Influence of bone affinity on the skeletal distribution of fluorescently labeled bisphosphonates in vivo

Anke J Roelofs, Charlotte A Stewart, Shuting Sun, Katarzyna M Blazewska, Boris A Kashemirov, Charles E McKenna, R Graham G Russell, Michael J Rogers, Mark W Lundy, Frank H Ebetino, Fraser Coxon

Research output: Contribution to journalArticle

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Abstract

Bisphosphonates are widely used anti-resorptive drugs that bind to calcium. It has become evident that these drugs have differing affinities for bone mineral, however it is unclear whether such differences affect their distribution on mineral surfaces. In this study, fluorescent conjugates of risedronate, and its lower affinity analogues deoxy-risedronate and 3-PEHPC, were used to compare the localization of compounds with differing mineral affinities in vivo. Binding to dentine in vitro confirmed differences in mineral binding between compounds, which was influenced predominantly by the characteristics of the parent compound, but also by the choice of fluorescent tag. In growing rats, all compounds preferentially bound to forming endocortical as opposed to resorbing periosteal surfaces in cortical bone. At resorbing surfaces, lower affinity compounds showed preferential binding to resorption lacunae, while the highest affinity compound showed more uniform labeling. At forming surfaces, penetration into the mineralizing osteoid was found to inversely correlate with mineral affinity. These differences in distribution at resorbing and forming surfaces were not observed at quiescent surfaces. Lower affinity compounds also showed a relatively higher degree of labeling of osteocyte lacunar walls, and labeled lacunae deeper within cortical bone, indicating increased penetration of the osteocyte canalicular network. Similar differences in mineralizing surface and osteocyte network penetration between high and low affinity compounds were evident 7 days after administration, with fluorescent conjugates at forming surfaces buried under a new layer of bone. Fluorescent compounds were incorporated into these areas of newly-formed bone, indicating that 'recycling' had occurred, albeit at very low levels. Taken together, these findings indicate that the bone mineral affinity of bisphosphonates is likely to influence their distribution within the skeleton.
Original languageEnglish
Pages (from-to)835-847
Number of pages13
JournalJournal of Bone and Mineral Research
Volume27
Issue number4
Early online date20 Mar 2012
DOIs
Publication statusPublished - Apr 2012

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Diphosphonates
Minerals
Bone and Bones
Osteocytes
Recycling
Dentin
Skeleton
Pharmaceutical Preparations
Calcium

Keywords

  • bisphosphonates
  • bone affinity
  • distribution
  • osteocytes
  • bone resorption

Cite this

Influence of bone affinity on the skeletal distribution of fluorescently labeled bisphosphonates in vivo. / Roelofs, Anke J; Stewart, Charlotte A; Sun, Shuting; Blazewska, Katarzyna M; Kashemirov, Boris A; McKenna, Charles E; Russell, R Graham G; Rogers, Michael J; Lundy, Mark W; Ebetino, Frank H; Coxon, Fraser.

In: Journal of Bone and Mineral Research, Vol. 27, No. 4, 04.2012, p. 835-847.

Research output: Contribution to journalArticle

Roelofs, AJ, Stewart, CA, Sun, S, Blazewska, KM, Kashemirov, BA, McKenna, CE, Russell, RGG, Rogers, MJ, Lundy, MW, Ebetino, FH & Coxon, F 2012, 'Influence of bone affinity on the skeletal distribution of fluorescently labeled bisphosphonates in vivo', Journal of Bone and Mineral Research, vol. 27, no. 4, pp. 835-847. https://doi.org/10.1002/jbmr.1543
Roelofs, Anke J ; Stewart, Charlotte A ; Sun, Shuting ; Blazewska, Katarzyna M ; Kashemirov, Boris A ; McKenna, Charles E ; Russell, R Graham G ; Rogers, Michael J ; Lundy, Mark W ; Ebetino, Frank H ; Coxon, Fraser. / Influence of bone affinity on the skeletal distribution of fluorescently labeled bisphosphonates in vivo. In: Journal of Bone and Mineral Research. 2012 ; Vol. 27, No. 4. pp. 835-847.
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AU - Sun, Shuting

