The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1- enyl]pyrrolidine-2-carboxylic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high-activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.
|Number of pages||3|
|Journal||Applied and Environmental Microbiology|
|Publication status||Published - Jul 1999|
Floret, F., McKain, N., Newbold, C. J., Wallace, R. J., Chaudhary, L. C., Ellis, W. C., & El Hassan, S. (1999). Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms. Applied and Environmental Microbiology, 65(7), 3258-3260. https://doi.org/http://aem.asm.org/content/65/7/3258.long