Induction of hairy root cultures of Azadirachta indica A. Juss. and their production of azadirachtin and other important insect bioactive metabolites

Eunice Jessie Allan; J. P. Eeswara; A. P. Jarvis; Anne Jennifer Mordue; E. D. Morgan; Trevor Stuchbury

doi:10.1007/s00299-002-0523-3

Induction of hairy root cultures of Azadirachta indica A. Juss. and their production of azadirachtin and other important insect bioactive metabolites

Eunice Jessie Allan, J. P. Eeswara, A. P. Jarvis, Anne Jennifer Mordue, E. D. Morgan, Trevor Stuchbury

Aberdeen Centre For Environmental Sustainability

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

Hairy root cultures were established from stem and leaf explants of Azadirachta indica A. Juss (neem) following infection with Agrobacterium rhizogenes. Transformation was confirmed using polymerase chain reaction analysis. The cultures had a relatively fast growth rate (0.134 g dry weight day(-1)) and showed a 100-fold increase in biomass over a 4-week culture period. Both the roots and cultivation medium were antifeedant as determined by a no-choice bioassay using the desert locust, Schistocerca gregaria. Azadirachtin, nimbin, salannin, 3-acetyl-1-tigloylazadirachtinin and 3-tigloylazadirachtol were detected in the hairy roots by supercritical fluid chromatography. The cultivation medium contained 3-acetyl-1-tigloylazadirachtinin, 3-tigloylazadirachtol and desacetylsalannin.

Original language	English
Pages (from-to)	374-379
Number of pages	5
Journal	Plant Cell Reports
Volume	21
DOIs	https://doi.org/10.1007/s00299-002-0523-3
Publication status	Published - 2002

Keywords

Azadirachta indica
neem
azadirachtin
hairy root cultures
NEEM
TRITERPENOIDS
CHROMATOGRAPHY
EXTRACTS
SEEDS
OIL

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@article{242338359be34bb78051895ec10a52a0,

title = "Induction of hairy root cultures of Azadirachta indica A. Juss. and their production of azadirachtin and other important insect bioactive metabolites",

abstract = "Hairy root cultures were established from stem and leaf explants of Azadirachta indica A. Juss (neem) following infection with Agrobacterium rhizogenes. Transformation was confirmed using polymerase chain reaction analysis. The cultures had a relatively fast growth rate (0.134 g dry weight day(-1)) and showed a 100-fold increase in biomass over a 4-week culture period. Both the roots and cultivation medium were antifeedant as determined by a no-choice bioassay using the desert locust, Schistocerca gregaria. Azadirachtin, nimbin, salannin, 3-acetyl-1-tigloylazadirachtinin and 3-tigloylazadirachtol were detected in the hairy roots by supercritical fluid chromatography. The cultivation medium contained 3-acetyl-1-tigloylazadirachtinin, 3-tigloylazadirachtol and desacetylsalannin.",

keywords = "Azadirachta indica, neem, azadirachtin, hairy root cultures, NEEM, TRITERPENOIDS, CHROMATOGRAPHY, EXTRACTS, SEEDS, OIL",

author = "Allan, {Eunice Jessie} and Eeswara, {J. P.} and Jarvis, {A. P.} and Mordue, {Anne Jennifer} and Morgan, {E. D.} and Trevor Stuchbury",

year = "2002",

doi = "10.1007/s00299-002-0523-3",

language = "English",

volume = "21",

pages = "374--379",

journal = "Plant Cell Reports",

issn = "0721-7714",

publisher = "Springer Verlag",

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TY - JOUR

T1 - Induction of hairy root cultures of Azadirachta indica A. Juss. and their production of azadirachtin and other important insect bioactive metabolites

AU - Allan, Eunice Jessie

AU - Eeswara, J. P.

AU - Jarvis, A. P.

AU - Mordue, Anne Jennifer

AU - Morgan, E. D.

AU - Stuchbury, Trevor

PY - 2002

Y1 - 2002

N2 - Hairy root cultures were established from stem and leaf explants of Azadirachta indica A. Juss (neem) following infection with Agrobacterium rhizogenes. Transformation was confirmed using polymerase chain reaction analysis. The cultures had a relatively fast growth rate (0.134 g dry weight day(-1)) and showed a 100-fold increase in biomass over a 4-week culture period. Both the roots and cultivation medium were antifeedant as determined by a no-choice bioassay using the desert locust, Schistocerca gregaria. Azadirachtin, nimbin, salannin, 3-acetyl-1-tigloylazadirachtinin and 3-tigloylazadirachtol were detected in the hairy roots by supercritical fluid chromatography. The cultivation medium contained 3-acetyl-1-tigloylazadirachtinin, 3-tigloylazadirachtol and desacetylsalannin.

AB - Hairy root cultures were established from stem and leaf explants of Azadirachta indica A. Juss (neem) following infection with Agrobacterium rhizogenes. Transformation was confirmed using polymerase chain reaction analysis. The cultures had a relatively fast growth rate (0.134 g dry weight day(-1)) and showed a 100-fold increase in biomass over a 4-week culture period. Both the roots and cultivation medium were antifeedant as determined by a no-choice bioassay using the desert locust, Schistocerca gregaria. Azadirachtin, nimbin, salannin, 3-acetyl-1-tigloylazadirachtinin and 3-tigloylazadirachtol were detected in the hairy roots by supercritical fluid chromatography. The cultivation medium contained 3-acetyl-1-tigloylazadirachtinin, 3-tigloylazadirachtol and desacetylsalannin.

KW - Azadirachta indica

KW - neem

KW - azadirachtin

KW - hairy root cultures

KW - NEEM

KW - TRITERPENOIDS

KW - CHROMATOGRAPHY

KW - EXTRACTS

KW - SEEDS

KW - OIL

U2 - 10.1007/s00299-002-0523-3

DO - 10.1007/s00299-002-0523-3

M3 - Article

SN - 0721-7714

VL - 21

SP - 374

EP - 379

JO - Plant Cell Reports

JF - Plant Cell Reports

ER -

Induction of hairy root cultures of Azadirachta indica A. Juss. and their production of azadirachtin and other important insect bioactive metabolites

Abstract

Keywords

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