Induction of apoptosis and inhibition of NF-kappa B activation in human prostate cancer cells by the cis-9, trans-11 but not the trans-10, cis-12 isomer of conjugated linoleic acid

H J Song, Alan Arthur Sneddon, Steven Darryll Heys, K W J Wahle

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

BACKGROUND. Conjugated linoleic acids (CLAs) have anti-tumorigenic properties in animal models and anti-proliferative effects on cancer cells in vitro. Previous studies have shown that the NF-kappa B pathway is involved regulating anti-apoptotic gene expression. The present study investigated the effects of CLAs (cis-9, trans-11, and trans-10, cis-12 isomers and a 50:50 mixture) on apoptosis and NF-kappa B activation in LNCaP cells.
METHODS. Apoptosis was assessed by annexin V staining using flow cytometry. TNF-alpha-induced NF-kappa B activity was determined by gel shift and reporter gene assays in addition to monitoring I kappa B alpha phosphorylation.
RESULTS. Only the CLA cis-9, trans-11 isomer significantly increased TNF-alpha-induced apoptosis (by 59%), which correlated with a reduction in NF-kappa B transcriptional activity (by 35%, P < 0.05), NF-kappa B binding activity (by 15%, P < 0.05), and phosphorylation of I kappa B alpha (by 36%, P < 0.01).
CONCLUSIONS. Our results may offer a mechanistic explanation for the reported inhibition of prostate tumor growth by CLAs in animal models of disease.

Original languageEnglish
Pages (from-to)839-846
Number of pages8
JournalThe Prostate
Volume66
Issue number8
Early online date20 Feb 2006
DOIs
Publication statusPublished - 1 Jun 2006

Keywords

  • conjugated linoleic acids
  • apoptosis
  • NF-kappa B
  • I kappa B phosphorylation
  • prostate cancer cells
  • protein-kinase-C
  • mammary carcinogenesis
  • alpha
  • proliferation
  • isoforms
  • TNF
  • rat
  • involvement
  • survival
  • pathway

Cite this

Induction of apoptosis and inhibition of NF-kappa B activation in human prostate cancer cells by the cis-9, trans-11 but not the trans-10, cis-12 isomer of conjugated linoleic acid. / Song, H J ; Sneddon, Alan Arthur; Heys, Steven Darryll; Wahle, K W J .

In: The Prostate, Vol. 66, No. 8, 01.06.2006, p. 839-846.

Research output: Contribution to journalArticle

@article{45352251e85d4821b3e34304b55b91a7,
title = "Induction of apoptosis and inhibition of NF-kappa B activation in human prostate cancer cells by the cis-9, trans-11 but not the trans-10, cis-12 isomer of conjugated linoleic acid",
abstract = "BACKGROUND. Conjugated linoleic acids (CLAs) have anti-tumorigenic properties in animal models and anti-proliferative effects on cancer cells in vitro. Previous studies have shown that the NF-kappa B pathway is involved regulating anti-apoptotic gene expression. The present study investigated the effects of CLAs (cis-9, trans-11, and trans-10, cis-12 isomers and a 50:50 mixture) on apoptosis and NF-kappa B activation in LNCaP cells. METHODS. Apoptosis was assessed by annexin V staining using flow cytometry. TNF-alpha-induced NF-kappa B activity was determined by gel shift and reporter gene assays in addition to monitoring I kappa B alpha phosphorylation. RESULTS. Only the CLA cis-9, trans-11 isomer significantly increased TNF-alpha-induced apoptosis (by 59{\%}), which correlated with a reduction in NF-kappa B transcriptional activity (by 35{\%}, P < 0.05), NF-kappa B binding activity (by 15{\%}, P < 0.05), and phosphorylation of I kappa B alpha (by 36{\%}, P < 0.01). CONCLUSIONS. Our results may offer a mechanistic explanation for the reported inhibition of prostate tumor growth by CLAs in animal models of disease.",
keywords = "conjugated linoleic acids, apoptosis, NF-kappa B, I kappa B phosphorylation, prostate cancer cells, protein-kinase-C, mammary carcinogenesis, alpha, proliferation, isoforms, TNF, rat, involvement, survival, pathway",
author = "Song, {H J} and Sneddon, {Alan Arthur} and Heys, {Steven Darryll} and Wahle, {K W J}",
year = "2006",
month = "6",
day = "1",
doi = "10.1002/pros.20351",
language = "English",
volume = "66",
pages = "839--846",
journal = "The Prostate",
issn = "0270-4137",
publisher = "Wiley-Liss Inc.",
number = "8",