AU - Blazewska, Katarzyna M

AU - Kashemirov, Boris A

AU - McKenna, Charles E

AU - Russell, R Graham G

AU - Rogers, Michael J

AU - Lundy, Mark W

AU - Ebetino, Frank H

AU - Coxon, Fraser

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N2 - Bisphosphonates are widely used anti-resorptive drugs that bind to calcium. It has become evident that these drugs have differing affinities for bone mineral, however it is unclear whether such differences affect their distribution on mineral surfaces. In this study, fluorescent conjugates of risedronate, and its lower affinity analogues deoxy-risedronate and 3-PEHPC, were used to compare the localization of compounds with differing mineral affinities in vivo. Binding to dentine in vitro confirmed differences in mineral binding between compounds, which was influenced predominantly by the characteristics of the parent compound, but also by the choice of fluorescent tag. In growing rats, all compounds preferentially bound to forming endocortical as opposed to resorbing periosteal surfaces in cortical bone. At resorbing surfaces, lower affinity compounds showed preferential binding to resorption lacunae, while the highest affinity compound showed more uniform labeling. At forming surfaces, penetration into the mineralizing osteoid was found to inversely correlate with mineral affinity. These differences in distribution at resorbing and forming surfaces were not observed at quiescent surfaces. Lower affinity compounds also showed a relatively higher degree of labeling of osteocyte lacunar walls, and labeled lacunae deeper within cortical bone, indicating increased penetration of the osteocyte canalicular network. Similar differences in mineralizing surface and osteocyte network penetration between high and low affinity compounds were evident 7 days after administration, with fluorescent conjugates at forming surfaces buried under a new layer of bone. Fluorescent compounds were incorporated into these areas of newly-formed bone, indicating that 'recycling' had occurred, albeit at very low levels. Taken together, these findings indicate that the bone mineral affinity of bisphosphonates is likely to influence their distribution within the skeleton.

AB - Bisphosphonates are widely used anti-resorptive drugs that bind to calcium. It has become evident that these drugs have differing affinities for bone mineral, however it is unclear whether such differences affect their distribution on mineral surfaces. In this study, fluorescent conjugates of risedronate, and its lower affinity analogues deoxy-risedronate and 3-PEHPC, were used to compare the localization of compounds with differing mineral affinities in vivo. Binding to dentine in vitro confirmed differences in mineral binding between compounds, which was influenced predominantly by the characteristics of the parent compound, but also by the choice of fluorescent tag. In growing rats, all compounds preferentially bound to forming endocortical as opposed to resorbing periosteal surfaces in cortical bone. At resorbing surfaces, lower affinity compounds showed preferential binding to resorption lacunae, while the highest affinity compound showed more uniform labeling. At forming surfaces, penetration into the mineralizing osteoid was found to inversely correlate with mineral affinity. These differences in distribution at resorbing and forming surfaces were not observed at quiescent surfaces. Lower affinity compounds also showed a relatively higher degree of labeling of osteocyte lacunar walls, and labeled lacunae deeper within cortical bone, indicating increased penetration of the osteocyte canalicular network. Similar differences in mineralizing surface and osteocyte network penetration between high and low affinity compounds were evident 7 days after administration, with fluorescent conjugates at forming surfaces buried under a new layer of bone. Fluorescent compounds were incorporated into these areas of newly-formed bone, indicating that 'recycling' had occurred, albeit at very low levels. Taken together, these findings indicate that the bone mineral affinity of bisphosphonates is likely to influence their distribution within the skeleton.

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KW - bone affinity

KW - distribution

KW - osteocytes

KW - bone resorption

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JO - Journal of Bone and Mineral Research

JF - Journal of Bone and Mineral Research

SN - 0884-0431

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ER -