Infection and transmission dynamics of rKSHV.219 in primary endothelial cells

Hannah C. Jeffery, Rachel L. Wheat, David J. Blackbourn, Gerard B. Nash, Lynn M. Butler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) is the aetiologic agent of Kaposi's sarcoma (KS), a tumour of endothelial cell origin. The study of KS development was aided by the generation of a recombinant GFP (latent)/RFP (lytic)-expressing KSHV (rKSHV.219) by Vieira and O'Hearn (2004). In this study the first data characterising primary endothelial cell infection and transmission with this virus is presented. Infection was predominantly latent and the percentage of GFP-positive cells increased over time. Neither horizontal transmission of infection, nor cellular proliferation, explained this increase. Analysis of latency-associated nuclear antigen (LANA-1) expression revealed that a threshold level of infection was required for GFP expression early post infection. At later time points GFP correlated more closely with LANA-1 expression, likely due to the accumulation of GFP over time. This study provides methodological guidance for the use of rKSHV.21. In addition, it highlights potential problems associated with the use of fluorescent proteins as markers of viral infection.

Original languageEnglish
Pages (from-to)251-259
Number of pages9
JournalJournal of Virological Methods
Volume193
Issue number1
DOIs
Publication statusPublished - Oct 2013

Keywords

  • Endothelial cell
  • Kaposi's sarcoma-associated herpes virus
  • Rkshv.219

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