}

TY - JOUR

T1 - Induction of apoptosis and inhibition of NF-kappa B activation in human prostate cancer cells by the cis-9, trans-11 but not the trans-10, cis-12 isomer of conjugated linoleic acid

AU - Song, H J

AU - Sneddon, Alan Arthur

AU - Heys, Steven Darryll

AU - Wahle, K W J

PY - 2006/6/1

Y1 - 2006/6/1

N2 - BACKGROUND. Conjugated linoleic acids (CLAs) have anti-tumorigenic properties in animal models and anti-proliferative effects on cancer cells in vitro. Previous studies have shown that the NF-kappa B pathway is involved regulating anti-apoptotic gene expression. The present study investigated the effects of CLAs (cis-9, trans-11, and trans-10, cis-12 isomers and a 50:50 mixture) on apoptosis and NF-kappa B activation in LNCaP cells. METHODS. Apoptosis was assessed by annexin V staining using flow cytometry. TNF-alpha-induced NF-kappa B activity was determined by gel shift and reporter gene assays in addition to monitoring I kappa B alpha phosphorylation. RESULTS. Only the CLA cis-9, trans-11 isomer significantly increased TNF-alpha-induced apoptosis (by 59%), which correlated with a reduction in NF-kappa B transcriptional activity (by 35%, P < 0.05), NF-kappa B binding activity (by 15%, P < 0.05), and phosphorylation of I kappa B alpha (by 36%, P < 0.01). CONCLUSIONS. Our results may offer a mechanistic explanation for the reported inhibition of prostate tumor growth by CLAs in animal models of disease.

AB - BACKGROUND. Conjugated linoleic acids (CLAs) have anti-tumorigenic properties in animal models and anti-proliferative effects on cancer cells in vitro. Previous studies have shown that the NF-kappa B pathway is involved regulating anti-apoptotic gene expression. The present study investigated the effects of CLAs (cis-9, trans-11, and trans-10, cis-12 isomers and a 50:50 mixture) on apoptosis and NF-kappa B activation in LNCaP cells. METHODS. Apoptosis was assessed by annexin V staining using flow cytometry. TNF-alpha-induced NF-kappa B activity was determined by gel shift and reporter gene assays in addition to monitoring I kappa B alpha phosphorylation. RESULTS. Only the CLA cis-9, trans-11 isomer significantly increased TNF-alpha-induced apoptosis (by 59%), which correlated with a reduction in NF-kappa B transcriptional activity (by 35%, P < 0.05), NF-kappa B binding activity (by 15%, P < 0.05), and phosphorylation of I kappa B alpha (by 36%, P < 0.01). CONCLUSIONS. Our results may offer a mechanistic explanation for the reported inhibition of prostate tumor growth by CLAs in animal models of disease.

KW - conjugated linoleic acids

KW - apoptosis

KW - NF-kappa B

KW - I kappa B phosphorylation

KW - prostate cancer cells

KW - protein-kinase-C

KW - mammary carcinogenesis

KW - alpha

KW - proliferation

KW - isoforms

KW - TNF

KW - rat

KW - involvement

KW - survival

KW - pathway

U2 - 10.1002/pros.20351

DO - 10.1002/pros.20351

M3 - Article

VL - 66

SP - 839

EP - 846

JO - The Prostate

JF - The Prostate

SN - 0270-4137

IS - 8

ER -