Induction of hairy root cultures of Azadirachta indica A. Juss. and their production of azadirachtin and other important insect bioactive metabolites

Eunice Jessie Allan, J. P. Eeswara, A. P. Jarvis, Anne Jennifer Mordue, E. D. Morgan, Trevor Stuchbury

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Abstract

Hairy root cultures were established from stem and leaf explants of Azadirachta indica A. Juss (neem) following infection with Agrobacterium rhizogenes. Transformation was confirmed using polymerase chain reaction analysis. The cultures had a relatively fast growth rate (0.134 g dry weight day(-1)) and showed a 100-fold increase in biomass over a 4-week culture period. Both the roots and cultivation medium were antifeedant as determined by a no-choice bioassay using the desert locust, Schistocerca gregaria. Azadirachtin, nimbin, salannin, 3-acetyl-1-tigloylazadirachtinin and 3-tigloylazadirachtol were detected in the hairy roots by supercritical fluid chromatography. The cultivation medium contained 3-acetyl-1-tigloylazadirachtinin, 3-tigloylazadirachtol and desacetylsalannin.

Original languageEnglish
Pages (from-to)374-379
Number of pages5
JournalPlant Cell Reports
Volume21
DOIs
Publication statusPublished - 2002

Keywords

  • Azadirachta indica
  • neem
  • azadirachtin
  • hairy root cultures
  • NEEM
  • TRITERPENOIDS
  • CHROMATOGRAPHY
  • EXTRACTS
  • SEEDS
  • OIL

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