Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

F. Floret, N. McKain, C. J. Newbold, R. J. Wallace, L. C. Chaudhary, W. C. Ellis, S. El Hassan

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The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1- enyl]pyrrolidine-2-carboxylic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high-activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.
Original languageEnglish
Pages (from-to)3258-3260
Number of pages3
JournalApplied and Environmental Microbiology
Issue number7
Publication statusPublished - Jul 1999


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