Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids

I Wasowska, M R G Maia, K M Niedzwiedzka, M Czauderna, J M C R Ribeiro, E Devillard, K J Shingfield, R J Wallace

Research output: Contribution to journalArticle

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Abstract

Dietary cis-9, trans-11-conjugated linoleic acid (CLA) is generally thought to be beneficial for human health. Fish oil added to ruminant diets increases the CLA concentration of milk and meat, an increase thought to arise from alterations in ruminal biohydrogenation of unsaturated fatty acids. To investigate the mechanism for this effect, in vitro incubations were carried out with ruminal digesta and the main biohydrogenating ruminal bacterium, Butyrivibrio fibrisolvens. Linoleic acid (LA) or alpha-linolenic acid (LNA) was incubated (1.67 g/l) with strained ruminal digesta from sheep receiving a 50:50 grass hay-concentrate ration. Adding fish oil (up to 4.17 g/l) tended to decrease the initial rate of LA (P=0.025) and LNA (P=0.137) disappearance, decreased (P < 0.05) the transient accumulation of conjugated isomers of both fatty acids, and increased (P < 0.05) the accumulation of trans-11-18:1. Concentrations of EPA (20:5n-3) or DHA (22:6n-3), the major fatty acids in fish oil, were low (100 mg/l or less) after incubation of fish oil with ruminal digesta. Addition of EPA or DHA (50 mg/l) to pure cultures inhibited the growth and isomerase activity of B. fibrisolvens, while fish oil had no effect. In contrast, similar concentrations of EPA and DHA had no effect on biohydrogenation of LA by mixed digesta, while the addition of LA prevented metabolism of EPA and DHA. Neither EPA nor DHA was metabolised by B. fibrisolvens in pure culture. Thus, fish oil inhibits ruminal biohydrogenation by a mechanism which can be interpreted partly, but not entirely, in terms of its effects on B. fibrisolvens.

Original languageEnglish
Pages (from-to)1199-1211
Number of pages13
JournalBritish Journal of Nutrition
Volume95
Issue number6
DOIs
Publication statusPublished - Jun 2006

Keywords

  • biohydrogenation
  • conjugated linoleic acid
  • fish oil
  • trans fatty acids
  • chromatography mass-spectrometry
  • lactating dairy cows
  • bovine milk
  • in vitro
  • butyrivibrio-fibrisolvens
  • rumen bacteria
  • adipose tissue
  • sunflower oil
  • dietary

Cite this

Wasowska, I., Maia, M. R. G., Niedzwiedzka, K. M., Czauderna, M., Ribeiro, J. M. C. R., Devillard, E., ... Wallace, R. J. (2006). Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids. British Journal of Nutrition, 95(6), 1199-1211. https://doi.org/10.1079/BJN20061783

Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids. / Wasowska, I; Maia, M R G; Niedzwiedzka, K M ; Czauderna, M; Ribeiro, J M C R ; Devillard, E; Shingfield, K J; Wallace, R J.

In: British Journal of Nutrition, Vol. 95, No. 6, 06.2006, p. 1199-1211.

Research output: Contribution to journalArticle

Wasowska, I, Maia, MRG, Niedzwiedzka, KM, Czauderna, M, Ribeiro, JMCR, Devillard, E, Shingfield, KJ & Wallace, RJ 2006, 'Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids', British Journal of Nutrition, vol. 95, no. 6, pp. 1199-1211. https://doi.org/10.1079/BJN20061783
Wasowska I, Maia MRG, Niedzwiedzka KM, Czauderna M, Ribeiro JMCR, Devillard E et al. Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids. British Journal of Nutrition. 2006 Jun;95(6):1199-1211. https://doi.org/10.1079/BJN20061783
Wasowska, I ; Maia, M R G ; Niedzwiedzka, K M ; Czauderna, M ; Ribeiro, J M C R ; Devillard, E ; Shingfield, K J ; Wallace, R J. / Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids. In: British Journal of Nutrition. 2006 ; Vol. 95, No. 6. pp. 1199-1211.
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abstract = "Dietary cis-9, trans-11-conjugated linoleic acid (CLA) is generally thought to be beneficial for human health. Fish oil added to ruminant diets increases the CLA concentration of milk and meat, an increase thought to arise from alterations in ruminal biohydrogenation of unsaturated fatty acids. To investigate the mechanism for this effect, in vitro incubations were carried out with ruminal digesta and the main biohydrogenating ruminal bacterium, Butyrivibrio fibrisolvens. Linoleic acid (LA) or alpha-linolenic acid (LNA) was incubated (1.67 g/l) with strained ruminal digesta from sheep receiving a 50:50 grass hay-concentrate ration. Adding fish oil (up to 4.17 g/l) tended to decrease the initial rate of LA (P=0.025) and LNA (P=0.137) disappearance, decreased (P < 0.05) the transient accumulation of conjugated isomers of both fatty acids, and increased (P < 0.05) the accumulation of trans-11-18:1. Concentrations of EPA (20:5n-3) or DHA (22:6n-3), the major fatty acids in fish oil, were low (100 mg/l or less) after incubation of fish oil with ruminal digesta. Addition of EPA or DHA (50 mg/l) to pure cultures inhibited the growth and isomerase activity of B. fibrisolvens, while fish oil had no effect. In contrast, similar concentrations of EPA and DHA had no effect on biohydrogenation of LA by mixed digesta, while the addition of LA prevented metabolism of EPA and DHA. Neither EPA nor DHA was metabolised by B. fibrisolvens in pure culture. Thus, fish oil inhibits ruminal biohydrogenation by a mechanism which can be interpreted partly, but not entirely, in terms of its effects on B. fibrisolvens.",
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AU - Maia, M R G

