, were examined the following year. The responses of the two clones to wounding and pathogen inoculation, examined in the initial year, differed; the increases in lignin and phenolics were more distinct in response to the pathogen than to wounding alone. The more resistant clone 589 had higher initial lignin concentrations in the cell walls when compared with clone 409, and these remained higher in clone 589 over both years and increased after the treatments. Both clones responded at the transcriptional and chemical levels to wounding; changes were evident both in the initial wounds and when re-wounded the following year. There were distinct differences in the basal transcript levels of the lignin pathway-related genes, phenolics and total lignin levels in healthy tissue from the initial year to the following year indicative of a primed host response or at least altered constitutive level of defense expression.
Original languageEnglish
Pages (from-to)1137-1147
Number of pages11
JournalTree Physiology
Volume32
Issue number9
Early online date16 Aug 2012
DOIs
Publication statusPublished - Sep 2012

Fingerprint

Lignin
Norway
Picea abies
lignin
Clone Cells
clones
pathogens
Abies
Picea
Stilbenes
stilbenes
karsts
Cell Wall
phenolic compounds
cell walls
biosynthesis
Wounds and Injuries
Genes
genes

Keywords

  • Heterobasidion annosum
  • HPLC
  • lignin
  • phenolics
  • Picea abies
  • qRT-PCR
  • real-time RT-PCR
  • stilbenes
  • taxifolin

Cite this

Indications of heightened constitutive or primed host response affecting the lignin pathway transcripts and phenolics in mature Norway spruce clones. / Fossdal, C. G.; Nagy, N.; Hietela, A.; Kvaalen, H.; Slimestad, R.; Woodward, S.; Solheim, H.

In: Tree Physiology, Vol. 32, No. 9, 09.2012, p. 1137-1147.

Research output: Contribution to journalArticle

Fossdal, C. G. ; Nagy, N. ; Hietela, A. ; Kvaalen, H. ; Slimestad, R. ; Woodward, S. ; Solheim, H. / Indications of heightened constitutive or primed host response affecting the lignin pathway transcripts and phenolics in mature Norway spruce clones. In: Tree Physiology. 2012 ; Vol. 32, No. 9. pp. 1137-1147.
@article{a3c2cf0fe1ef4918822daadb4b4579cc,
title = "Indications of heightened constitutive or primed host response affecting the lignin pathway transcripts and phenolics in mature Norway spruce clones",
abstract = "Two mature clones of Norway spruce (Picea abies (L.) Karst.) that have previously been shown to have differential degrees of resistance towards the necrotrophic pathogen Heterobasidion parviporum (Niemel{\"a} & Korhonen) were compared with respect to the primed defense expression of transcripts related to biosynthesis of lignin, stilbenes and other phenolic compounds from one year to the next. The host's response to physical wounding and pathogen inoculation was examined in the initial year, whereas indications of heightened basal defense level or primed response, and responses to re-wounding, were examined the following year. The responses of the two clones to wounding and pathogen inoculation, examined in the initial year, differed; the increases in lignin and phenolics were more distinct in response to the pathogen than to wounding alone. The more resistant clone 589 had higher initial lignin concentrations in the cell walls when compared with clone 409, and these remained higher in clone 589 over both years and increased after the treatments. Both clones responded at the transcriptional and chemical levels to wounding; changes were evident both in the initial wounds and when re-wounded the following year. There were distinct differences in the basal transcript levels of the lignin pathway-related genes, phenolics and total lignin levels in healthy tissue from the initial year to the following year indicative of a primed host response or at least altered constitutive level of defense expression.",
keywords = "Heterobasidion annosum, HPLC, lignin, phenolics, Picea abies, qRT-PCR, real-time RT-PCR, stilbenes, taxifolin",
author = "Fossdal, {C. G.} and N. Nagy and A. Hietela and H. Kvaalen and R. Slimestad and S. Woodward and H. Solheim",
year = "2012",
month = "9",
doi = "10.1093/treephys/tps073",
language = "English",
volume = "32",
pages = "1137--1147",
journal = "Tree Physiology",
issn = "0829-318X",
publisher = "Oxford University Press",
number = "9",

}

TY - JOUR

T1 - Indications of heightened constitutive or primed host response affecting the lignin pathway transcripts and phenolics in mature Norway spruce clones

AU - Fossdal, C. G.

AU - Nagy, N.

AU - Hietela, A.

AU - Kvaalen, H.

AU - Slimestad, R.

AU - Woodward, S.

AU - Solheim, H.

PY - 2012/9

Y1 - 2012/9

N2 - Two mature clones of Norway spruce (Picea abies (L.) Karst.) that have previously been shown to have differential degrees of resistance towards the necrotrophic pathogen Heterobasidion parviporum (Niemelä & Korhonen) were compared with respect to the primed defense expression of transcripts related to biosynthesis of lignin, stilbenes and other phenolic compounds from one year to the next. The host's response to physical wounding and pathogen inoculation was examined in the initial year, whereas indications of heightened basal defense level or primed response, and responses to re-wounding, were examined the following year. The responses of the two clones to wounding and pathogen inoculation, examined in the initial year, differed; the increases in lignin and phenolics were more distinct in response to the pathogen than to wounding alone. The more resistant clone 589 had higher initial lignin concentrations in the cell walls when compared with clone 409, and these remained higher in clone 589 over both years and increased after the treatments. Both clones responded at the transcriptional and chemical levels to wounding; changes were evident both in the initial wounds and when re-wounded the following year. There were distinct differences in the basal transcript levels of the lignin pathway-related genes, phenolics and total lignin levels in healthy tissue from the initial year to the following year indicative of a primed host response or at least altered constitutive level of defense expression.

AB - Two mature clones of Norway spruce (Picea abies (L.) Karst.) that have previously been shown to have differential degrees of resistance towards the necrotrophic pathogen Heterobasidion parviporum (Niemelä & Korhonen) were compared with respect to the primed defense expression of transcripts related to biosynthesis of lignin, stilbenes and other phenolic compounds from one year to the next. The host's response to physical wounding and pathogen inoculation was examined in the initial year, whereas indications of heightened basal defense level or primed response, and responses to re-wounding, were examined the following year. The responses of the two clones to wounding and pathogen inoculation, examined in the initial year, differed; the increases in lignin and phenolics were more distinct in response to the pathogen than to wounding alone. The more resistant clone 589 had higher initial lignin concentrations in the cell walls when compared with clone 409, and these remained higher in clone 589 over both years and increased after the treatments. Both clones responded at the transcriptional and chemical levels to wounding; changes were evident both in the initial wounds and when re-wounded the following year. There were distinct differences in the basal transcript levels of the lignin pathway-related genes, phenolics and total lignin levels in healthy tissue from the initial year to the following year indicative of a primed host response or at least altered constitutive level of defense expression.

KW - Heterobasidion annosum

KW - HPLC

KW - lignin

KW - phenolics

KW - Picea abies

KW - qRT-PCR

KW - real-time RT-PCR

KW - stilbenes

KW - taxifolin

U2 - 10.1093/treephys/tps073

DO - 10.1093/treephys/tps073

M3 - Article

VL - 32

SP - 1137

EP - 1147

JO - Tree Physiology

JF - Tree Physiology

SN - 0829-318X

IS - 9

ER -