AU - Niedzwiedzka, K M

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AU - Ribeiro, J M C R

AU - Devillard, E

AU - Shingfield, K J

AU - Wallace, R J

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N2 - Dietary cis-9, trans-11-conjugated linoleic acid (CLA) is generally thought to be beneficial for human health. Fish oil added to ruminant diets increases the CLA concentration of milk and meat, an increase thought to arise from alterations in ruminal biohydrogenation of unsaturated fatty acids. To investigate the mechanism for this effect, in vitro incubations were carried out with ruminal digesta and the main biohydrogenating ruminal bacterium, Butyrivibrio fibrisolvens. Linoleic acid (LA) or alpha-linolenic acid (LNA) was incubated (1.67 g/l) with strained ruminal digesta from sheep receiving a 50:50 grass hay-concentrate ration. Adding fish oil (up to 4.17 g/l) tended to decrease the initial rate of LA (P=0.025) and LNA (P=0.137) disappearance, decreased (P < 0.05) the transient accumulation of conjugated isomers of both fatty acids, and increased (P < 0.05) the accumulation of trans-11-18:1. Concentrations of EPA (20:5n-3) or DHA (22:6n-3), the major fatty acids in fish oil, were low (100 mg/l or less) after incubation of fish oil with ruminal digesta. Addition of EPA or DHA (50 mg/l) to pure cultures inhibited the growth and isomerase activity of B. fibrisolvens, while fish oil had no effect. In contrast, similar concentrations of EPA and DHA had no effect on biohydrogenation of LA by mixed digesta, while the addition of LA prevented metabolism of EPA and DHA. Neither EPA nor DHA was metabolised by B. fibrisolvens in pure culture. Thus, fish oil inhibits ruminal biohydrogenation by a mechanism which can be interpreted partly, but not entirely, in terms of its effects on B. fibrisolvens.

AB - Dietary cis-9, trans-11-conjugated linoleic acid (CLA) is generally thought to be beneficial for human health. Fish oil added to ruminant diets increases the CLA concentration of milk and meat, an increase thought to arise from alterations in ruminal biohydrogenation of unsaturated fatty acids. To investigate the mechanism for this effect, in vitro incubations were carried out with ruminal digesta and the main biohydrogenating ruminal bacterium, Butyrivibrio fibrisolvens. Linoleic acid (LA) or alpha-linolenic acid (LNA) was incubated (1.67 g/l) with strained ruminal digesta from sheep receiving a 50:50 grass hay-concentrate ration. Adding fish oil (up to 4.17 g/l) tended to decrease the initial rate of LA (P=0.025) and LNA (P=0.137) disappearance, decreased (P < 0.05) the transient accumulation of conjugated isomers of both fatty acids, and increased (P < 0.05) the accumulation of trans-11-18:1. Concentrations of EPA (20:5n-3) or DHA (22:6n-3), the major fatty acids in fish oil, were low (100 mg/l or less) after incubation of fish oil with ruminal digesta. Addition of EPA or DHA (50 mg/l) to pure cultures inhibited the growth and isomerase activity of B. fibrisolvens, while fish oil had no effect. In contrast, similar concentrations of EPA and DHA had no effect on biohydrogenation of LA by mixed digesta, while the addition of LA prevented metabolism of EPA and DHA. Neither EPA nor DHA was metabolised by B. fibrisolvens in pure culture. Thus, fish oil inhibits ruminal biohydrogenation by a mechanism which can be interpreted partly, but not entirely, in terms of its effects on B. fibrisolvens.

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KW - conjugated linoleic acid

KW - fish oil

KW - trans fatty acids

KW - chromatography mass-spectrometry

KW - lactating dairy cows

KW - bovine milk

KW - in vitro

KW - butyrivibrio-fibrisolvens

KW - rumen bacteria

KW - adipose tissue

KW - sunflower oil

KW - dietary

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DO - 10.1079/BJN20061783

M3 - Article

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JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

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